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  • Article title
  • Abstract
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  • Introduction
  • Materials and methods
    • Chemical and cell cultures
    • Production of REO/NE using high-pressure homogenization technique
    • Preparation of REO/NE+MC
    • Determination of particle size and charge
    • Determination of cell growth inhibition with MTT assay
    • Determination of synergy
    • Assessment of nuclear morphology of apoptotic cells with DAPI staining
    • Statistical analysis
  • Results
    • Formulation of rosemary oil in water nano-emulsion (REO/NE)
    • Physical characteristics of REO/NE and REO/NE+MC using Zetasizer
    • Effects of MC, REO/NE and their combination on the viability of MCF-7 cells and HeLa cells
    • Effect of combination of REO/NE and MC on MCF-7 and HeLa cells
    • Treatment with REO/NE + MC induced nuclear morphology change and apoptosis
  • Discussion
  • Conclusion
  • References
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