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Article title
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Abstract
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Keywords
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Introduction
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Materials and methods
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Chemicals, cell lines and culture conditions
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Commercial kits
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Preparation of epigallocatechin Gallate, baicalin, and vincristine working solutions
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Antiproliferative assay
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Calculation of inhibitory concentration (IC₅₀)
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Calculation of resistance fold
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Colorimetric assay of caspase-3 activity in EMT-6/V cells
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Mice
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Establishing regimens for the in vivo experiment
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Tumor inoculation and antitumor activity in vivo
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Evaluation of liver function in treated mice
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Evaluation of kidney function in treated mice
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Data analysis
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Results
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In vitro results
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Anti-proliferative effect of vincristine on EMT-6/P and EMT-6/V cell line
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Anti-proliferative effect of Epigallocatechin Gallate and Baicalin combination treatment on EMT-6/P, EMT-6/V cell lines
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Combination index (CI) calculations
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Resistance fold
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In vivo results
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Antitumor effects of Baicalin, Epigallocatechin Gallate, their combination, vincristine, and vincristine together with the combination on tumor size implanted in mice on EMT-6/P and EMT-6/V
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Liver toxicity evaluation in mice
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Kidney toxicity evaluation in mice
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Discussion
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Conclusion
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Recommendations
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Funding sources
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Author contributions
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Ethical approval
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Acknowledgments
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References
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