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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Materials and methods
    • Apparatus
    • Chemical reagents
    • Standard ESM solution (100 μg/mL)
      • Hydrochloric acid solution (approximately 1 M)
    • ESM injection solution (100 μg/mL)
    • The general principle of the procedure
    • The wavelength for ESM in the UV spectrum
  • Results and discussion
    • Optimum conditions for the reaction
    • Effect of acid solution
    • The effect of volume hydrochloric acid
    • The duration of development and stability
    • Absorption spectra
    • Recommended procedure and calibration curve
    • Accuracy and precision
    • Application of the method
    • Quantification
    • Evaluation of the suggested procedure
    • Green analytical chemistry
    • Compare the proposed method with other UV methods
  • Conclusion
  • Acknowledgments
  • Additional information
  • References
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