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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Materials and methods
    • Materials
    • Development of the HPLC method
    • Validation of the HPLC method
    • System suitability
    • Linearity
    • Accuracy
    • Precision
    • Specificity
    • LOD and LOQ
    • Robustness
    • Stressed degradation
    • Nanoparticles preparation
    • Characterisation of loaded nanoparticles
      • Zeta potential and particle size investigation
      • Estimation of drug content and entrapment efficiency
      • Fourier transform infrared spectroscopy (FTIR)
      • In vitro release
      • Morphology investigation
      • Statistical analysis
  • Results and discussion
    • HPLC method and validation
      • Specificity
    • Characterization of loaded nanoparticles
      • Zeta potential and particle size investigation
      • Content and entrapment efficiency
      • Fourier transform infrared spectroscopy (FTIR)
      • In vitro release
      • Morphology investigation
  • Conclusion
  • Acknowledgement
  • References
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