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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Materials and methods
    • Plant material
    • Chemicals and reagents
    • Equipment and instruments
    • Preparation of plant extracts
    • Extraction methods
    • Optimization of the extraction methods
    • Determination of total phenolic content
    • Determination of total flavonoid content
    • HPLC analysis
      • HPLC chromatographic system and conditions
      • Standard solution preparation
      • Sample solution preparation
    • Cytotoxicity assay
      • Cell culture
      • MTT cell proliferation assay antiproliferative
  • Results and discussion
    • Optimization of the extraction
    • Determination of total phenolic content (TPC)
    • Determination of total flavonoid content (TFC)
    • HPLC analysis
      • Calibration curves
      • HPLC analysis of selected flavonoids and phenolic compounds
    • MTT cell proliferation assay antiproliferative
  • Conclusions and future recommendations
  • References
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