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  • Article title
  • Abstract
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  • Introduction
  • Materials and methods
    • Material
    • Method
      • Factorial design to optimise the formulation
    • Data analysis and desirability function
    • Preparation of Candesartan cilexe­til-loaded nanosuspension
    • Determination of melting point
    • Determination of the maximum λmax of candesartan cilexetil
    • Construction calibration curves of candesartan cilexetil
    • Saturated solubility of candesartan cilexetil
    • Particle size and polydispersity index analysis
    • Determination of entrapment efficiency (EE%) of candesartan cilexetil
    • Development and evaluation of the optimised formulation
    • Freeze drying of nanosuspension
    • Morphological studies using AFM analy­sis
    • Compatibility analysis using DSC analysis
    • Drug-excipient compatibility studies using FTIR
    • In vitro release study
    • Stability study
    • Statistical analysis
  • Results and discussion
    • Melting point of candesartan cilexetil
    • Maximum λmax of drug candesartan cilexetil
    • Calibration curves of candesartan cilexe­til in (pH 1.2) and (pH 6.8)
    • Saturated solubility of candesartan cilexetil
    • Particle size, PDI, EE% and DL% analysis
    • Experimental design and analysis
    • Optimisation and evaluation of the prepared formulations
    • Morphological studies using AFM analysis
    • Drug-excipient compatibility studies using FTIR
    • Compatibility analysis using DSC analysis
    • In vitro release study
    • Stability study
  • Conclusions
  • References
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