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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Materials and methods
    • Chemicals and instruments
    • Plant material
    • Preliminary extraction experiments
    • Optimization of the extraction procedure
    • Analysis of samples through UFLC-DAD
    • Standard solutions
    • Validation quantitative method
    • Applications
    • Statistical analyses
  • Results and disscussion
    • Preliminary extraction experiments
    • Optimization of the extraction procedure by RSM
      • Model fitting
      • Effect of the extraction variables on TPC
      • Effect of the extraction variables on TFC
    • Optimization of the extraction conditions
    • Quantification method and validation
      • Selectivity
      • Linearity and limits of detection and quantification
      • Precision
      • Accuracy
    • Application to samples
  • Conclusions
  • Acknowledgements
  • References
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