2024-03-29T12:03:59Z
https://pharmacia.pensoft.net/oai.php
10.3897/pharmacia.66.e35643
2019-05-14
pharmacia
Medical University, Sofia, Bulgaria
author
Peikova, Lily
https://orcid.org/0000-0002-8030-3479
Medical University, Sofia, Bulgaria
author
Tzankova, Diava
Medical University, Sofia, Bulgaria
author
Mateeva, Alexandrina
https://orcid.org/0000-0003-1905-7130
Medical University, Sofia, Bulgaria
author
Georgieva, Maya
Medical University, Sofia, Bulgaria
author
Zlatkov, Alexander
https://orcid.org/0000-0002-1695-8454
2019-05-14
2019-05-14
2019
Pharmacia
2603-557X
0428-0296
66
1
1-6
2019
10.3897/pharmacia.66.e35643
https://pharmacia.pensoft.net/article/35643/
https://pharmacia.pensoft.net/article/35643/download/pdf/
https://pharmacia.pensoft.net/article/35643/download/xml/
A reverse phase enantio-selective high performance liquid chromatographic method was developed for enantiomeric separation of 2-(1,3-dimethyl-2,6-dioxo-2,3-dihydro-1H-purine-7(6H)-yl)-N’-(3-fluorobenzylidene)-propanehydrazide isomers. The enantiomers of 2-(1,3-dimethyl-2,6-dioxo-2,3-dihydro-1H-purine-7(6H)-yl)-N’-(3-fluorobenzylidene)propanehydrazide were resolved on a ACE®Equivalence™ С18 (250 × 4.6 mm, 5 μm) column using a mobile phase system containing methanol, water, phosphate buffer рН 7.4 (50:46:4 v/v/v). The resolution between enantiomers was found to be more than 2.0. The sample solution and mobile phase were found to be stable for at least 48 h. The final optimised method was successfully applied to separate the (R)- and the (S)-enantiomers of 2-(1,3-dimethyl-2,6-dioxo-2,3-dihydro-1H-purine-7(6H)-yl)-N’-(3-fluorobenzylidene)-propanehydrazide and was proven to be reproducible and accurate.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
xanthine-hydrazone
isomers
chiral RP-HPLC
Development of a chiral RP-HPLC method for identification of stereomers of newly synthesised xanthine-based hydrazide-hydrazone compound
Research Article
10.3897/pharmacia.66.e35293
2019-06-06
pharmacia
The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
author
Mincheva, Irena
Medical University of Sofia, Sofia, Bulgaria
author
Zaharieva, Maya
https://orcid.org/0000-0001-8548-5649
Institute of Organic chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria
author
Batovska, Daniela
Medical University of Sofia, Sofia, Bulgaria
author
Najdenski, Hristo
The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
author
Ionkova, Iliana
The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
author
Kozuharova, Ekaterina
https://orcid.org/0000-0001-6795-9660
2019-06-06
2019-06-06
2019
Pharmacia
2603-557X
0428-0296
66
1
7-11
2019
10.3897/pharmacia.66.e35293
https://pharmacia.pensoft.net/article/35293/
https://pharmacia.pensoft.net/article/35293/download/pdf/
https://pharmacia.pensoft.net/article/35293/download/xml/
Potentilla reptans is widely used in traditional medicine as an astringent, for treating diarrhoea, haemorrhoids and for bleeding gums. A recent ethnobotanical study has reported on the anti-mastitis effects of the aerial parts of P. reptans decoction. The aim of the present study is to evaluate the antibacterial potential of extracts and fractions, obtained from aerial parts of P. reptans against three strains of Staphylococcus aureus. The observed MICs were within the range of 0.325 – 2.5 mg/ml. Studied extracts and their fractions exerted mostly bacteriostatic effect, with the n-hexane fraction of hydroethanolic extract being the most active (MIC 0.313 mg/ml against S. aureus ATCC 6538 P). However, further investigations are necessary to reveal the precise mode of action of P. reptans against mastitis.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Potentilla reptans
antibacterial activity
mastitis
traditional use
Antibacterial acticivity of extracts from Potentilla reptans L.
Research Article
10.3897/pharmacia.66.e35048
2019-06-06
pharmacia
National University of Pharmacy, Kharkiv, Ukraine
author
Podolsky, Illya
https://orcid.org/0000-0002-2368-7170
National University of Pharmacy, Kharkiv, Ukraine
author
Shtrygol', Sergiy
2019-06-06
2019-06-06
2019
Pharmacia
2603-557X
0428-0296
66
1
13-18
2019
10.3897/pharmacia.66.e35048
https://pharmacia.pensoft.net/article/35048/
https://pharmacia.pensoft.net/article/35048/download/pdf/
https://pharmacia.pensoft.net/article/35048/download/xml/
The object of the present study, 2-methyl-3-(phenylaminomethyl)-1H-quinolin-4-one (Atristamine), has been deeply studied as a promising antidepressant with the unique spectrum of additional neuropharmacological properties. Previously, the memory-enhancing effects of Atristamine have already been studied in the passive-avoidance test after scopolamine-induced amnesia in mice. Thus, the study of the effects of Atristamine on the spatial learning and memory in the Morris water maze under physiological conditions was the next logical step of our research.
According to the results obtained, Atristamine (100 mg/kg) has almost the same effect on the main markers of the memory-enhancing activity (the escape latency and distance moved) as Piracetam (300 mg/kg) and Phenibut (20 mg/kg) chosen as the well-studied and widely-used memory enhancers. The escape latency decreased in the Atristamine group by 3.2 times compared to the vehicle control group, whereas Piracetam and Phenibut caused a significant reduction of this indicator by 4.3 and 3.7 times, respectively. Moreover, the rats from the Atristamine group swam 5.1 times shorter distance to the platform in the probe trial compared to animals from the vehicle control group. The distance moved was 3-fold shorter in the Piracetam group and decreased by 5.2 times in the Phenibut group.
All drugs used in this study caused considerable changes of inter-quadrant preferences of animals. Based on the analysis of the inter-quadrant behaviour of rats, it has been found that there are considerable differences in search strategies associated, probably, with distinct mechanisms of the memory and learning enhancing action of the drugs used.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
2-methyl-3-(phenylaminomethyl)-1H-quinolin-4-one
Atristamine
the Morris water maze
the memory enhancing effect
The memory and learning enhancing effects of Atristamine
Research Article
10.3897/pharmacia.66.e35083
2019-06-07
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Drapak, Iryna
https://orcid.org/0000-0002-6830-2908
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Zimenkovsky, Borys
National University of Pharmacy, Kharkiv, Ukraine
author
Perekhoda, Lina
National University of Pharmacy, Kharkiv, Ukraine
author
Yeromina, Hanna
https://orcid.org/0000-0003-2986-3507
Kharkiv Medical Academy of Postgraduate Education, Kharkiv, Ukraine
author
Lipakova, Kateryna
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Demchuk, Inna
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Rakhimova, Maryna
2019-06-07
2019-06-07
2019
Pharmacia
2603-557X
0428-0296
66
1
33-40
2019
10.3897/pharmacia.66.e35083
https://pharmacia.pensoft.net/article/35083/
https://pharmacia.pensoft.net/article/35083/download/pdf/
https://pharmacia.pensoft.net/article/35083/download/xml/
Aim. The aim of study was to determine of the parameters of the molecular structure of new 1-[2-(R-phenylimino)-4-methyl-3-(3-[morpholine-4-yl]propyl)-2,3-dihydro-1,3-thiazol-5-yl]ethane-1-one derivatives and QSAR-analysis. The latter can be considered as the theoretical basis for de novo design of new potential antioxidants.
Materials and methods. 14 new derivatives of 1-[2-(R-phenylimino)-4-methyl-3-(3-[morpholine-4-yl] propyl)-2,3-dihydro-1,3-thiazol-5-yl]ethane-1-one were involved in the study and their antioxidant activities were evaluated. Hyper-Chem 7.59 and BuildQSAR software were used for calculation of molecular descriptors and building the QSAR-models.
Results. The calculation of number of molecular descriptors (electronic, steric, geometric, energy) was carried out for the tested compounds: 14 derivatives of 1-[2-(R-phenylimino)-4-methyl-3-(3-[morpholine-4-yl] propyl) -2,3-dihydro-1,3-thiazol-5-yl]ethane-1-one. For QSAR analysis, the compounds studied were divided into a training and test sample. The correlations between the antioxidant activity level and abovementioned molecular descriptors were shown in multivariate linear QSAR-model: Activity = ∑хіаі + bі, where xi – molecular descriptor. Based on the analysis of the obtained QSAR-models, it was found that antioxidant activity increases with decreasing of the area, molecular volume, lipophilicity, polarisation and increasing the magnitude of the dipole moment. The increase in the energy of the bonds, the energy of inter-nuclear interactions, the energy of the lower vacant molecular orbit and the reduction of the energy of hydration and energy of the higher vacant molecular orbitals also results in an increase in the antioxidant activity. The greatest effect of effective charges on atoms on the antioxidant activity was detected: the increase in the charge value on the morpholine cycle Oxygen and the decrease in the charge size on the Sulphur atom of the thiazole ring and the Oxygen atom of the acetyl group. QSAR models with better statistics were selected. QSAR models obtained are characterised by high predictive ability, determined both by internal and external validation and can be used for virtual screening of the antioxidant activity of substances of this class of compounds.
Conclusions. 1). The study of the structure–activity relationships for 1-[2-(R-phenylimino)-4-methyl-3-(3- [morpholine-4-yl]propyl)-2,3-dihydro-1,3-thiazol-5-yl]ethane-1-one derivatives were carried out. 2). QSAR analysis revealed the following: polarisation, dipole moment, lipophilicity, energy parameters as well as the size of the molecule and its branching possessed the most significant effect on antioxidant activity; the antioxidant activities of the compounds were increased with the increase in their hydrophilic and reductive properties; the molecules with small volume and surface area showed the higher level of antioxidant activity. 3). Obtained QSAR models are proposed for antioxidant activity prediction within the above-mentioned row of compounds and can be considered as a theoretical basis for de novo design of new potential antioxidants.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
antioxidant activity
molecular descriptors
1-[2-(R-phenylimino)-4-methyl-3-(3-[morpholine-4-yl]propyl)-2
3-dihydro-1
3-thiazol-5-yl]ethane-1-one derivatives
QSAR analysis
QSAR-analysis of 1-[2-(R-phenylimino)-4-methyl-3-(3-[morpholine-4-yl]propyl)-2,3-dihydro-1,3-thiazol-5-yl]ethane-1-one’s derivatives as potential antioxidants
Research Article
10.3897/pharmacia.66.e35022
2019-06-07
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Strus, Oksana
https://orcid.org/0000-0003-1638-1162
National University of Pharmacy, Kharkiv, Ukraine
author
Polovko, Nataliia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Yezerska, Oksana
2019-06-07
2019-06-07
2019
Pharmacia
2603-557X
0428-0296
66
1
19-25
2019
10.3897/pharmacia.66.e35022
https://pharmacia.pensoft.net/article/35022/
https://pharmacia.pensoft.net/article/35022/download/pdf/
https://pharmacia.pensoft.net/article/35022/download/xml/
The aim of the research was to substantiate the technological parameters of cream production with the sapropel extract and work out the technological scheme for its production. In this study, extract of sapropel from the Prybych deposit, emulsion base, containing corn oil, emulsifier No.1, cetylstearyl alcohol, nisin, euxyl K 100 and purified water were employed. To carry out the research, a set of methods to analyse colloidal and thermal stability was used. Rheological properties of the samples were determined. The method of microscopic analysis was carried out to analyse the stability of the emulsion system. It was proved experimentally that, when producing the cream, it is appropriate to use the phase inversion method, and the emulsification needs to be carried out at 5000 r/m for 20 min. Size and shape of the sample drops, obtained at 5000 r/m, were monodisperse and more uniform, most of which range from 2 to 3 microns that indicates the system stability. The following parameters of technological process were determined: mixing temperature conditions, speed of homogenisation and mixing time.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
apropel extract
o/w emulsion cream
emulsification methods
technology
Justification of technological parameters of the cream production with sapropel extract
Research Article
10.3897/pharmacia.66.e35131
2019-06-07
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Chaban, Taras
https://orcid.org/0000-0003-0618-275X
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Ogurtsov, Volodymyr
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Chaban, Ihor
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Myrko, Iryna
Medical College of Burgas University “Prof. Dr. Asen Zlatarov”, Burgas, Bulgaria
author
Harkov, Stefan
https://orcid.org/0000-0003-1012-849X
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Lelyukh, Maryan
https://orcid.org/0000-0002-8123-0988
2019-06-07
2019-06-07
2019
Pharmacia
2603-557X
0428-0296
66
1
27-32
2019
10.3897/pharmacia.66.e35131
https://pharmacia.pensoft.net/article/35131/
https://pharmacia.pensoft.net/article/35131/download/pdf/
https://pharmacia.pensoft.net/article/35131/download/xml/
Novel N3 substituted derivatives of 4-iminothiazolidine-2-one were synthesised under the reactions of [2+3]cyclocondensation, thionation and aminolysis. The functionalisation of 3-phenyl-4-imino-thiazolydine-2-one was carried out in its C5 position under condensation Knoevenagel and nitrosation reactions. The antioxidant activity of the synthesised compounds was evaluated in vitro by the method of their scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. When compared with existing antioxidants, some of our compounds were found to be more potent.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
antioxidant activity
4-iminothiazolidine-2-ones
synthesis
Synthesis of some new 4-iminothiazolidine-2-ones as possible antioxidants agents
Research Article
10.3897/pharmacia.66.e35139
2019-07-09
pharmacia
Medical University of Sofia, Sofia, Bulgaria
author
Mincheva, Irena
Wild Rodopi, Smolian, Bulgaria
author
Jordanova, Michaela
Medical University of Sofia, Sofia, Bulgaria
author
Benbassat, Niko
https://orcid.org/0000-0001-8876-2728
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria
author
Aneva, Ina
Medical University of Sofia, Sofia, Bulgaria
author
Kozuharova, Ekaterina
https://orcid.org/0000-0001-6795-9660
2019-07-09
2019-07-09
2019
Pharmacia
2603-557X
0428-0296
66
49-52
2019
Genus Micromeria.
M
Ancev
author
V
Velchev
author
1989
text
9). BAS Publ. House, Sofia
1989
356
362
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Aneva
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2016
2016
A
Assenov
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2016
2016
10.1016/j.lwt.2015.03.053
D
Dimitrov
author
2011
2011
2011
2011
Euro+Med PlantBase (2011) Euro+Med PlantBase. http://ww2.bgbm.org/euroPlusMed/PTaxonDetail.asp?NameId=36285&PTRefFk=8000000
L
Evstatieva
author
2009
2009
10.17660/ActaHortic.2012.955.10
L
Evstatieva
author
1998
1998
10.1002/cbdv.201200121
Chemical composition of the essential oils of three Micromeria species growing in Macedonia and Bulgaria.
E
Kostadinova
author
2007
text
Macedonian Journal of Chemistry and Chemical Engineering
2007
26
1
3
7
10.5053/ekoloji.2009.725
I
Lulcheva
author
2017
2017
10.1016/S0031-9422(01)00352-1
2002
2002
Medicinal Plants Act (2002) Promulgated, SG No. 29/7.04.2000, Amended, SG No. 23/1.03.2002, 91/25.09.2002.
Ethnobotany and exploitation potential of Oreganum vulgare L.
I
Mincheva
author
2016
text
in the Rhodopes, Bulgaria PharmacologyOnLine
2016
3
168
173
10.2478/v10089-012-0010-8
GC-MS metabolic profiling and free radical scavenging activity of Micromeria dalmatica.
M
Nikolova
author
2016
text
Biologica Nyssana
2016
7
2
159
165
Flavonoid compounds and antioxidant activity of bulgarian species of micromeria. Annuaire de l’Université de Sofia “St.
M
Nikolova
author
2017
text
Kliment Ohridski” Faculte de Biologie
2017
102
7
13
Red List of Bulgarian vascular plants.
A
Petrova
author
2009
text
Phytologia Balcanica
2009
15
1
63
94
Balkan endemics in the Bulgarian flora.
A
Petrova
author
2010
text
Phytologia Balcanica
2010
16
2
293
311
10.1002/cbdv.201100429
10.1007/s00606-005-0303-y
10.1016/0305-1978(91)90088-H
Estimation of the status of representative populations of Sideritis scardica Griseb. in the Rhodope Mts.
M
Yordanova
author
2000
text
Phytologia Balcanica
2000
6
1
43
57
10.3897/pharmacia.66.e35139
https://pharmacia.pensoft.net/article/35139/
https://pharmacia.pensoft.net/article/35139/download/pdf/
https://pharmacia.pensoft.net/article/35139/download/xml/
The present work summarises preliminary results of an ethnobotanical study in the Rhodope Mountains. The aim was to assess the traditional home use of medicinal plants for herbal tea by local people and to estimate the threats to the Balkan endemic Clinopodium dalmaticum. Semi-structured interviews with local informants from 15 localities in Central and Eastern Rhodope Mountains were performed in 2015 and 2018. As a result, the most used plant species were listed. The major source of plants was recorded as being gathered from the wild. The plants, known with the local names “wild mint” and “white mint”, were identified as Clinopodium dalmaticum from dried specimens presented by informants in Central Rhodopes. A frequent collection from wild populations of C. dalmaticum in Central Rhodope Mts. (Smoljan region) was revealed along with just a few cases of cultivation close to the studied sites.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
sustainable use
medicinal plants
plant collection
herbal tea
Clinopodium dalmaticum
Ethnobotany and exploitation of medicinal plants in the Rhodope Mountains – is there a hazard for Clinopodium dalmaticum?
Research Article
10.3897/pharmacia.66.e35023
2019-07-09
pharmacia
National University of Pharmacy, Kharkiv, Ukraine
author
Burda, Nadiia
https://orcid.org/0000-0002-7435-5731
National University of Pharmacy, Kharkiv, Ukraine
author
Zhuravel, Iryna
Al-Ahliyya Amman University, Amman, Jordan
author
Dababneh, Moeen F.
Nizhyn Gogol State University, Nizhyn , Ukraine
author
Fedchenkova, Yulia
https://orcid.org/0000-0003-1240-3053
2019-07-09
2019-07-09
2019
Pharmacia
2603-557X
0428-0296
66
41-44
2019
GC-MS Analysis of Tribulus terrestris.
P
Abirami
author
2011
text
Asian Journal of Plant Science and Research
2011
1
4
13
16
10.4103/0973-7847.125530
MV
Gavrilin
author
2008
Validatsiya analiticheskikh metodik.
2008
37 pp
10.1016/j.phytochem.2013.04.005
Pharmacological scientific evidence for the promise of Tribulus terrestris.
M
Jameel
author
2012
text
International Research Journal of Pharmacy
2012
3
5
403
406
Comparative and quantitative determination of quercetin and other flavonoids in North Indian populations of Tribulus terrestris Linn. by HPLC.
A
Kumar
author
2012
text
International Journal of Pharma and Bio Sciences
2012
3
4
69
79
Comparative, qualitative and quantitative chemotypic characterization among North Indian Tribulus terrestris.
A
Kumar
author
2012
text
International Research Journal of Pharmacy
2012
3
6
212
218
10.5923/j.ijmb.20120203.01
Spectroscopic determination of total phenolic and flavonoid contents of Tribulus terrestris fruits.
NB
Patil
author
2012
text
International Journal of ChemTech Research
2012
4
3
899
902
Pharmacognostical studies on Tribulus terrestris and Tribulus alatus.
M
Raja
author
2011
text
Der Pharmacia Sinica
2011
2
4
136
139
Comparative pharmacognostical and phytochemical evalution of Brihat and Laghu Gokshur.
K
Renuka
author
2012
text
Journal of Applied Pharmaceutical Science
2012
02
08
124
127
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10.5504/BBEQ.2011.0036
10.3390/molecules15020613
Comparative and quantitative determination of quercetin and rutin in Tribulus terrestris L. fruits from different seasonal and geographical populations of South India.
SR
Yanala
author
2018
text
Indo American Journal of Pharmaceutical Sciences
2018
05
03
1362
1367
10.3897/pharmacia.66.e35023
https://pharmacia.pensoft.net/article/35023/
https://pharmacia.pensoft.net/article/35023/download/pdf/
https://pharmacia.pensoft.net/article/35023/download/xml/
Caltrop (Tribulus terrestris L.) is a weed plant widely growing in warm climate. The crude caltrop has multidirectional pharmacological action favorable for the development of drug preparations. As in European countries caltrop is a non-officinal plant, it seems feasible to develop raw material standardization parameters for creation of novel drugs. In the course of thin layer chromatography research the following compounds were identified in caltrop herb purveyed during the fruiting season: rutin, chlorogenic acid, caffeic acid, diosgenin. Diosgenin content in this raw material was determined by densitometry to be min. 0,11%.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
caltrop
herb
TLC
densitometry
Analysis of diosgenin and phenol compounds in Tribulus terrestris L.
Research Article
10.3897/pharmacia.66.e35132
2019-07-09
pharmacia
Medical University of Pleven, Pleven, Bulgaria
author
Tzankov, Stanislav
Medical University – Sofia, Sofia, Bulgaria
author
Tzankov, Borislav
2019-07-09
2019-07-09
2019
Pharmacia
2603-557X
0428-0296
66
45-48
2019
10.1002/j.1875-9114.1984.tb03380.x
JG
Hardman
author
1992
1992
10.1136/openhrt-2015-000365
10.1002/j.1552-4604.1992.tb03811.x
Formulation and study of some controlled release tablets with pentoxifylline based on hydroxypropylcellulose matrix obtained by wet granulation method with PEG 6000.
E
Mircia
author
2010
text
Broad research in artificial intelligence and neuroscience
2010
1
2067
3957
10.1515/afpuc-2015-0026
Comparative pharmacokinetic analysis of novel sustained-release dosage forms of pentoxifylline in healthy subjects.
I
Raz
author
1998
text
nternational journal of clinical pharmacology, therapy, and toxicology
1998
26
206
208
10.3329/jsr.v1i2.1080
2011
Martindale
2011
Sweetman SC [Ed.] (2011) Martindale. Pharmaceutical Press, London, 4142 pp.
10.4172/jbb.1000018
2000
2000
United states pharmacopoeia XXIV-NF XIX, Asian edition (2000) USP convention Inc., 1441–1443.
10.1002/macp.1968.021180102
10.3897/pharmacia.66.e35132
https://pharmacia.pensoft.net/article/35132/
https://pharmacia.pensoft.net/article/35132/download/pdf/
https://pharmacia.pensoft.net/article/35132/download/xml/
The possibilities for correction of the rate of drug release by minimal changes in the technology process and by maintaining the quantitative and qualitative composition of the product pentoxifylline – extended release tablets were explored. Correction was made by addition of different quantity of HPMC (4000 cPs) to a granulating solution of PEG 6000. The main characteristics of the granules (compressibility index and density) were established. The swelling of the hydrogel tablets in water was determined in order more information on the release process to be obtained. Dissolution profiles of produced tablets were determined.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
pentoxifylline tablets
swelling index
dissolution profiles
Process optimization of preparation of pentoxifylline – extended release tablets
Research Article
10.3897/pharmacia.66.e35279
2019-07-09
pharmacia
Lviv Polytechnic National University, Lviv, Ukraine
author
Krychkovska, Aelita
Lviv Polytechnic National University, Lviv, Ukraine
author
Zayarnyuk, Nataliya
Lviv Polytechnic National University, Lviv, Ukraine
author
Lobur, Ivanna
Lviv Medical Institute, Lviv, Ukraine
author
Khomenko, Olena
Medical University, Lviv, Ukraine
Lviv Polytechnic National University, Lviv, Ukraine
author
Lopatynska, Oksana
https://orcid.org/0000-0002-6952-8001
Lviv Polytechnic National University, Lviv, Ukraine
author
Hubytska,, Iryna
Lviv Polytechnic National University, Lviv, Ukraine
author
Novikov, Volodymyr
2019-07-09
2019-07-09
2019
Pharmacia
2603-557X
0428-0296
66
59-65
2019
C
Gertosio
author
2016
2016
10.1016/S0140-6736(17)30552-4
A
Krychkovska
author
2018
2018
10.1080/20786204.2012.10874170
10.21691/fst.v10i1.73
10.3897/pharmacia.66.e35279
https://pharmacia.pensoft.net/article/35279/
https://pharmacia.pensoft.net/article/35279/download/pdf/
https://pharmacia.pensoft.net/article/35279/download/xml/
This article presents the results of the study of problematic issues of organisational-and-economic character concerning the provision of children with baby food products in Ukraine and European Union countries. It is established that the marketing feature of baby food products in Ukraine is its sales from pharmacies. It has been proved that the cost of baby food products in EU countries and in Ukraine have no price gradation depending on the degree of economic development of the country. The dynamics of development of the Ukrainian market of baby food products, as well as the dynamics of the cost of baby food products in Ukraine, depending on their dispensing place, have been analysed. It is established that the cost of baby food products is somewhat lower in pharmacies.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
nutrition support
neonatal period
infant nutrition
baby food market
Organisational and economic characteristics of the baby food products market in Ukraine and EU countries
Research Article
10.3897/pharmacia.66.e35162
2019-07-09
pharmacia
Medical University of Sofia, Sofia, Bulgaria
author
Tachkov, Konstantin
https://orcid.org/0000-0002-3961-7556
Medical University of Sofia, Sofia, Bulgaria
author
Mitov, Konstantin
Medical University of Sofia, Sofia, Bulgaria
author
Savova, Alexandra
2019-07-09
2019-07-09
2019
Pharmacia
2603-557X
0428-0296
66
53-57
2019
Evaluation of COPD treatment patterns for pulmonologists in Bulgaria.
K
Andreevska
author
2010a
text
Trakia Journal of Sciences
2010a
8
2
358
364
Medico social analysis of the prevalence of COPD.
К
Andreevska
author
2010b
text
Scripta Periodica
2010b
13
2
9
20
10.2147/COPD.S150243
J
Bousqet
author
2007
Global Surveillance, Prevention and Control of Chronic Respiratory Diseases, A Comprehensive Approach.
2007
155 pp
Cost-effectiveness of interventions for COPD using Ontario Policy Model.
K
Chandra
author
2012
text
Ont Health Technol Assess Ser
2012
12
12
1
61
10.1080/13696998.2017.1279620
10.1183/13993003.congress-2015.PA1053
10.2165/11591340-000000000-00000
2015
2015
Ministry of Health (2015) Yearly report on the health condition of the citizens of Republic Bulgarian and fulfillment of the health strategy. http://ncphp.government.bg/files/doklad_zdrave_-17-10.pdf
CJL
Murray
author
1996
1996
New epidemiological data for COPD in Pleven region.
P
Pavlov
author
2012
text
Thoracic Medicine
2012
4
2
44
50
10.1164/ajrccm.161.2.9901044
10.1080/13102818.2017.1335616
10.4066/biomedicalresearch.29-18-938
10.1016/j.jval.2018.09.2409
10.1001/jama.291.21.2616
10.3897/pharmacia.66.e35162
https://pharmacia.pensoft.net/article/35162/
https://pharmacia.pensoft.net/article/35162/download/pdf/
https://pharmacia.pensoft.net/article/35162/download/xml/
The aim was to estimate the economic burden, as well as rate of progression of COPD for a cohort of 426 patients for a 10-year period. A total of 426 patients from 19 regions with Chronic Obstructive Pulmonary Disease were enrolled in a representative, ambispective, national study for Bulgaria. Patients were recorded on disease stage, occupation, smoking habits and medication. Cost of treatment was calculated and a 10-year one-way Markov model was used by employing transition probabilities and quality of life data from available literature. Costs and outcomes were recorded and a cost-effectiveness acceptability curve (CEAC) was established.
Out of all patients included in the study 288 were non-smokers with a mortality percentage after 10 years of 42.7%. Smokers showed faster transition rate with 139 of them transitioning to more severe states and mortality was 54.6%. The incremental cost-effectiveness ratio (ICER) was 863.75 BGN. Patients who smoked had a lower quality of life – cumulatively for the 10-year period QALY = 623.51 for smokers vs. 1557.51 for non-smokers, but also lower costs for treatment (538 007.52 BGN vs. 1 344 757.95 BGN) accounted by the high transition rate and mortality amongst that group. Relatively few non-smokers transitioned from a less severe to a more severe state (6%), while most of the very severe stage patients experienced a lethal outcome within 10 years (81%).
Despite the higher costs associated with the disease, the Quality of Life and lower transition probability would enable patients to live a normal life. The ICER was well below the WHO threshold.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
COPD
Markov Model
Pharmacotherapy
Cost-effectiveness
Predicting the outcomes and costs for a cohort of 426 patients with Chronic Obstructive Pulmonary Disease (COPD) in Bulgaria through a Markov model
Research Article
10.3897/pharmacia.66.e36530
2019-07-23
pharmacia
Bulgarian Pharmaceutical Union, Sofia, Bulgaria
author
Balkanski, Stefan
https://orcid.org/0000-0002-3193-7918
Мedical University, Pleven, Bulgaria
author
Simeonova, Joana
Bulgarian Pharmaceutical Union, Sofia, Bulgaria
author
Gitev, Ivan
Мedical University, Sofia, Bulgaria
Bulgarian Pharmaceutical Union, Sofia, Bulgaria
author
Getov, Ilko N.
2019-07-23
2019-07-23
2019
Pharmacia
2603-557X
0428-0296
66
79-83
2019
10.18553/jmcp.2014.20.12.1162
10.1136/bmjopen-2015-009739
10.1157/13125364
10.1377/hlthaff.21.5.182
Guidelines on good pharmacy practice: Standards for quality of pharmacy services. WHO Technical Report, no.
2011
text
2011
961
310
23
CA
Gaither
author
2015
2015
Testing willingness to pay for blood pressure measurement in community pharmacy.
EE
Grigorov
author
2012
text
African Journal of Pharmacy and Pharmacology
2012
6
13
1005
1010
10.1016/j.sapharm.2010.12.001
2018
2018
IPU (2018) IPU pilot to detect hypertension and atrial fibrillation in the community 2018.
SB
Kayne
author
2005
2005
10.1016/j.socscimed.2010.04.021
10.1016/j.sapharm.2013.02.005
10.1016/j.sapharm.2018.01.010
2018
2018
PGEU (2018) PGEU Annual Report 2018. https://www.pgeu.eu/publications/annual-report/
10.1136/bmj.c2298
10.3390/ijerph7083150
10.1001/archinternmed.2011.399
10.2165/00019053-200725090-00006
2007
2007
TheDrugAct (2007) Law for medicinal products in human medicine. Http://Lex.Bg/Bg/Laws/Ldoc/2135549536
10.1111/ijpp.12037
Vaccination Antigrippale: Se Préparer à La Généralisation. La Revue Trimestrielle de l’Ordre National Des Pharmaciens, no.
C
Wolf-Thal
author
2019
text
2019
9
6
7
D
Wright
author
2016
2016
10.3897/pharmacia.66.e36530
https://pharmacia.pensoft.net/article/36530/
https://pharmacia.pensoft.net/article/36530/download/pdf/
https://pharmacia.pensoft.net/article/36530/download/xml/
Value-added pharmacy services (VAPS) are additional services to the traditional pharmacy activities, which do not include dispensing of medicinal products and professional consultation. Over 51% of the community pharmacies in Bulgaria offer VAPS but mainly measuring of blood pressure (67.4%) and blood glucose (12.9%). About 60% of community pharmacists in the country are willing to perform other VAPS. About 70% of them believe that patients would rate VAPS as useful. Younger pharmacists with professional experience less than 5 years (90.9%) tend to believe that VAPS would be positively rated by their patients. The study shows that VAPS different from consultation and dispensing of medicinal products have a potential for development in the community pharmacies in Bulgaria.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Value-added pharmacy services
pharmacists
community pharmacy
patient benefits
Evaluation of the current status of the value-added pharmacy services and pharmacists’ attitude in Bulgaria
Research Article
10.3897/pharmacia.66.e35976
2019-07-23
pharmacia
Medical University, Sofia, Bulgaria
author
Kostadinova, Ivanka
https://orcid.org/0000-0002-7283-8983
Medical University, Sofia, Bulgaria
author
Danchev, Nikolai
2019-07-23
2019-07-23
2019
Pharmacia
2603-557X
0428-0296
66
67-74
2019
10.1172/JCI111331
N
Angelova
author
2013
Farmacia – dela i dokumenti.
2013
116 pp
10.1517/13543780903002082
10.1016/j.clinthera.2009.02.004
10.1113/jphysiol.1981.sp013666
10.1113/jphysiol.1982.sp014180
10.3109/21556660.2013.833099
10.3109/21556660.2013.791623
10.1016/S0140-6736(08)61620-7
10.1016/j.clinthera.2012.03.007
10.1016/0014-2999(88)90343-3
10.1016/0013-4694(70)90164-1
10.1002/ana.410270215
10.1016/S0035-3787(04)71001-2
10.1016/j.clim.2011.06.004
10.1016/j.tins.2008.04.001
10.1016/S0022-510X(98)00143-9
10.1523/JNEUROSCI.19-20-08789.1999
10.1212/01.wnl.0000326213.89576.0e
10.1007/s13311-012-0156-3
10.1111/j.1365-2796.1989.tb01387.x
10.1111/j.1527-3458.2004.tb00029.x
10.1177/0091270003251388
10.1016/j.pharmthera.2005.10.006
Modulation of outward K+ conductance is a post-activational event in rat T lymphocytes responsible for the adoptive transfer of experimental allergic encephalomyelitis.
S
Judge
author
1997
text
Journal of Biomedical Science
1997
4
98
110
10.1002/mus.880030203
10.1152/jn.1992.67.1.125
10.1016/S0028-3908(02)00339-8
10.1097/00000542-197905000-00008
V
Mitzov
author
1967
1967
10.1007/978-3-642-70682-0_33
10.1097/00002060-199202000-00005
10.1111/j.1753-5174.2009.00021.x
10.1093/brain/123.1.171
10.1098/rstb.1999.0510
10.1002/14651858.CD001330
10.1016/S0079-6123(08)00676-6
10.1111/j.1753-5174.2009.00027.x
10.1113/jphysiol.1995.sp021036
10.1056/NEJM198206243062505
S
Waxman
author
1996
1996
10.1056/NEJM199801293380610
Potassium channels as therapeutic targets for autoimmune disorders.
H
Wulff
author
2003
text
Current Opinion in Drug Discovery & Development
2003
6
640
647
10.3897/pharmacia.66.e35976
https://pharmacia.pensoft.net/article/35976/
https://pharmacia.pensoft.net/article/35976/download/pdf/
https://pharmacia.pensoft.net/article/35976/download/xml/
In this review are described the preclinical and clinical pharmacological data as well as new therapeutic indications for the use of 4-aminopyridine. 4-aminopyridine is a potassium (K+) channel blocker that has a long history and various application areas. It is a chemical agent developed in 1963 as a bird poison. The first approval for clinical application of 4-aminopyridine was in 70’s in Bulgaria, since anesthetists in that country have confirmed its effect as reversal agent for nondepolarizing myorelaxants. The Bulgarian pharmaceutical company Sopharma commersialized 4-aminopyridine under the trade name Pymadin. Since then 4-aminopyridine was extensively studied and in 2010 is approved in the USA for the treatment of walking disabilities in patients with multiple sclerosis. In recent years, data from clinical trials indicated that K-channel blockade may prove to be an appropriate strategy to overcome disturbances in nerve impulses conduction associated with demyelination of the central nervous system.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
4-aminopyridine
multiple sclerosis
neurodegenerative diseases
4-aminopyridine – the new old drug for the treatment of neurodegenerative diseases
Research Article
10.3897/pharmacia.66.e36143
2019-07-23
pharmacia
Medical University, Plovdiv, Bulgaria
author
Becheva, Maria
https://orcid.org/0000-0002-2734-8280
Medical University, Plovdiv, Bulgaria
author
Petrova, Gergana
Medical University, Plovdiv, Bulgaria
author
Kirkova-Bogdanova, Angelina
https://orcid.org/0000-0002-9884-8186
UMHATEM “N. I. Pirogov”, Sofia, Bulgaria
author
Atanasov, Petar
2019-07-23
2019-07-23
2019
Pharmacia
2603-557X
0428-0296
66
75-77
2019
M
Becheva
author
2015
2015
Y
Bergeron
author
2008
2008
M
Dufur
author
1999
1999
10.1016/S1779-0123(08)70028-7
R
Kostov
author
2010
2010
T
Krastev
author
2016
Kinesitherapy in functional disorders of the sacroiliac joint.
2016
26 pp
G
Pierron
author
2000
2000
N
Popov
author
2009
2009
N
Popov
author
2013
2013
10.1016/j.berh.2009.08.013
10.1016/j.lpm.2009.11.022
10.1016/j.kine.2012.02.002
10.1136/ard.2004.028886
10.1016/j.joca.2007.12.013
10.3897/pharmacia.66.e36143
https://pharmacia.pensoft.net/article/36143/
https://pharmacia.pensoft.net/article/36143/download/pdf/
https://pharmacia.pensoft.net/article/36143/download/xml/
Hip arthrosis is the main cause of disability of all degenerative joint diseases and therefore it is considered to be of social significance. The presented clinical case aims to confirm the efficacy of the applied kinesitherapeutic program in conservative treatment of 62-year-old patient with right-sided coxarthrosis. The kinesitherapeutic methods included right lower limb massage, suspension therapy, tractions and manual movements of the right hip joint, positional treatment, active exercisesinan openkinetic chain, postisometric relaxation and stretching for shortened muscles. The pain symptoms were reduced, the volume of movement in the right hip increased, the strength of the right hip joint stabilizer muscles partially improved.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
coxarthrosis
functional deficiency
kinesitherapy
Kinesitherapeutic behaviour in conservative treatment of coxarthrosis – case report
Research Article
10.3897/pharmacia.66.e37035
2019-10-10
pharmacia
Faculty of Pharmacy, Medical University - Sofia, Sofia, Bulgaria
author
Ilieva, Viktoria
https://orcid.org/0000-0001-7428-602X
Faculty of Pharmacy, Medical University - Sofia, Sofia, Bulgaria
author
Kondeva-Burdina, Magdalena
National Center of Public Health and Analyses, Sofia, Bulgaria
author
Georgieva, Tzveta
https://orcid.org/0000-0002-3066-9448
National Center of Public Health and Analyses, Sofia, Bulgaria
author
Pavlova, Vera
2019-10-10
2019-10-10
2019
Pharmacia
2603-557X
0428-0296
66
91-97
2019
10.3897/pharmacia.66.e37035
https://pharmacia.pensoft.net/article/37035/
https://pharmacia.pensoft.net/article/37035/download/pdf/
https://pharmacia.pensoft.net/article/37035/download/xml/
As a result of blooming, some cyanobacteria (Microcystis, Anabaena, Planktothrix, etc.) produce toxins at concentrations that are high enough to poison and even kill animals and humans. According to Annex II of the Framework Directive 2000/60/EC, transformed into the Bulgarian legislation, for the characterization of surface water, at this stage, there are no acceptable limit values for cyanotoxins. Cyanotoxins include some of the strongest natural poisons, including those that could cause rapid death due to respiratory failure.
The aim of this publication is an overview of toxic cyanobacteria and toxicity mechanisms.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
cyanobacteria
mechanism of action
toxicity
Toxicity of cyanobacteria. Organotropy of cyanotoxins and toxicodynamics of cyanotoxins by species
Review Article
10.3897/pharmacia.66.e37263
2019-10-10
pharmacia
author
Mitkov, Javor
Faculty of Pharmacy, Medical University - Sofia, Sofia, Bulgaria
author
Kondeva-Burdina, Magdalena
Faculty of Pharmacy, Medical University - Sofia, Sofia, Bulgaria
author
Zlatkov, Alexander
https://orcid.org/0000-0002-1695-8454
2019-10-10
2019-10-10
2019
Pharmacia
2603-557X
0428-0296
66
99-106
2019
10.3897/pharmacia.66.e37263
https://pharmacia.pensoft.net/article/37263/
https://pharmacia.pensoft.net/article/37263/download/pdf/
https://pharmacia.pensoft.net/article/37263/download/xml/
New series of caffeine-8-(2-thio)-propanoic hydrazid-hydrazone derivatives were designed and synthesized. The targed compounds were obtained in yields of 51 to 96% and their structures were elucidated by FTIR, 1H NMR, 13C NMR, MS and microanalyses. All of the compounds were found to be “drug-like” as they fulfill the criteria of drug-likeness, which includes the MDDR-like rule. The tested compounds were subjected to in silico prediction of substrate/metabolite specificity and Drug Induced Liver Injury (DILI). The prediction for indicated that the evaluated compounds would most probably act as CYP1A2 substrates. The performed in vitro studies didn’t reveal statistically significant hepatotoxicity of the tested compounds, probably due to the pro-oxidant effects expressed on sub-cellular (isolated rat liver microsomes) level. The obtained experimental results confirmed the predicted low hepatotoxicity for the tested structures. Based on these results the compounds may be considered as promising structures for design of future molecules with low hepatotoxicity.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
caffeine
isolated rat liver microsomes
pro-oxidant effects
Synthesis and preliminary hepatotoxicity evaluation of new caffeine-8-(2-thio)-propanoic hydrazid-hydrazone derivatives
Research Article
10.3897/pharmacia.66.e35880
2019-10-10
pharmacia
Medical University-Sofia, Faculty of public health, Sofia , Bulgaria
author
Stoimenova, Assena
Bulgarian Drug Agency , Sofia , Bulgaria
author
Kirilov, Bogdan
Medical University-Varna, Faculty of medicine, Sofia , Bulgaria
author
Zaykova, Krassimira
2019-10-10
2019-10-10
2019
Pharmacia
2603-557X
0428-0296
66
85-89
2019
10.3897/pharmacia.66.e35880
https://pharmacia.pensoft.net/article/35880/
https://pharmacia.pensoft.net/article/35880/download/pdf/
https://pharmacia.pensoft.net/article/35880/download/xml/
The current study analyses the regulatory inspection findings of the wholesalers in Bulgaria in 2017 and compares the results with the findings from some other EU member-states. In total, 48 GDP inspections were performed in 2017. 50% of the inspections were performed in relation with issuing an authorization for wholesale of medicines, the rest half were related to changes in already granted authorizations.
During the inspections, 17 non-conformities (NCs) have been documented. The NCs were identified in 3 wholesalers and 6 deficiencies were classified as major. No critical deficiencies were found. NCs were found in 6.25% of the inspected companies. No critical NCs were identified and only 6 NCs were classified as major which demonstrated high level of compliance of distributions sites in Bulgaria with the requirements of GDP.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Good Distribution Practice (GDP)
medicinal products
non-conformities
deficiencies
supply chain
compliance
Bulgarian Drug Agency
Analysis of good distribution practice inspection deficiency data of pharmaceutical wholesalers in Bulgaria
Research Article
10.3897/pharmacia.66.e38573
2019-10-10
pharmacia
Faculty of Pharmacy, Medical University - Sofia, Sofia, Bulgaria
author
Yordanov, Yordan
https://orcid.org/0000-0003-1029-9870
2019-10-10
2019-10-10
2019
Pharmacia
2603-557X
0428-0296
66
107-114
2019
10.3897/pharmacia.66.e38573
https://pharmacia.pensoft.net/article/38573/
https://pharmacia.pensoft.net/article/38573/download/pdf/
https://pharmacia.pensoft.net/article/38573/download/xml/
Caffeic acid phenethyl ester (CAPE) is the major pharmacologically-active component of some propolis types, rich in polyphenols, such as poplar propolis types. CAPE has the potential to be applied as a pharmaceutical as it possesses most of the pharmacological activities of propolis, such as anti-proliferative, antioxidant, immunomodulatory, antidiabetic, anti-inflammatory and antimicrobial. Its advantage is that it lacks some of the downsides of total propolis extracts, such as inability for unified standardization, which is cornerstone for implementing its therapeutic potential as a drug. The current paper provides an overview on the pharmacodynamic principles of CAPE. We present literature search outcomes form ClinicalTrials.gov database and from scientific publications, available on Scopus and Crossref databases. We take a round view of CAPE’s potential therapeutic implications in light of approved drugs with related modes of action.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Pharmacology
therapeutic application
selective inhibitor of nuclear export
NF-κB activation inhibition
Caffeic acid phenethyl ester (CAPE): pharmacodynamics and potential for therapeutic application
Review Article
10.3897/pharmacia.66.e35969
2019-11-07
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Hudz, Nataliia
https://orcid.org/0000-0002-2240-0852
Ukrainian Scientific Pharmacopoeial Center for Quality of Medicines, Kharkiv, Ukraine
author
leontiev, Dmytro
University of Opole, Opole, Poland
author
Wieczorek, Piotr P.
2019-11-07
2019-11-07
2019
Pharmacia
2603-557X
0428-0296
66
121-125
2019
10.3897/pharmacia.66.e35969
https://pharmacia.pensoft.net/article/35969/
https://pharmacia.pensoft.net/article/35969/download/pdf/
https://pharmacia.pensoft.net/article/35969/download/xml/
Objectives: To study 5-hydroxymethylfurfural (5-HMF) spectral characteristics aiming at their future application in analytical procedures and their validation for the determination of 5-HMF in liquid products containing glucose after sterilization. Method: Direct spectrophotometric method for the determination of 5-HMF using the molar absorption coefficient at the absorption maximum (284 nm).
Results and discussion: aqueous 5-HMF solutions have strong absorption in the ultraviolet range below 310 nm and give two absorption maxima at wavelengths of 229–230 nm and 284 nm. An excellent linear relationship between absorbance and 5-HMF concentration was observed in the concentration range of 2.0–10.0 mg/l. The linear dependence passes through the origin. The molar absorption coefficients of 5-HMF were determined and found to be 3007 mol−1•L•cm−1at 229–230 nm and 16070 mol−1•L•cm−1at 284 nm. The use of the molar absorption coefficient of 5-HMF stated in the Pharmacopeia of the United States of America for determining 5-HMF in polydextrose (16830 mol−1·L·cm−1at 283 nm) gives recovery results for model solutions of reference substance of 5-HMF that are acceptable from the point of view of the requirements of the State Pharmacopeia of Ukraine for methods of quantitative determination of impurities. However, other values of the molar absorption coefficient (17000 and 22700 mol−1•L•cm−1) given in the scientific publications are unsuitable for the quantitative determination of 5-HMF as an impurity in medicinal products.
Conclusion: The molar absorption coefficient (16830 mol−1•L•cm−1at 284 nm) may be used to quantify 5-HMF as an impurity in medicinal products containing glucose. For a specific medicinal product, a full validation of the analytical procedure of the 5-HMF determination is required taking into account the composition of this product.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
5-hydroxymethylfurfural
spectrophotometric method
molar absorption coefficient
medicinal products containing glucose
Spectral characteristics of 5-hydroxymethylfurfural as a related substance in medicinal products containing glucose
Short Communication
10.3897/pharmacia.66.e37954
2019-11-07
pharmacia
Medical University – Sofia, Sofia, Bulgaria
author
Petkova, Valentina
https://orcid.org/0000-0002-6938-1054
Medical University – Plovdiv, Plovdiv, Bulgaria
author
Hadzhieva, Bozhidarka
https://orcid.org/0000-0002-8675-5695
Medical University – Sofia, Sofia, Bulgaria
author
Nedialkov, Paraskev
https://orcid.org/0000-0001-5640-6120
2019-11-07
2019-11-07
2019
Pharmacia
2603-557X
0428-0296
66
115-119
2019
10.3897/pharmacia.66.e37954
https://pharmacia.pensoft.net/article/37954/
https://pharmacia.pensoft.net/article/37954/download/pdf/
https://pharmacia.pensoft.net/article/37954/download/xml/
Medicinal plants, their extracts and herbal medicinal products occupy a growing share of medicines in the pharmacy worldwide.
Historically, the first medicines were products of plants, as well as some of the most important medicines still in use today. With the development of phytochemistry, as part of the pharmaceutical science, great progress has been made in the isolation and in determining the value of a number of biologically active substances (BAS).
Many plants have yielded pure substances (or natural products) that are applied in modern medical practice. Other compounds are potentially useful or have toxic effects.
Traditional medicine incorporating many herbal medicines remains an important (and in some cases, the only) form of treatment in some countries, with increasing use in medical practice.
On the other hand, the fact that in pediatric patients there is a limitation, mainly moral and ethical, of the number of medications to be administered due to the difficulty of conducting clinical trials in children, stimulates the use of herbal medicines of proven quality, effectiveness and safety among this group of patients.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
phytotherapeutic
alternative medicines
pediatric
medicinal plants
Phytotherapeutic approaches to treatment and prophylaxis in pediatric practice
Research Article
10.3897/pharmacia.66.e47035
2019-11-12
pharmacia
Medical University, Sofia, Bulgaria
author
Tzankova, Diava
Medical University, Sofia, Bulgaria
author
Peikova, Lily
https://orcid.org/0000-0002-8030-3479
University of Chemical Technology and Metallurgy, Sofia, Bulgaria
author
Vladimirova, Stanislava
https://orcid.org/0000-0002-2190-9980
Medical University, Sofia, Bulgaria
author
Georgieva, Maya
2019-11-12
2019-11-12
2019
Pharmacia
2603-557X
0428-0296
66
127-134
2019
10.1016/S0149-6395(01)80006-4
10.1055/s-0031-1296457
10.1016/j.bmc.2009.10.064
10.2174/157018010791306588
10.1016/S0223-5234(00)00123-9
10.2174/15734064113096660069
Determination of Hydrolytic stability and acute toxicity of previously synthesized pyrrole-based hydrazones.
M
Georgieva
author
2012
text
Pharmacia
2012
59
1
10
16
10.12955/cbup.v5.1075
10.1021/ac981022v
10.1155/2014/761030
10.1002/anie.200802651
10.1016/j.ejmech.2015.09.016
10.1016/j.jpba.2005.06.021
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10.2174/1573406411208030462
10.3390/molecules16010016
10.1016/j.bmc.2011.05.056
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10.1007/BF01129203
10.1093/chrsci/49.10.780
10.12955/cbup.v6.1314
10.12955/cbup.v6.1314
D
Tzankova
author
2019a
2019a
D
Tzankova
author
2019b
2019b
10.3109/14756366.2012.688043
10.15761/PDDT.1000103
10.1016/j.ejmech.2006.08.010
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10.3897/pharmacia.66.e47035
https://pharmacia.pensoft.net/article/47035/
https://pharmacia.pensoft.net/article/47035/download/pdf/
https://pharmacia.pensoft.net/article/47035/download/xml/
RP-HPLC method with UV detection was developed and validated for determination of the chemical stability and stability in close to physiological conditions of a model pyrrole hydrazone ethyl 5-(4-bromophenyl)-1-(1-(2-(4-hydroxy-3-methoxybenzylidene) hydrazineyl)-4-methyl-1-oxopentan-2-yl)-2-methyl-1H-pyrrole-3-carboxylate (D_5d), containing susceptible to hydrolysis hydrazone group. The evaluated substance was subjected to the influence of a variety of pH , representing the main physiological values of 37°C and corresponding pH values in the stomach (pH 2.0), blood (pH 7.4) and small intestine (pH 9.0). Chemical stability in a highly alkaline medium with a pH of 13.0 was also evaluated. The hydrazone I tested was found to be stable at pH 7.4 and pH 9.0 and 37 ° C and hydrolyzed under strong acidic (pH 2.0) and highly alkaline media (pH 13.0) and at the same temperature.The products of hydrolysis were identified to be the initial hydrazide and aldehyde, pointing the hydrazone group as most liable.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
pyrrole-hydrazide
pyrrole-hydrazone
stability
RP-HPLC
validation
Development and validation of RP-HPLC method for stability evaluation of model hydrazone, containing a pyrrole ring
Research Article
10.3897/pharmacia.66.e35075
2019-11-26
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Ryvak, Tetiana
https://orcid.org/0000-0002-9491-1109
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Denysiuk, Oksana
2019-11-26
2019-11-26
2019
Pharmacia
2603-557X
0428-0296
66
135-140
2019
10.18549/PharmPract.2016.03.782
Modern approaches towards issues concerning a healthy way of life.
SV
Cherevko
author
2013
text
Pedagogy Formation of Creative Personality in Higherand Secondary Schools
2013
33
86
409
414
Ye
Dmitrik
author
2015
2015
Ye
Dmitrik
author
2016
2016
SS
Harkavenko
author
2004
Marketing: a textbook.
2004
712 pp
BP
Hromovyk
author
2008
2008
Legal regulation of medicines advertising in Ukraine: problems and perspectives.
HV
Huseva
author
2014
text
Bulletin of the Academy of Advocacy of Ukraine
2014
2
30
40
48
Yu
Kordubaylo
author
2014
2014
N
Movchazna
author
2016
2016
The relevance of advertisemen to fmedical preparations on television.
I
Nosenko
author
2015
text
Scientific Research of Ukrainian Media Content: Social Dimension
2015
3
54
60
10.7324/JAPS.2017.70422
2018
2018
Ukrainian State List of Registered Drugs (2018) Ukrainian State List of Registered Drugs. [In Ukrainian] http://www.drlz.com.ua
2008
2008
World Self-Medication Industry, WSMI (2008) Advertising of non prescription medicines to the public. A significant contributor to health care. http://www.wsmi.org/wp-content/data/pdf/wsmi_brochureadvertising.pdf
2019
2019
World Self-Medication Industry, WSMI (2019) Advertising of non prescription medicines. http://www.wsmi.org/policy-principles/advertising-of-nonprescription-medicines/
Attitude of medical students for taking drugs: problem of irresponsible self-treatment.
N
Zaremba
author
2015
text
Pharmacia
2015
62
3
10
14
IA
Zupanets
author
2003
Pharmaceutical care: a course for lecturers for pharmacist sand family physicians.
2003
608 pp
10.3897/pharmacia.66.e35075
https://pharmacia.pensoft.net/article/35075/
https://pharmacia.pensoft.net/article/35075/download/pdf/
https://pharmacia.pensoft.net/article/35075/download/xml/
An assessment of the opinion of a certain cohort of the population on the role of drug advertising in the choice of non-prescription drugs for self-treatment was conducted. According to the questionnaire survey, it has been established that advertising affects the choice of drug for self-treatment 26.0% of the polled. Despite the fact that the majority of respondents indicate that there is no influence of drug advertising, 62.8% of them buy self-medication drug sabout which they learned from advertising. Thus, our scientific hypothesis about the presence of the direct influence of drug advertising on the commitment of patients to pharmacotherapy is confirmed. The majority of respondents (90.0%) do not consider advertising of drugs to be effective and safe, and 61.0% say that drug advertising contains contradictory and incomprehensible information. Almost all respondents (88.0%) are convinced that drug advertising is not always objective and fair. At the same time, according to 50.0% of respondents, the volume of drug advertising in Ukraine should be reduced, 22.0% of respondents believe that advertising of drugs should be prohibited.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
drug advertising
non-prescription drugs
self-treatment
questionnaire survey
Public opinion study on the role of advertising in choice of non-prescription drugs for self-treatment
Research Article
10.3897/pharmacia.66.e37739
2019-12-19
pharmacia
Saitama University, Saitama, Japan
University of Mining and Geology, Sofia, Bulgaria
author
Loukanov, Alexandre
https://orcid.org/0000-0002-5029-7354
Medical University-Sofia, University, Sofia, Bulgaria
author
Nikolova, Svetla
University of Forestry, Sofia, Bulgaria
author
Filipov, Chavdar
Saitama University, Saitama, Japan
author
Nakabayashi, Seiichiro
2019-12-19
2019-12-19
2019
Pharmacia
2603-557X
0428-0296
66
147-156
2019
10.3897/pharmacia.66.e37739
https://pharmacia.pensoft.net/article/37739/
https://pharmacia.pensoft.net/article/37739/download/pdf/
https://pharmacia.pensoft.net/article/37739/download/xml/
The nanomaterials for cancer medication are already reality providing a wide range of new tools and possibilities, from earlier diagnostics and improved imaging to better, more efficient, and more targeted anticancer therapies. The purpose of this critical review is to focus on the current use of clinically approved nanoparticles for cancer theranostic, nanovaccines and delivery platforms for gene therapy. These include inorganic, metal and polymer nanoparticles, nanocrystals and varieties of drug delivery nanosystems (micelles, liposomes, microcapsules and etc.). The recent progress in cancer nanomedicine enables to combine the benefits of individual nanoparticles with biomolecules into a multifunction nanomachines and even highly advanced nanorobots for targeted therapies. Nowadays clinical trials with advanced anticancer nanomachines provide potential for more accurately and effective identification and destruction of the cancer cells present in the human body.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Drug delivery nanosystems
targeted nanotherapy
nanomachines
personalized nanomedicine
Nanomaterials for cancer medication: from individual nanoparticles toward nanomachines and nanorobots
Review Article
10.3897/pharmacia.66.e38137
2019-12-19
pharmacia
National University of Pharmacy, Kharkiv, Ukraine
author
Severina, Hanna I.
https://orcid.org/0000-0003-2894-9384
National Pirogov Memorial Medical University, Vinnytsya, Ukraine
author
Skupa, Olga O.
National Pirogov Memorial Medical University, Vinnytsya, Ukraine
author
Voloshchuk, Natalya I.
National University of Pharmacy, Kharkiv, Ukraine
author
Suleiman, Marharyta M.
National University of Pharmacy, Kharkiv, Ukraine
author
Georgiyants, Victoriya A.
2019-12-19
2019-12-19
2019
Pharmacia
2603-557X
0428-0296
66
141-146
2019
10.1016/j.ijbiomac.2012.04.012
10.1016/j.jsps.2013.12.016
10.1089/thy.2016.0652
10.1155/2013/176213
10.1159/000222775
10.1021/jm970443m
10.1002/jhet.1573
10.1016/0165-0173(89)90003-9
10.1134/S1070428016010188
10.1016/0014-2999(73)90134-9
Alkylation of 6-methyl-2-thiouracile ω-dibromalkanes.
AM
Kigundi
author
2007
text
Bulleten Volgogradskogo Nauchnogo Centra RAMN
2007
1
28
31
10.1002/cmdc.201700589
10.1016/0920-1211(96)00022-8
10.1016/j.ejps.2017.03.014
10.1111/epi.13764
10.1139/v84-004
AA
Ozerov
author
2010
2010
10.1016/j.ejmech.2010.12.009
10.1007/s11094-014-1011-0
10.7324/JAPS.2019.90202
10.1016/S0960-894X(99)00227-9
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N
Zenker
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Foye
author
1990
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Lea & Febiger, Philadelphia, London
1990
603
621
10.2174/1573406411309050010
10.3897/pharmacia.66.e38137
https://pharmacia.pensoft.net/article/38137/
https://pharmacia.pensoft.net/article/38137/download/pdf/
https://pharmacia.pensoft.net/article/38137/download/xml/
The alkylation of 6-methyl-2-thioxo-2,3-dihydro-1H-pyrimidine-4-one phenacyl bromides under different conditions was investigated. It was found that during the reaction in the medium of DMF/K2CO3 a mixture of 2-(2-aryl-2-oxoethyl)thio-6-methyl-pyrimidine-4(3H)-one and 3-hydroxy-3-aryl-7-methyl-2,3-dihydro-5H-thiazolo[3,2-a]pyrimidine-5-one was formed. The holding of the resulting mixture in the concentrated sulphuric acid leads to the formation of cyclization products - derivatives of 3-aryl-7-methyl-5H-thiazolo[3,2-a]pyrimidin-5-one with high yields. Individual S-alkylated derivatives – 2-(2-aryl-2-oxoethyl)thio-6-methyl-pyrimidine-4(3H)-one - were obtained by reacting in methanol in the presence of sodium methoxide. Pharmacological screening of synthesized compounds for anticonvulsant activity on the model of pentylenetetrazole seizures in rats was carried out and some regularity “structure-activity” was established.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
synthesis
pyrimidine
thiazolopyrimidine
anticonvulsant activity
Synthesis and anticonvulsant activity of 6-methyl-2-((2-oxo-2-arylethyl)thio)pyrimidin-4(3 H)-one derivatives and products of their cyclization
Research Article
10.3897/pharmacia.66.e35186
2019-12-19
pharmacia
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Bilyk, Iryna
https://orcid.org/0000-0002-4048-2820
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Fedyak, Iryna
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Ivaniulyk, Iryna
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Havryshchuk, Liubomyr
2019-12-19
2019-12-19
2019
Pharmacia
2603-557X
0428-0296
66
157-160
2019
10.3897/pharmacia.66.e35186
https://pharmacia.pensoft.net/article/35186/
https://pharmacia.pensoft.net/article/35186/download/pdf/
https://pharmacia.pensoft.net/article/35186/download/xml/
A comprehensive study for assessment the appointments rationality for children with meningococcal infectious (MI) in Ukraine in real clinical practice has been conducted. The doctor’s appointment sheets from 184 inpatients medical cards have been processed by the integral ABC / VEN / frequency analysis. In average, children were in the hospital (14±7) bed-days, they were granted 2908 appointments of 191 drugs by the INN. It has been established that medicine for MI etiotropic therapy took the third place in the appointments frequency. At the same time, the ABC / VEN-analysis results revealed a rational approach to pharmacotherapy.The total treatment cost was estimated at USD 25,173.59 (for one child – USD 136.81).Since currently in Ukraine inpatient children treatment is mainly provided by parents, further medical prescriptions improvement is needed in order to increase the efficiency and minimize the childrenMI therapy cost.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
ABC-analysis
children
frequency analysis
meningococcal disease
VEN-analysis
Clinical and economic estimation of children’s pharmacotherapy with meningococcial infections in Ukraine
Research Article
10.3897/pharmacia.66.e36620
2019-12-31
pharmacia
Medical University Sofia, Sofia, Bulgaria
author
Drenska, Maria
Medical University Sofia, Sofia, Bulgaria
author
Elitova, Savina
Acibadem City Clinic Cancer Center, Sofia, Bulgaria
author
Grigorova, Velina
Medical University Sofia, Sofia, Bulgaria
author
Naseva, Emilia
https://orcid.org/0000-0002-1282-8441
Medical University Sofia, Sofia, Bulgaria
author
Getov, Ilko N.
2019-12-31
2019-12-31
2019
Pharmacia
2603-557X
0428-0296
66
165-170
2019
10.1002/14651858
The pharmacological management of migraine, part 2: preventative therapy.
G
Demaagd
author
2008
text
Pharmacy and Therapeutics:
2008
33
8
480
487
K
Edwards
author
2016
2016
2009
2009
European Commission; European Medicines Agency (2009) A guideline on summary of product characteristics. https://www.ema.europa.eu/en/human-regulatory/marketing-authorisation/product-information/how-prepare-review-summary-product-characteristics#scientific-guidelines-with-smpc-recommendations-section
2014
2014
European Commission; European Medicines Agency (2014) Pharm655. Human Pharmaceutical Committee Meetings. https://ec.europa.eu/health/sites/health/files/files/committee/72meeting/pharm655.pdf
2017
2017
European Medical Agency & Heads of Medicines Agencies (2017) Guideline on good pharmacovigilance practices (GVP) Annex I – Definitions (Rev 4). https://www.ema.europa.eu/en/human-regulatory/post-authorisation/pharmacovigilance/good-pharmacovigilance-practices
2001
2001
European Parliament (2001) Directive 2001/83/EC of the European Parliament and the Council. Official Journal of the European Communities. https://eur-lex.europa.eu/legal-content/en/ALL/?uri=CELEX%3A32001L0083
2004
2004
European Parliament (2004) Regulation (EC) No 726/2004 of the European Parliament and of the Council of 31 March 2004 laying down Community procedures for the authorisation and supervision of medicinal products for human and veterinary use and establishing a European Medicines Agency. https://eur-lex.europa.eu/legal-content/EN/ALL/?uri=CELEX:32004R0726 [revised 2019]
10.5694/j.1326-5377.2006.tb00689.x
10.4103/0976-500X.130046
2016
2016
History of Drug Regulation in Bulgaria (2016) Official page of the Executive Agency for Medicines in the Republic of Bulgaria. https://bda.bg/index.php?option=com_content&view=article&id=53&Itemid=9&lang=bg
10.1016/j.jpain.2009.07.001
10.1111/j.1526-4610.2004.04121.x
10.1310/sci2102-166
10.1001/archneur.61.8.1254
10.1016/j.phrs.2010.04.006
10.1016/S0035-3787(06)75021-4
10.1007/s10309-012-0237-7
10.1016/S1474-4422(08)70050-4
M
Weda
author
2017
2017
10.3389/fneur.2014.00286
10.3897/pharmacia.66.e36620
https://pharmacia.pensoft.net/article/36620/
https://pharmacia.pensoft.net/article/36620/download/pdf/
https://pharmacia.pensoft.net/article/36620/download/xml/
Introduction: The off-label use of medicines is a common practice that covers a wide range of therapeutic areas in both, adults and children. So far, the extent of off-label use among neurology patients in Bulgaria has not been studied. The aim of this study is to provide data on the off-label use in neurology patients in Bulgaria and to contribute to planning actions by the European Commission and EMA to provide a harmonized guideline and to regulate the off-label use of medicines within the European Union.
Materials and methods: The data on prescriptions of 360 neurology outpatients, treated in a 1 – year period, were recorded and provided for analyses. The Summaries of Product Characteristics, were used as reference documents for assessment of prescriptions.
Results: The results from this study show that most neurology patients (63%) were exposed to off-label use. Most of the medicines prescribed off-label (90%), were used for a therapeutic indication, other than the one listed in the authorized product information. Meloxicam is found to be the most commonly prescribed off-label medicine. Other medicines, like trasadone, pentoxyfylline and fupentixol / melitracen were prescribed less frequently, but deserve special attention, as they were found to be used off-label to a very large extent, some of them in 100% of prescriptions. Half of the top 10 medications, most commonly used off-label in neurology, were found to be non-steroidal anti-inflammatory drugs.
Conclusion: The results reveal a big gap between the authorized medicines and the real medical needs. Further studies based on a larger number of medical centers are needed to establish more accurate data on off-label prescribing in neurology patients on a national level.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
off-label use
neurology
pharmacovigilance
Analysis of primary outpatient data for off-label use of medicines in neurology
Research Article
10.3897/pharmacia.66.e46687
2019-12-31
pharmacia
Medical University Sofia, Sofia, Bulgaria
author
Drenska, Maria
Medical University Sofia, Sofia, Bulgaria
author
Naseva, Emilia
https://orcid.org/0000-0002-1282-8441
Medical University Sofia, Sofia, Bulgaria
author
Getov, Ilko N.
2019-12-31
2019-12-31
2019
Pharmacia
2603-557X
0428-0296
66
217-221
2019
Unlicensed and off label use of some medicines in obstetric practice.
C
Bozhinova
author
2008
text
Obstetrics and Gynecology
2008
47
5
43
47
10.1093/annonc/mdm517
10.1136/adc.83.6.502
10.1515/amb-2017-0003
10.1007/s40267-019-00638-4
10.1186/s13023-018-0895-2
10.1007/s00228-004-0752-1
D
Jolly
author
2014
2014
J
Petrova
author
2014
2014
10.1016/j.anpedi.2013.10.004
“Off label” – use of some medicines. Part I.
M
Sirakov
author
2012a
text
Obstetrics and Gynecology
2012a
51
2
29
32
“Off label” use of some medicines. Part II. “Off label” use of misoprostol.
M
Sirakov
author
2012b
text
Obstetrics and Gynecology
2012b
51
3
50
54
M
Weda
author
2017
2017
10.3897/pharmacia.66.e46687
https://pharmacia.pensoft.net/article/46687/
https://pharmacia.pensoft.net/article/46687/download/pdf/
https://pharmacia.pensoft.net/article/46687/download/xml/
Introduction: The aim of this survey is to assess the current knowledge and experience of medical doctors in regard to off-label use and to determine whether it is legal, according to them, as the local current legislation on medicinal products does neither prohibit nor explicitly permit such use.
Materials and methods: One hundred medical doctors from inpatient and outpatient medical care participated and answered 10 questions. The inclusion criteria were to have a degree in medicine and right to prescribe medications. Statistical analysis was performed using the SPSS v19.0 statistical program.
Results: The results show that 8 out of 10 medical doctors have knowledge in regard to the meaning of the term “off-label”. More than half (62%) stated they have prescribed off-label medicine in their medical practice. Half of the respondents (49%) experienced some kind of trouble when prescribing off-label. In regard to whether the law allows this practice, opinions were divided. To a larger extent (88%), medical doctors support the introduction of clearer rules for off-label use in Bulgaria.
Conclusion: Edical doctors have significant knowledge about off-label use, but feel uncertainty in their actions when they use medicines off-label. It is necessary to consider regulating this use at a national level, following the good examples of other European Union (EU) member states, until a harmonized solution within EU is found.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
off-label
pharmacovigilance
survey
The knowledge and experience with the off-label use – results of a survey
Research Article
10.3897/pharmacia.66.e38571
2019-12-31
pharmacia
Medical University of Sofia, Sofia, Bulgaria
author
Yordanov, Yordan
https://orcid.org/0000-0003-1029-9870
2019-12-31
2019-12-31
2019
Pharmacia
2603-557X
0428-0296
66
223-231
2019
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10.3897/pharmacia.66.e38573
10.3897/pharmacia.66.e38571
https://pharmacia.pensoft.net/article/38571/
https://pharmacia.pensoft.net/article/38571/download/pdf/
https://pharmacia.pensoft.net/article/38571/download/xml/
Propolis is a natural product with a plethora of biological effects, utilized by traditional medicine since antiquity. However, its application as a pharmaceutical is hindered by its variable composition and difficult standardization. CAPE has been shown to be a major component of propolis, with a large contribution to its pharmacological effects, among which the anti-inflammatory, antioxidant and antineoplastic have been attracting most attention. The current review article aims to present the cornerstone pharmacological studies of CAPE throughout the years, following its discovery, which confirmed its primary importance among propolis constituents and opened the path to its intensive research as a potential pharmaceutical. We present the diversity of drug delivery systems of CAPE, which have been developed to improve its efficacy in in vitro and in vivo disease models and discuss their primary promises and weaknesses. The increased interest in recent years over more practical approaches of CAPE research such as its pharmaceutical formulation comes to show that it has a potential to become commercialized as a pharmaceutical.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Caffeic acid phenethyl ester
propolis
pharmacological properties
drug delivery systems
Caffeic acid phenethyl ester (CAPE): cornerstone pharmacological studies and drug delivery systems
Review Article
10.3897/pharmacia.66.e37706
2019-12-31
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Zimenkovsky, Andriy
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Nastyukha, Yuliya
https://orcid.org/0000-0001-5281-7168
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Kostyana, Kateryna
https://orcid.org/0000-0003-4688-721X
Uzhhorod National University, Uzhhorod, Ukraine
author
Devinyak, Oleg
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Zayats, Marta
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Koval, Andriy
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Denysiuk, Oksana
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Gorodnycha, Oksana
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Siatynia, Vitaliy
2019-12-31
2019-12-31
2019
Pharmacia
2603-557X
0428-0296
66
193-200
2019
Drug related problems: an over view of various classification systems.
PK
Adusumilli
author
2014
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2014
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10.3897/pharmacia.66.e37706
https://pharmacia.pensoft.net/article/37706/
https://pharmacia.pensoft.net/article/37706/download/pdf/
https://pharmacia.pensoft.net/article/37706/download/xml/
An anonymous questionnaire survey among healthcare professionals in Ukraine (n = 10737) showed contradictions in the attitude to the peculiarities of clinical pharmacy compared to a general pharmacy, the relation to the specialists who provide clinical pharmacy services, and the healthcare settings for them. The respondents considered the activity of clinical pharmacist necessary in 74.2%. The community pharmacies were identified as prior professional settings for clinical pharmacists in 55.3%; hospitals – 47.7%, hospital pharmacies – 45.0%. Among the directions of the clinical pharmacist’s activity at the hospital, monitoring of drug safety and efficacy was stated by 69.9%, provision of the pharmaceutical care – 50.5%, evaluation of pharmacotherapy – 42.1%, processing of the local drug formulary – 26.8%. The respondents saw prospects for the development of clinical pharmacy in Ukraine in 54.2%. Therefore, there were differences in views on the key issues of clinical pharmacy throughout Ukraine and the insufficient support for its prospects.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
clinical pharmacy
clinical pharmacist
healthcare professionals’ opinion
Ukraine
Clinical pharmacy in Ukraine according to the healthcare professionals’ assessment
Research Article
10.3897/pharmacia.66.e36808
2019-12-31
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Drapak, Iryna
https://orcid.org/0000-0002-6830-2908
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Zimenkovsky, Borys
National University of Pharmacy, Kharkiv, Ukraine
author
Perekhoda, Lina
National University of Pharmacy, Kharkiv, Ukraine
author
Suleyman, Мargarita
National University of Pharmacy, Kharkiv, Ukraine
author
Yeromina, Hanna
https://orcid.org/0000-0003-2986-3507
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Skaletska, Natalia
Institute of Pharmacology and Toxicology, National Academy of Medical Sciences, Kyiv, Ukraine
author
Seredynska, Natalya
Institute of Pharmacology and Toxicology, National Academy of Medical Sciences, Kyiv, Ukraine
author
Demchenko, Anatoly
2019-12-31
2019-12-31
2019
Pharmacia
2603-557X
0428-0296
66
181-186
2019
10.1016/j.ejmech.2015.03.065
10.1021/jm200409s
10.1007/s10593-005-0200-x
10.3390/scipharm87010007
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Drapak
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Perekhoda
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Yeromina
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2016
text
Der Pharma Chemica
2016
8
3
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10.3897/pharmacia.66.e36808
https://pharmacia.pensoft.net/article/36808/
https://pharmacia.pensoft.net/article/36808/download/pdf/
https://pharmacia.pensoft.net/article/36808/download/xml/
The aim of study was to find potential antihypertensive and cardiotropic drugs among new 4-aryl-N-(aryl)-3-(prop-2-en-1-yl)-2,3-dihydro-1,3-thiazol-2-imines.
Materials and methods: The target compounds were synthesized by condensation asymmetrical substituted thioureas with α-bromo-4-R1-acetophenones in ethanol medium. The structure and purity of the compounds synthesized were confirmed by 1H, 13C NMR-spectroscopy and elemental analysis. Docking studies of synthesized compounds to the active site of angiotensin receptor ІІ (PDB ID: 3R8A) were performed in order to find its potential inhibitors and to select promising compounds for experimental screening. Pharmacological studies of the influence on the cardiovascular system were performed.
Results: The results of docking studies indicate a high affinity of all tested substances to the selected biotarget. The thermodynamic probability of binding of synthesized substances to protein 3R8A was confirmed by negative values of scoring functions. Hydrobromide of 4-(4-methoxyphenyl)-N-phenyl-3-(prop-2-en-1-yl)-2,3-dihydro-1,3-thiazol-2-imine 3(1) and hydrobrmide of 4-(4-methoxyphenyl)-N-(4-bromphenyl)-3-(prop-2-en-1-yl)-2,3-dihydro-1,3-thiazol-2-imine 3(3), which have the highest negative values of scoring functions, are recommended for in vivo pharmacological studies. Based on a complete analysis of the geometric location of the synthesized compounds (ligands) in the active site of the angiotensin II receptor, it was found that the complexes are formed with the involvement of Nitrogen atom of imino group, the 1,3-thiazole ring, the phenyl and alkyl moieties of the molecule form hydrogen bonds, intermolecular electrostatic and donor-acceptor interactions. The conducted pharmacological studies of the influence on the cardiovascular system have allowed to confirm the presence of antihypertensive effect inherent in compounds of this series (except for compound 3(2)). The most effective antihypertensive effect, which is similar in duration and strength of the effect of valsartan, was the effect of compound 3(5).
Conclusions: In order to expand the arsenal of biologically active substances of cardiotropic action a systematic series of new 4-aryl-N-(aryl)-3-(prop-2-en-1-yl)-2,3-dihydro-1,3-thiazol-2-imine derivatives were synthesized. The structure and purity of the compounds synthesized were confirmed by 1H, 13C NMR-spectroscopy and elemental analysis. Based on the results of docking studies using Autodock 4.2.6 software, selected compounds with the best affinity for protein biomes (PDB codes: 3R8A) are promising for experimental studies of hypotensive and cardiotropic activity. The most effective antihypertensive effect, which is similar in duration and strength of the effect of valsartan, was the effect of compound 3(5). A comparative analysis of the results of molecular docking and in vivo results suggests that there is a positive correlation between scoring protein inhibition and experimental data.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
synthesis
4-aryl-N-(aryl)-3-(prop-2-en-1-yl)-2
3-dihydro-1
3-thiazol-2-imine derivatives
molecular docking
angiotensin receptor ІІ antagonists
Search for angiotensin II receptor antagonists among 4-aryl-n-(aryl)-3-(prop-2-en-1-yl)-2,3-dihydro-1,3-thiazol-2-imine derivatives
Research Article
10.3897/pharmacia.66.e36764
2019-12-31
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Chaban, Taras
https://orcid.org/0000-0003-0618-275X
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Ogurtsov, Volodymyr
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Mahlovanyy, Anatoliy
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Sukhodolska, Natalia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Chaban, Ihor
Burgas University “Prof. Dr. Asen Zlatarov”, Burgas, Bulgaria
author
Harkov, Stefan
https://orcid.org/0000-0003-1012-849X
Ivan Franko National University of Lviv, Lviv, Ukraine
author
Matiychuk, Vasyl
2019-12-31
2019-12-31
2019
Pharmacia
2603-557X
0428-0296
66
171-180
2019
10.1016/j.foodchem.2010.01.009
10.1038/1811199a0
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Synthesis of novel thiazolo[4,5-b]pyridines as potential biologically active substances.
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Chaban
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534
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Chaban
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Chaban
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Pharmacia
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2018a
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10.17344/acsi.2018.4570
10.3897/pharmacia.66.e35131
10.23939/chcht07.04.397
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Klenina
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Molyneux
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10.3897/pharmacia.66.e36764
https://pharmacia.pensoft.net/article/36764/
https://pharmacia.pensoft.net/article/36764/download/pdf/
https://pharmacia.pensoft.net/article/36764/download/xml/
The synthesis and determination of the antioxidant activity of some novel (5,7-dimethyl-2-oxo-thiazolo[4,5-b]pyridine-3-yl)-acetic acid hydrazide derivatives are described in this article. The transformation of the base heterocycle was carried out via the reactions of acylation, [2+3]cyclocondensation, Knoevenagel condencation and alkylation for the purpose to obtain substances with a satisfactory pharmacological profile. Antioxidant activity of the synthesized compounds was evaluated in vitro by means of the scavenging metod effect on 2,2 diphenyl-1-picrylhydrazyl (DPPH) radicals.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
antioxidant activity
2
2 diphenyl-1-picrylhydrazyl
synthesis
thiazolo[4
5-b]pyridines
Antioxidant properties of some novel derivatives thiazolo[4,5-b] pyridine
Research Article
10.3897/pharmacia.66.e38976
2019-12-31
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Hudz, Nataliia
https://orcid.org/0000-0002-2240-0852
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Yezerska, Oksana
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Shanaida, Mariia
Institute of Biodiversity Conservation and Biosafety, Slovak University of Agriculture in Nitra, Nitra, Slovakia
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Pervaiz, Roomna
https://orcid.org/0000-0002-4522-1988
University of Opole, Opole, Poland
author
Wieczorek, Piotr P.
2019-12-31
2019-12-31
2019
Pharmacia
2603-557X
0428-0296
66
209-215
2019
Phytochemical study and biological activity of sage (Salvia officinalis L.).
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10.15835/nsb325638
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10.3897/pharmacia.66.e38976
https://pharmacia.pensoft.net/article/38976/
https://pharmacia.pensoft.net/article/38976/download/pdf/
https://pharmacia.pensoft.net/article/38976/download/xml/
Polyphenols are valuable group of phytoconstituents due to their high antioxidant activity and healing properties. Antioxidant properties of sages are attributed mainly to a high level of phenolic compounds. The aim of the present study was to elaborate an analytical procedure for the evaluation of the content of secondary metabolites of the polyphenol nature in the herb of Clary Sage (Salvia sclarea L.). Four crude extracts of Salvia sclarea herb obtained with different technologies were used to develop an analytical procedure for the total phenolic content (TPC) assay by spectrophotometric method. The optimum conditions for the analysis (time of the reaction, wavelength, and reference substances) were chosen and experimentally justified (60–80 min, 760 nm, gallic acid and rutin, respectively). Under these conditions, the developed analytical procedure is robust in the indicated time and easy for performing in phytochemical or technological laboratories. The yield of TPC from the herb of Salvia sclarea was the highest in the extracts prepared by heating at a temperature of 36–46 °C and with using the ultrasonic bath. TPC was the highest in the extract in which solvent-to-herb ratio was the least (10:1) and particle size was in the range of 2–5 mm. As a result of the studies, the analytical procedure of the determination of TPC was developed and its parameters were justified. This methodology complies with the requirements for pharmaceutical analysis to ensure the reliability of results during pharmaceutical development and routine control of Salvia sclarea extracts.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Salvia sclarea
herb
extract
Folin-Ciocalteu reagent
total phenolic content
Application of the Folin-Ciocalteu method to the evaluation of Salvia sclarea extracts
Research Article
10.3897/pharmacia.66.e37893
2019-12-31
pharmacia
National University of Pharmacy, Kharkiv, Ukraine
author
Iosypenko, Olena
https://orcid.org/0000-0003-1613-2828
National University of Pharmacy, Kharkiv, Ukraine
author
Zhuravel, Iryna
National University of Pharmacy, Kharkiv, Ukraine
author
Omelchenko, Zinaida
National University of Pharmacy, Kharkiv, Ukraine
author
Burlaka, Iryna
2019-12-31
2019-12-31
2019
Pharmacia
2603-557X
0428-0296
66
201-207
2019
10.1201/9781439823385
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Batyuchenko
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Ukrainian Medical Almanac
2013
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Fats and fatty acids in human nutrition – Report of an expert consultation.
2010
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10.14739/2409-2932.2019.2.170978
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Levachev
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Levitsky
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Omelchenko
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Plemenkov
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Sakhno
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Semenov
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Van Wyk
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10.3897/pharmacia.66.e37893
https://pharmacia.pensoft.net/article/37893/
https://pharmacia.pensoft.net/article/37893/download/pdf/
https://pharmacia.pensoft.net/article/37893/download/xml/
The qualitative composition and quantitative content of fatty acids in leaves of vegetable marrows (Cucurbita pepo L. var. giromontina Alef.), zucchini (Cucurbita pepo L. var. cylindrica Paris) yellow- and green-fruits varieties were determined by using GC/MS. 14 fatty acids were identified as the result of the experiment. Unsaturated fatty acids were found to be dominated in the raw plant material. The total content of unsaturated fatty acids amounted to 60.47% in vegetable marrows leaves, 64.35% yellow zucchini leaves and 68.85% green zucchini leaves, among which linoleic and linolenic acid dominated. The related health lipid indices (IA, atherogenicity, IT, thrombogenicity and IH, health) were determined. It is shown that the use of such biological resources is actual and expedient for the purpose of alimentary correction of the physiological condition of a person.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
vegetable marrows
zucchini
leaves
GC/MS
fatty acids
indices: atherogenicity
thrombogenicity and health
Fatty acid composition of vegetable marrows and zucchini leaves
Research Article
10.3897/pharmacia.66.e35194
2019-12-31
pharmacia
National University of Pharmacy, Kharkiv, Ukraine
author
Kyslychenko, Aleksandra
National University of Pharmacy, Kharkiv, Ukraine
author
Процкая, Viktoriia
https://orcid.org/0000-0002-2439-138X
National University of Pharmacy, Kharkiv, Ukraine
author
Zhuravel, Iryna
2019-12-31
2019-12-31
2019
Pharmacia
2603-557X
0428-0296
66
161-164
2019
10.18203/2319-2003.ijbcp20175072
10.3109/13880209.2012.654396
Lichen Secondary Metabolites and Its Biological Activity.
S
Bhattacharyya
author
2016
text
American Journal of PharmTech Research
2016
6
6
28
44
The Antimicrobial Properties of Extracts Isolated from Lichen Parmelia vagans.
V
Bondarenko
author
2017
text
FASEB journal
2017
31
1
1
10
10.1079/PGR200572
10.1007/s00114-002-0305-3
10.1017/CBO9780511790478.008
10.1039/C4RA09104C
10.7897/2277-4343.07138
Development and validation of a RP-HPLC method for the simultaneous determination of luteolin and apigenin in herb of Achillea millefolium L.
A
Gudzenko
author
2013
text
The pharma innovation – journal
2013
2
7
7
14
10.11603/mcch.2410-681X.2018.v0.i1.8754
The study of volatile compounds of parmelia perlata thallus.
OA
Kyslychenko
author
2018b
text
Pharmacia
2018b
65
3
11
16
10.1017/S0024282911000521
Phytochemical constituents from different species of parmelia genus: A review.
AK
Sharma
author
2013
text
Der Chemica Sinica
2013
4
1
1
11
Biologically Active Substances of Lichens.
AI
Shcherbakova
author
2013
text
Forest Journal
2013
3
7
16
10.2174/1389557517666170425105727
10.3897/pharmacia.66.e35194
https://pharmacia.pensoft.net/article/35194/
https://pharmacia.pensoft.net/article/35194/download/pdf/
https://pharmacia.pensoft.net/article/35194/download/xml/
The species of Parmelia genus have long been used in Indian folk medicine for the treatment of bronchitis, ulcers, furunculosis, cardiovascular diseases, urolithiasis, amenorrhea, and also at infectious and inflammatory diseases. In Ukraine, the most common lichens of the Parmelia genus are Parmelia sulcata Tailor and Parmelia vagans Nyl. At the same time, thalli of Parmelia genus lichens belong to the non-officinal and poorly studied types of raw material.
The qualitative composition and the quantitative content of phenolic compounds in Parmelia sulcata and Parmelia vagans thalli was studied by HPLC.
According to the results of the chromatographic analysis, salazinic, fumaroprotocetraric, usnic acids, chloratranorin and atranorin were identified in both types of raw material studied. In addition, protocetraric acid was identified in Parmelia sulcata thalli.
According to the results of the experiment, the total content of identified phenolic compounds in Parmelia sulcata thalli was 2019.71±40.39 g/mol, and in Parmelia vagans thalli it comprised 1754.18±34.77 g/mol.
In the thalli of both studied species of Parmelia genus, fumaroprotocetraric acid dominanted by the quantity. This substance was present in Parmelia sulcata thalli in the amount of 474.00±9.00 g/mol, and in Parmelia vagans thalli – 456.21±8.67 g/mol.
In addition, a significant amount of chloratranorin (408.79±8.99 g/mol) was present in Parmelia sulcata thalli. Quite a high content of atranorin (393.34±8.65 g/mol) and usnic acid (375.31±7.53 g/mol) were defined in Parmelia vagans thalli.
The results obtained can be used in the development of quality control methods for Parmelia sulcata and Parmelia vagans thalli, as well as medicines based on these types of raw materials.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
HPLC
lichen
lichen acids
Parmelia sulcata
Parmelia vagans
phenolic compounds
HPLC determination of phenolic compounds content in Parmelia sulcata and Parmelia vagans thalli
Research Article
10.3897/pharmacia.66.e37267
2019-12-31
pharmacia
National University of Pharmacy, Kharkiv, Ukraine
author
Postoy, Vladyslav
National University of Pharmacy, Kharkiv, Ukraine
author
Kukhtenko, Halina Kukhtenko
National University of Pharmacy, Kharkiv, Ukraine
author
Vyshnevska, Liliia
https://orcid.org/0000-0002-6887-3591
National University of Pharmacy, Kharkiv, Ukraine
author
Gladukh, Ievgenii
https://orcid.org/0000-0002-5739-9257
National University of Pharmacy, Kharkiv, Ukraine
author
Semchenko, Kateryna
https://orcid.org/0000-0003-3824-8899
2019-12-31
2019-12-31
2019
Pharmacia
2603-557X
0428-0296
66
187-192
2019
10.24959/nphj.15.2076
Influence of Excipients on the Structural and Mechanical Properties of Semisolid Dosage Forms.
H
Kukhtenko
author
2017
text
Asian Journal of Pharmaceutics
2017
11
3
575
578
Place of the dolobene gel preparation in the treatment of patients with rheumatic diseases.Ukrainian Rheumatologic Journal.
NM
Shuba
author
2007
text
LLC “MORION”
2007
2
28
56
60
The effect of the PEG-40 solubilizer hydrogenated castor oil on the structural and mechanical properties of carbopol gel.
EV
Gladukh
author
2017
text
Actual Pharmaceutical and Medical Science Practices
2017
3
25
288
295
10.14739/2409-2932.2017.3.113552
VN
Kovalenko
author
2003
2003
GP
Kuhtenko
author
2017
2017
VA
Nasonova
author
2001
2001
10.3897/pharmacia.66.e37267
https://pharmacia.pensoft.net/article/37267/
https://pharmacia.pensoft.net/article/37267/download/pdf/
https://pharmacia.pensoft.net/article/37267/download/xml/
The aim of the research was to investigate the rheological behaviour of hydroxyethyl cellulose gels, which are used in the development of the composition and technology of the medicine with anti-inflammatory activity. The objects of research were hydroxyethyl cellulose gels in the concentration of 1.0%; 1.5%; 2.0%; 2.5% and 3.0%. Investigation of the rheological behavior of HEC gels (structural and mechanical properties) was performed using the Rheolab QC rheometer from Anton Paar (Austria) using the C-CC27 / SS Coaxial Cylinder System. The obtained results showed that excipients influence on the rheological behaviour in the following way: propylene glycol and polyethylene oxide 400 reduce rheoparameters, while PEG 40 hydrogenated castor oil increases these parameters. The further study of the rheological properties in comparison with medicines “Troxerutin” and “Thiotriazolinum” showed that the developed gel fully corresponds to technological and consumer requirements.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
rheological paramets
gel
hydroxyethyl cellulose
Study of rheological behaviour of hydroxyethyl cellulose gels in the development of the composition and technology of the medicine with anti-inflammatory activity
Research Article
10.3897/pharmacia.66.e37997
2019-12-31
pharmacia
Medical University of Sofia, Sofia, Bulgaria
author
Kondeva-Burdina, Magdalena
Medical University of Sofia, Sofia, Bulgaria
author
Krasteva, Ilina
University of Forestry, Sofia, Bulgaria
author
Popov, Georgi
University of Forestry, Sofia, Bulgaria
author
Krasteva, Ilina
https://orcid.org/0000-0001-8559-0369
2019-12-31
2019-12-31
2019
Pharmacia
2603-557X
0428-0296
66
233-236
2019
10.4103/0973-1296.139778
Protein measurement with the Folin phenol reagent.
OH
Lowry
author
1951
text
Journal of Biological Chemistry
1951
193
1
265
275
10.1016/j.neulet.2004.03.036
10.1080/13880200802367247
Protective effect of purified saponin mixture from Astragalus corniculatus on toxicity models in vitro.
M
Mitcheva
author
S
Tonev
author
2009
text
Publishing House IRITA, Sofia
2009
239
251
10.1002/jnr.10193
Effect of а purified saponins’ mixture from Astragalus glycyphylloides, administered alone, on isolated rat brain synaptosomes and hepatocytes.
G
Popov
author
2018
text
Tradition and Modernity in Veterinary Medicine
2018
2
5
43
49
10.1016/0003-9861(71)90334-1
10.1016/j.phytol.2018.05.015
10.1046/j.1471-4159.1994.62041586.x
10.1016/j.jep.2011.12.023
10.3897/pharmacia.66.e37997
https://pharmacia.pensoft.net/article/37997/
https://pharmacia.pensoft.net/article/37997/download/pdf/
https://pharmacia.pensoft.net/article/37997/download/xml/
The aim of the study was to investigate the possible neuroprotective and antioxidant activity of purified saponins’mixture (PSM), isolated from Astragalus glycyphylloides (Fabaceae), in a model of 6-hydroxydipamine (6-OHDA)-induced oxidative stress on isolated rat brain synaptosomes. Synaptosomes were incubated with 3 different concentrations of PSM: 60 µg/mL; 6 µg/mL; 0.6 µg/mL. The effects of PSM were compared to those of silymarin (S), at the same concentrations. The main parameters, characterized functional and metabolic status of synaptosomes, were investigated: viability (MTT-test) and level of reduced glutathione (GSH). At isolated rat brain synaptosomes, in conditions of 6-OHDA-induced oxidative stress (150 μМ), PSM revealed statistically significant, concentration-dependent, neuroprotective and antioxidant effects, compared to those of silymarin. Effects were most prominent at concentration 60 µg/mL. These neuroprotective effects of PSM might be due to the possible activity as scavenger of reactive oxygen species (ROS), produced by p-quinone (toxic metabolite of 6-OHDA).
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Astragalus glycyphylloides
saponins’ mixture
synaptosomes
neuroprotection
antioxidant
Neuroprotective and antioxidant activities of saponins’ mixture from Astragalus glycyphylloides in a model of 6-hydroxydopamine-induced oxidative stress on isolated rat brain synaptosomes
Research Article
10.3897/pharmacia.67.e37004
2020-05-13
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Drapak, Iryna
https://orcid.org/0000-0002-6830-2908
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Zimenkovsky, Borys
Lithuanian University of Health Sciences, Kaunas, Lithuania
author
Ivanauskas, Liudas
National University of Pharmacy, Kharkiv, Ukraine
author
Bezruk, Ivan
https://orcid.org/0000-0002-1212-1649
National University of Pharmacy, Kharkiv, Ukraine
author
Perekhoda, Lina
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Muzychenko, Volodymir
I. Horbachevsky Ternopil State Medical University, Ternopil, Ukraine
author
Logoyda, Liliya
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Demchuk, Inna
2020-05-13
2020-05-13
2020
Pharmacia
2603-557X
0428-0296
67
1
29-37
2020
10.3897/pharmacia.67.e37004
https://pharmacia.pensoft.net/article/37004/
https://pharmacia.pensoft.net/article/37004/download/pdf/
https://pharmacia.pensoft.net/article/37004/download/xml/
Aim. The aim of study was to develop a simple and accurate procedure that could be applied for the determination of impurities and degradation products in cardiazol. Materials and methods. Separation in samples was carried out with Acquity H-class UPLC system (Waters, Milford, USA) equipped with Acquity UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm) (Waters, Milford, USA). Xevo TQD triple quadrupole mass spectrometer detector (Waters Millford, USA) was used to obtain MS/MS data. Mobile phase A: 0.1% solution of trifluoroacetic acid R in water R; Mobile phase B: acetonitrile R. Samples were chromatographed in gradient mode (Table 1). Flow rate of the mobile phase: 1 ml / min. Column temperature: 30 °С. Detection: at 240 nm wavelength. Injection volume: 10 μl. Results. The retention time of the main substance is about 18.5 minutes. The order of the peak, the retention times and relative retention times: impurity B (12.04, 0.65); impurity А (18.5; 0.98); Cardiazol (18.87; 1.00). The LOD and LOQ values obtained were in the range of 30 ng/mL to 100 ng/mL and 80 ng/mL to 310 ng/mL respectively (with respect to sample concentration of 2 mg/ml). Linearity was established in the range of LOQ level to 0.2% having regression coefficients in the range of 0.9996 to 0.9999. The change in the temperature of the column affects the degree of separation of cardiazol and the impurity A, and thus, with a decrease of 5 ° C, the degree of separation is (1.06), while with increasing this index (3.43). When changing the flow rate of the mobile phase, the degree of separation changes in the following order, with a decrease to 0.9 ml / min separation (1.90), with an increase in speed to 1.1 ml / min (2.45). When the number of mobile phase B decreases by 5%, the degree of separation varies by (2.65), with an increase of 5% (1.82). In comparison with the chromatogram of the tested solution, the substance is not resistant to the action of peroxide, alkaline and acid decomposition. Conclusion. 1) HPLC method was developed and validated for the simultaneous detection and quantitation of impurities formed during the synthesis of cardiazol. 2) The method proved to be sensitive, selective, precise, linear, accurate and stability-indicating.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
HPLC
Cardiazol
Impurities
Degradation Products
HPLC method for simultaneous determination of impurities and degradation products in Cardiazol
Research Article
10.3897/pharmacia.67.e36150
2020-05-13
pharmacia
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Popovych, Zoryana
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Rozhko, Mykola
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Ostapyak, Iryna
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Oktysyuk, Yuriy
https://orcid.org/0000-0001-7741-2490
2020-05-13
2020-05-13
2020
Pharmacia
2603-557X
0428-0296
67
1
23-28
2020
10.3897/pharmacia.67.e36150
https://pharmacia.pensoft.net/article/36150/
https://pharmacia.pensoft.net/article/36150/download/pdf/
https://pharmacia.pensoft.net/article/36150/download/xml/
To be living at the most advanced technological area in history is to exist and face pollution one of the most serious threats to all living things on the planet. It is a well known fact that industrial pollutions cause a considerable number of health problems and stomatological in particular. This article demonstrates the results of our study regarding investigation ofsome biochemical indexes in children and adults from the polluted area. We have examined 1236 scholars and 133 workers of Burstyn Thermoelectric Station. The findings suggest that the prevalence of stomatological disorders reaches up to 84,2–93,5% in all examined, the statistic shows also that 31,6 % of six year old children have decayed teeth, in 12 year old children this index is more than 76,8% and almost 92,3% in those aged15–17years. Symptoms of periodontal disorders were observed in 37,1–78,3% of children, the orthodontic pathology was diagnosed in 43,9–61,2% of all examined scholars. People that work at the Burstyn Thermoelectric Station are affected by periodontal pathology and demonstrated disordered mineral metabolism in the blood serum and oral liquid with decreased level of Calcium and increased amount of Phosphates.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
biochemical indexes
chronic periodontitis
dental caries
blood serum
oral liquid
Biochemical indexes of mineral metabolismin patients from the polluted regionaffected by chronic periodontitis
Research Article
10.3897/pharmacia.67.e35152
2020-05-13
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Olha Ripetska, Olha
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Mahlovanyy, Anatoliy
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Hrynovets, Ihor
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Chaban, Taras
https://orcid.org/0000-0003-0618-275X
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Hrynovets, Volodymyr
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Buchkovska, Anna
Medical College of Burgas University “Prof. Dr. Asen Zlatarov”, Burgas, Bulgaria
author
Harkov, Stefan
https://orcid.org/0000-0003-1012-849X
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Deneha, Ihor
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Pasko, Oksana
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Renka, Marta
2020-05-13
2020-05-13
2020
Pharmacia
2603-557X
0428-0296
67
1
17-22
2020
10.3897/pharmacia.67.e35152
https://pharmacia.pensoft.net/article/35152/
https://pharmacia.pensoft.net/article/35152/download/pdf/
https://pharmacia.pensoft.net/article/35152/download/xml/
Silicon-containing (Si-containing) polishing paste has been tested clinically in patients with chronic generalized periodontitis, I-st degree of severity. All patients have been examined for the presence of supra- and sub-gingival calculus and bleeding on probing on a six- month-testing time and within one year after conducting the professional hygienic procedures. It has been revealed that the proposed Si-containing polishing paste proved to be effective in the maintenance of gingival health in the lapse of time from 1 to 12 months after treatment. The majority of patients showed neither dental deposits nor bleeding on probing up in the interval from 1 to 12 months after dental scaling and polishing with Si-containing polishing paste.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
bleeding
dental calculus
dental plaque
dental scaling
generalized periodontitis
Si-containing polishing paste
Clinical study of the efficacy of si-containing polishing paste for the professional hygiene procedures in patients with periodontal disease
Research Article
10.3897/pharmacia.67.e35147
2020-05-13
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Furdychko, Anastasiya
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Buchkovska, Anna
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Petryshyn, Olga
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Hrynovets, Volodymyr
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Chaban, Taras
https://orcid.org/0000-0003-0618-275X
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Hrynovets, Ihor
Medical College of Burgas University “Prof. Dr. Asen Zlatarov”, Burgas, Bulgaria
author
Harkov, Stefan
https://orcid.org/0000-0003-1012-849X
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Chaban, Ihor
2020-05-13
2020-05-13
2020
Pharmacia
2603-557X
0428-0296
67
1
13-16
2020
10.3897/pharmacia.67.e35147
https://pharmacia.pensoft.net/article/35147/
https://pharmacia.pensoft.net/article/35147/download/pdf/
https://pharmacia.pensoft.net/article/35147/download/xml/
The pathology of the hepatobiliary system significantly affects the state of the organs of the oral cavity. A clinical and index study of the state of periodontal tissues in 122 patients was conducted, which showed the prevalence of periodontal disease in patients with chronic acalculous cholecystitis. The examined patients were divided into groups depending on the treatment method, and the result was evaluated before treatment, directly after treatment and 6 months following treatment. Antidysbiotic hepatoprotector and 2% thiotriazolin ointment were included in the combination therapy. Studies have shown that patients with hepatobiliary pathology have increased likelihood of periodontal diseases, and after treatment, periodontal indicators and the hygiene index are significantly reduced, especially in the group where the proposed scheme was used, and the long-term results were stable for 6 months.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
hepatobiliary pathology
hepatoprotector
prebiotic
periodontitis
thiotriazolin
Combination of general and local action drugs in treatment of patients with generalized periodontitis on the basis of the pathology of the hepatobilary system
Research Article
10.3897/pharmacia.67.e35126
2020-05-13
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Dovhanyk, Viktoria
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Maglyovanyi, Anatoliy
Medical College of Burgas University “Prof. Dr. Asen Zlatarov”, Burgas, Bulgaria
author
Harkov, Stefan
https://orcid.org/0000-0003-1012-849X
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Synytsa, Volodymyr
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Hrynovets, Volodymyr
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Hrynovets, Ihor
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Chaban, Ihor
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Lelyukh, Maryan
https://orcid.org/0000-0002-8123-0988
2020-05-13
2020-05-13
2020
Pharmacia
2603-557X
0428-0296
67
1
5-12
2020
10.3897/pharmacia.67.e35126
https://pharmacia.pensoft.net/article/35126/
https://pharmacia.pensoft.net/article/35126/download/pdf/
https://pharmacia.pensoft.net/article/35126/download/xml/
At the present stage of development of medical science, experimental and clinical dentistry is often used in scientific research, which allows diagnosing preclinical stages of manifestations of changes in tissues and organs of the oral cavity and biological fluids at the level of nanotechnology enabling to study the mechanisms of molecular transformation that lead to occurrence of pathological changes. One of the promising methods of research is infrared spectroscopy. The advantage of the method is that the absorption spectrum can be obtained with a small amount of the test substance (0.1–0.01 cm3) and under conditions of different aggregate states (solution, powder, liquid). Graphical abstract
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
infrared spectroscopy
hydroxyapatite
carbamide peroxide
Spectroscopic criteria for early diagnosis of changes in the mineral and organic matrix of hard dental tissues
Research Article
10.3897/pharmacia.67.e35117
2020-05-13
pharmacia
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Yarmoshuk, Iryna
2020-05-13
2020-05-13
2020
Pharmacia
2603-557X
0428-0296
67
1
1-4
2020
10.3897/pharmacia.67.e35117
https://pharmacia.pensoft.net/article/35117/
https://pharmacia.pensoft.net/article/35117/download/pdf/
https://pharmacia.pensoft.net/article/35117/download/xml/
One of the main mechanisms in pathogenesis of chronic periodontitis is disordered bone metabolism that is accomplished by cortical plate’s resorption, destruction of the interdental septums and osteoporosis of the jaws. Administration of osteotropic agents will allow achieving efficient results in treatment of the patients affected by periodontal disorders, lower the tendency of bone resorption and stimulate the processes of regeneration. The article demonstrates the results obtained after the surgical treatment of the patients with osteopenia affected by chronic periodontitis of II–III degree that were administered simultaneously with synthetic osteoplastic material Tricalcium phosphate and a bone resorption inhibitor Ibandronic acid. It has been established the high efficiency of the recommended treatment that was proved by positive progress of biochemical markers of bone metabolism in blood serum and urine of the individuals from the main group taken 24 month after the beginning of the treatment. The study shows that the reis arise of osteocalcin by 11,5% in the blood serum of the main group patients 2 years after the research and the amount of desoxypiridynolin, on the other hand, was by 21,72% lower comparing with those from the main group (р < 0,05).
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
bisphosphonates
bone tissue
chronic periodontitis
synthetic osteoplastic material
Correction of disordered mineral bone metabolism in patients affected by chronic periodontitis
Research Article
10.3897/pharmacia.67.e50397
2020-07-31
pharmacia
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Horyn, Mariana
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Logoyda, Liliya
2020-07-31
2020-07-31
2020
Pharmacia
2603-557X
0428-0296
67
39-48
2020
10.3897/pharmacia.67.e50397
https://pharmacia.pensoft.net/article/50397/
https://pharmacia.pensoft.net/article/50397/download/pdf/
https://pharmacia.pensoft.net/article/50397/download/xml/
Aim.The main purpose of this study was to develop a simple, precise, rapid and accurate method for the quantification of metoprolol and meldonium in human plasma. Materials and methods. The resolution of peaks of metoprolol was best achieved with Discovery C18, 50 × 2.1 mm, 5 μm column and meldonium - ZORBAX HILIC Plus, 50 × 2.1 mm, 3.5 μm column. Samples of metoprolol were chromatographed in a gradient mode (eluent A (acetonitrile – water – formic acid, 5 : 95 : 0.1 v/v), eluent B (acetonitrile – formic acid, 100 : 0.1 v/v)). The initial content of the eluent B is 0%, which increases linearly by 1.0 min to 100% and to 1.11 min returns to the initial 0%. The mobile phase was delivered at a flow rate of 0.400 mL/min into the mass spectrometer ESI chamber. The injection volume was 5μl. Samples of meldonium were chromatographed in a isocratic using mobile phase water – acetonitrile – ammonium formate buffer 200 мМ, 20 : 75 : 5 v/v). Results.The total chromatographic run time was 2.0 minutes and the elution of metoprolol, meldonium and IS occurred at ~1.39 and 1.18 minutes, respectively.A linear response function was established at 2 - 200 ng/mL for metoprolol and 50 -5000 ng/mL for meldonium in human plasma. The% mean recovery for metoprolol in LQC, MQC and HQC was 99.0%, 107.5% and 96.8%, for meldonium in LQC, MQC and HQC was 94.1%, 100.2% and 93.1% respectively. The lowest concentration with the RSD <20% was taken as LLOQ and was found to be 2.31 ng/mL for metoprolol, 47.70 ng/mL for meldonium. The % accuracy of LLOQ samples prepared with the different biological matrix lots were found 115.4% for metoprolol and 95.5% for meldonium, which were found within the range of 80.00–120.00% for the seven different plasma lots. % CV for LLOQ samples was observed as 12.8% and 7.7% respectively, which are within 20.00% of the acceptance criteria. Conclusion.A rapid method was developed for simultaneous determination of metoprolol and meldonium in human plasma. The method was strictly validated according to the ICH guidelines. Acquired results demonstrate that proposed strategy can be effortlessly and advantageously applied for routine examination of metoprolol and meldonium in human plasma.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
LC-MS/MS
Metoprolol
Meldonium
Validation
Human plasma
Bioanalytical method development and validation for the determination of metoprolol and meldonium in human plasma
Research Article
10.3897/pharmacia.67.e38648
2020-07-31
pharmacia
Medical University of Sofia, Sofia, Bulgaria
author
Yoncheva, Krassimira
https://orcid.org/0000-0003-2704-3054
Medical University of Sofia, Sofia, Bulgaria
author
Hristova-Avakumova, Nadia
Medical University of Sofia, Sofia, Bulgaria
author
Hadjimitova, Vera
Medical University of Sofia, Sofia, Bulgaria
author
Traykov, Trayko
Institute of Polymers, Bulgarian Academy of Sciences, Sofia, Bulgaria
author
Petrov, Petar
2020-07-31
2020-07-31
2020
Pharmacia
2603-557X
0428-0296
67
49-54
2020
10.3897/pharmacia.67.e38648
https://pharmacia.pensoft.net/article/38648/
https://pharmacia.pensoft.net/article/38648/download/pdf/
https://pharmacia.pensoft.net/article/38648/download/xml/
The study was focused on the evaluation of two copolymers as micellar carriers for kaempferol delivery. The copolymers comprised identical hydrophilic blocks of poly(2-(dimethylamino)ethyl methacrylate and different hydrophobic blocks of either poly(ε-caprolactone) (PDMAEMA9-b-PCL70-b-PDMAEMA9) or poly(propylene oxide) (PDMAEMA13-b-PPO69-b-PDMAEMA13). The calculation of Flory-Huggins parameters and determination of encapsulation efficiency showed that PDMAEMA-b-PCL-b-PDMAEMA copolymer possessed higher capacity for kaempferol loading. The diameter of the micelles before and after lyophilization was not changed, suggesting that the micelles could be lyophilized and redispersed before administration. The in vitro release of kaempferol from PDMAEMA-b-PPO-b-PDMAEMA micelles was faster than the release from PDMAEMA-b-PCL-b-PDMAEMA micelles, probably due to the higher affinity of kaempferol to this copolymer. Further, the higher affinity resulted in a retention of antioxidant activity of kaempferol in the presence of DPPH and KO2 radicals. Thus, PDMAEMA-PCL-PDMAEMA was considered more appropriate carrier because of the higher encapsulation efficiency and preservation of antioxidant activity of the drug.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
copolymeric micelles
nanosized systems
kaempferol
radical scavenging activity
Evaluation of physicochemical and antioxidant properties of nanosized copolymeric micelles loaded with kaempferol
Research Article
10.3897/pharmacia.67.e48220
2020-07-31
pharmacia
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Logoyda, Liliya
2020-07-31
2020-07-31
2020
Pharmacia
2603-557X
0428-0296
67
55-61
2020
10.3897/pharmacia.67.e48220
https://pharmacia.pensoft.net/article/48220/
https://pharmacia.pensoft.net/article/48220/download/pdf/
https://pharmacia.pensoft.net/article/48220/download/xml/
Aim. A rapid and reproducible HPLC method has been developed for the determination of amlodipine in experimental combined dosage forms containing amlodipine, bisoprolol and enalapril and for drug dissolution studies. Materials and methods. The separation was done using a column Phenomenex Polar Synergi, 5 μm, 4.6×50 mm and a mobile phase of methanol:phosphate buffer solution (65:35, v/v), flow-rate of 1.0 mL/min. The injection volume was 100 μL and the ultraviolet detector was set at 240 nm. Results. The method was validated as per ICH guidelines. Under these conditions, amlodipine was eluted at 1.89 min. Total run time was shorter than 2.5 min. The linearity of the method had a good correlation with concentration and peak area. The correlation coefficient of amlodipine was found to be not less than 0.9991, which indicates good linear relationship over concentration range 0.625 mg/mL–5.000 mg/mL (1.250 mg/mL–5.000 mg/mL at pH 4.5). The % RSD values in intra-day and inter-day precision study were found to be less than 0.267 for amlodipine, which indicate method was precise. Hence, the present developed method was said to be suitable for the analysis of drugs in their pharmaceutical dosage form. Also, in vitro dissolution of amlodipine containing tablets were performed to validate the suitability of the proposed method. The dissolution pattern complies with the FDA standards, indicating suitability of the proposed method for the dissolution study of amlodipine. It will allow conducting comparative studies in vitro to confirm the equivalence of tablets containing amlodipine. Conclusion. A simple and sensetive HPLC method was developed for the estimation of amlodipine in tablets containing amlodipine, enalapril and bisoprolol. The proposed method was applied successfully for quality control assay of amlodipine in experimental tablets and in vitro dissolution studies. In vitro / in vivo correlation of amlodipine has been conducted.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Amlodipine
High performance liquid chromatography
Dissolution study
Validation
In vitro / in vivo correlation
Efficient validated method of HPLC to determine amlodipine in combinated dosage form containing amlodipine, enalapril and bisoprolol and in vitro dissolution studies with in vitro/ in vivo correlation
Research Article
10.3897/pharmacia.67.e48896
2020-07-31
pharmacia
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Logoyda, Liliya
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Herasymiuk, Maksym
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Popovych, Dariya
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Pidruchna, Svitlana
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Hlushok, Vitaliy
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Herasymiuk, Nazar
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Zarivna, Nadiya
2020-07-31
2020-07-31
2020
Pharmacia
2603-557X
0428-0296
67
63-69
2020
10.3897/pharmacia.67.e48896
https://pharmacia.pensoft.net/article/48896/
https://pharmacia.pensoft.net/article/48896/download/pdf/
https://pharmacia.pensoft.net/article/48896/download/xml/
Aim. An understanding of the role that transporters, in particular P-glycoprotein (P-gp), can play in the absorption, distribution, metabolism and excretion (ADME) of candidate drugs, and an assessment of how these processes might impact on toxicity and the potential for drug-drug interactions in the clinic, is required to support drug development and registration. It is therefore necessary to validate preclinical assays for the in vitro evaluation of candidate drugs as substrates or inhibitors of human P-gp. 2. A simple, rapid HPLC MS/MS method was developed for determination of verapamil hydrochloride from confluent Caco-2 monolayers and from aqueous solution. Materials and methods. Chromatography was achieved on Discovery C18, 50 × 2.1 mm, 5 μm column. Samples were chromatographed in a gradient mode (eluent A (acetonitrile – water – formic acid, 5 : 95 : 0.1 v/v), eluent B (acetonitrile – formic acid, 100 : 0.1 v/v)). The initial content of the eluent B is 0%, which increases linearly by 1.0 min to 100% and to 1.01 min returns to the initial 0%. The mobile phase was delivered at a flow rate of 0.4 mL/min into the mass spectrometer ESI chamber. The sample volume was 5 μl. Results. Under these conditions, verapamil hydrochloride was eluted at 1.08 min. A linear response function was established at 1 – 100 ng/mL. The regression equation for the analysis was Y = 0.0162x + 0.00391 with coefficient of correction (R2) = 0.9992. According to the Caco-2 test results, verapamil showed low permeability. It should be noted that the recovery value for verapamil hydrochloride is 102.69%. The within-run coefficients of variation ranged between 0.336% and 0.617% for verapamil. The within-run percentages of nominal concentrations ranged between 98.82% and 100.62% for verapamil. The between-run coefficients of variation ranged between 0.334% and 0.612% for verapamil. The between-run percentages of nominal concentrations ranged between 98.97% and 101.76% for verapamil. The assay values on both the occasions (intra- and inter-day) were found to be within the accepted limits. Conclusion. From results of analysis, it can be concluded that developed method is simple and rapid for determination of verapamil hydrochloride from confluent Caco-2 monolayers and from aqueous solution. Acquired results demonstrate that proposed strategy can be effortlessly and advantageously applied for examination of verapamil hydrochloride from Caco-2 cell monolayers.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
HPLC-MS/MS
Verapamil hydrochloride
Caco-2 cells
Recovery
Bioavailability
HPLC MS/MS method development for the quantitative determination of verapamil hydrochloride from Caco-2 cell monolayers
Research Article
10.3897/pharmacia.67.e48319
2020-07-31
pharmacia
Medical University of Sofia, Sofia, Bulgaria
author
Kozuharova, Ekaterina
https://orcid.org/0000-0001-6795-9660
Medical University of Sofia, Sofia, Bulgaria
author
Benbassat, Niko
https://orcid.org/0000-0001-8876-2728
Institute of Biodiversity and Ecosystem Research at the Bulgarian Academy of Sciences, Sofia, Bulgaria
author
Berkov, Strahil
Medical University of Sofia, Sofia, Bulgaria
author
Ionkova, Iliana
2020-07-31
2020-07-31
2020
Pharmacia
2603-557X
0428-0296
67
71-81
2020
10.3897/pharmacia.67.e48319
https://pharmacia.pensoft.net/article/48319/
https://pharmacia.pensoft.net/article/48319/download/pdf/
https://pharmacia.pensoft.net/article/48319/download/xml/
The high tolerance of various habitat conditions and potent propagation ability of Ailanthus altissima (Mill.) Swingle (Simaroubaceae) and Amorpha fruticosa L. (Fabaceae) promote their aggressive invasive behaviour. Additionally, they not only over-compete the local vegetation but suppress the seed development. In the newly invaded habitats they might not have suitable herbivores to control their populations. The aim of this review is to evaluate the potential of A. altissima and A. fruticosa, as cheap sources of valuable essential oils. The essential oils yield and compostion of both plant species vary significantly depending on plant parts, origin and time of collection. The main constituents of A. altissima essential oil are α-curcumene, α-gurjunene, γ-cadinene, α-humulene β-caryophyllene caryophyllene oxide, germacrene D etc. The main constituents of A. fruticosa are δ-cadinene, γ-cadinene, β-caryophyllene γ-muurolene +, ar-curcumene, myrcene etc. These essential oils have been reported to possess different activities such as antimicrobial, insect repellent, insecticidal and herbicidal activity. Due to the fact that these are aggressive invasive species, they can provide abundant and cheap resources. Additionally, future industrial exploitation of the biomass of these invasive plants for essential oils’ extraction might contribute to biodiversity conservation by relieving their destructive impact on the natural habitats.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
essential oils
pharmacological activity
invasive plants
Ailanthus altissima Amorpha fruticosa
Ailanthus altissima and Amorpha fruticosa – invasive arboreal alien plants as cheap sources of valuable essential oils
Review Article
10.3897/pharmacia.67.e50159
2020-07-31
pharmacia
I. Horbachevsky Ternopil State Medical University, Ternopil, Ukraine
author
Logoyda, Liliya
2020-07-31
2020-07-31
2020
Pharmacia
2603-557X
0428-0296
67
83-88
2020
10.3897/pharmacia.67.e50159
https://pharmacia.pensoft.net/article/50159/
https://pharmacia.pensoft.net/article/50159/download/pdf/
https://pharmacia.pensoft.net/article/50159/download/xml/
Aim. Poorly water-soluble drugs such as nifedipineoffer challenging problems in drug formulation as poor solubility is generally associated with poor dissolution characteristics and thus with poor oral bioavailability (BCS class II drugs). Methods of quantitative determination of nifedipine by methods of spectrophotometry and chromatography are described in the scientific literature. However, methods are not developedfor examination of nifedipine from Caco-2 cell monolayers. Caco-2 cell monolayers have been extensively used for years as a tool to test permeability, assess the oral absorption potential and study the absorption mechanism of compounds. Therefore, the aim of this study was to develop and validate an efficient HPLC MS/MS method for determination of nifedipine from Caco-2 cell monolayers. Materials and methods. Chromatography was achieved on DiscoveryC18, 50 × 2.1 mm, 5 μm column. Samples were chromatographed in a gradient mode (eluent A (acetonitrile – water – formic acid, 5 : 95 : 0.1 v/v), eluent B (acetonitrile – formic acid, 100 : 0.1 v/v)). The initial content of the eluent B is 0%, which increases linearly by 1.0 min to 100% and to 1.01 min returns to the initial 0%. The mobile phase was delivered at a flow rate of 0.4 mL/min into the mass spectrometer ESI chamber. The sample volume was 5 μl. Results. Under these conditions, nifedipine was eluted at 1.83 min. A linear response function was established at 1 – 100 ng/mL. The regression equation for the analysis was Y = 0.0323x-0.00121 with coefficient of correction (R2) = 0.9987. According to the Caco-2 test results, nifedipine showed high permeability. The within-run coefficients of variation ranged between 0.331% and 0.619% for nifedipine. The within-run percentages of nominal concentrations ranged between 98.80% and 100.63% for nifedipine. The between-run coefficients of variation ranged between 0.332% and 0.615% for nifedipine. The between-run percentages of nominal concentrations ranged between 98.98% and 101.71% for nifedipine. The assay values on both the occasions (intra- and inter-day) were found to be within the accepted limits. Conclusion. From results of analysis, it can be concluded that developed method is simple and rapid for determination of nifedipine from confluent Caco-2 monolayers and from aqueous solution. Acquired results demonstrate that proposed strategy can be effortlessly and advantageously applied for examination of nifedipine from Caco-2 cell monolayers.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
LC-MS/MS
Nifedipine
Caco-2 cells
Recovery
Bioavailability
HPLC-MS/MS method development for the quantitative determination of nifedipine for Caco-2 permeability assay
Research Article
10.3897/pharmacia.67.e48472
2020-07-31
pharmacia
Medical University of Varna, Varna, Bulgaria
author
Gugleva, Viliana
Medical University of Varna, Varna, Bulgaria
author
Zasheva, Stanimira
Medical University of Varna, Varna, Bulgaria
author
Hristova, Maya
Medical University of Varna, Varna, Bulgaria
author
Andonova, Velichka
https://orcid.org/0000-0002-7369-1506
2020-07-31
2020-07-31
2020
Pharmacia
2603-557X
0428-0296
67
89-94
2020
10.3897/pharmacia.67.e48472
https://pharmacia.pensoft.net/article/48472/
https://pharmacia.pensoft.net/article/48472/download/pdf/
https://pharmacia.pensoft.net/article/48472/download/xml/
Resveratrol is a natural polyphenolic phytoalexin found in grapes, berry skins, roots of Japanese knotweed and is reputed as an excellent antioxidant, anti-inflammatory, neuro- and cardio- protective agent. Resveratrol has also beneficial effects in therapy of different skin conditions such as acne, exfoliative eczema, psoriasis and is known to provide a protection against ultraviolet radiation-mediated oxidative stress. However, its low oral bioavailability and short biological half- life compromise its beneficial therapeutic effects; therefore, its topical application is a practical approach in the treatment of various cutaneous disorders. Challenges associated with the development of topical resveratrol drug delivery systems and dosage forms include its low aqueous solubility as well as its poor UV-, pH- and temperature-dependent stability. The purpose of this article is to discuss the mechanism of action, therapeutic effect and physicochemical properties of resveratrol and to present recent technological approaches designed to improve its stability, bioavailability and therapeutic efficiency.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
antioxidant activity
biologically active compound
drug delivery systems
skin protection
Topical use of resveratrol: technological aspects
Review Article
10.3897/pharmacia.67.e50390
2020-07-31
pharmacia
Medical University of Sofia, Sofia, Bulgaria
author
Popova, Pavlinka
Medical University of Sofia, Sofia, Bulgaria
author
Zarev, Yancho
https://orcid.org/0000-0001-5830-4697
Medical University of Sofia, Sofia, Bulgaria
author
Shkondrov, Aleksandar
https://orcid.org/0000-0001-5091-6058
Medical University of Sofia, Sofia, Bulgaria
author
Krasteva, Ilina
https://orcid.org/0000-0001-8559-0369
Medical University of Sofia, Sofia, Bulgaria
author
Ionkova, Iliana
2020-07-31
2020-07-31
2020
Pharmacia
2603-557X
0428-0296
67
95-99
2020
10.3897/pharmacia.67.e50390
https://pharmacia.pensoft.net/article/50390/
https://pharmacia.pensoft.net/article/50390/download/pdf/
https://pharmacia.pensoft.net/article/50390/download/xml/
Establishment of in vitro cultures from Astragalus glycyphyllos, determination of biomass and analysis of total flavonoids, rutin and camelliaside A were performed. To increase flavonoid production various combinations of plant hormones and light/dark regimen were investigated. Suspension cultures with exogenous quercetin were evaluated for possible increase in flavonoid production. Shoots, calli and suspensions were successfully established. Rutin and camelliaside A were proved in highest amount in shoots. Calli, cultivated on modified G48 medium, with double amount of Ca2+ and Mg2+, achieved higher total flavonoid content (2.37 and 2.03 mg/g DW). Suspensions cultures, cultivated on modified G48 medium with 10, 20 and 30 mg/mL quercetin achieved higher total flavonoid content (0.09, 0.10 and 0.13 mg/mg DW). Biotransformation of quercetin to isoquercitrin was achieved. The highest concentration of isoquercitrin (56.73 ng/mg DW) was observed on suspensions cultures cultivated on modified G48 medium, with 20 mg/mL quercetin.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Astragalus glycyphyllos
flavonoids
in vitro cultures
Induction of flavonoid biosynthesis by in vitro cultivation of Astragalus glycyphyllos L.
Research Article
10.3897/pharmacia.67.e39045
2020-07-31
pharmacia
Medical University of Plovdiv, Plovidv, Bulgaria
author
Becheva, Maria
https://orcid.org/0000-0002-2734-8280
2020-07-31
2020-07-31
2020
Pharmacia
2603-557X
0428-0296
67
101-103
2020
10.3897/pharmacia.67.e39045
https://pharmacia.pensoft.net/article/39045/
https://pharmacia.pensoft.net/article/39045/download/pdf/
https://pharmacia.pensoft.net/article/39045/download/xml/
The purpose of the paper is to prove the efficacy of a kinesitherapeutic program intherecovery of a patient with trimalleolar fractureofthe left lower limb.We present a 42-year-old patient with a trimalleolar fracture and paresis of n. peroneus communis. Kinesitherapy was performed after thirteen weeks of surgery for ten consecutive days. The kinesitherapeuticmeans included: cryotherapy, lymphatic drainage massage, isometric contractions for the femur muscles, active exercises with and without resistance, active exercises with and on gymnastic apparatuses, post-isometric relaxation for the sciatic musculature and m. triceps surai, training in walking.As a result of the kinesitherapeutic exercises, the ankle swelling decreased.The volume of movement increased, pain was reduced as well as the muscle spasm in the ankle joint. The gait improved.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
kinesitherapy
trimalleolar fracture
Kinesitheraphy in trimalleolar fracture – case report
Research Article
10.3897/pharmacia.67.e55257
2020-08-06
pharmacia
National Center of Public Health and Analyses, Sofia, Bulgaria
author
Petrova, Svetla
National Center of Public Health and Analyses, Sofia, Bulgaria
author
Christova-Bagdassarian, Valentina
https://orcid.org/0000-0002-1485-6358
2020-08-06
2020-08-06
2020
Pharmacia
2603-557X
0428-0296
67
105-110
2020
10.3897/pharmacia.67.e55257
https://pharmacia.pensoft.net/article/55257/
https://pharmacia.pensoft.net/article/55257/download/pdf/
https://pharmacia.pensoft.net/article/55257/download/xml/
Sweeteners are substances used as a dietary supplement to replace sugar. Consumers are concerned about the high levels of sugar, calories and cariogenicity in confectionery products, which is why the popularity of the so-called. „Light“ products and „sugar-free“ products. Acesulfame K is a synthetic sweetener about 200 times sweeter than sugar. In the present work, an analysis of acesulfame K in cocoa and chocolate products was performed. For the determination of sweeteners acesulfame K, saccharin and aspartame in foodstuffs, a standardized reverse phase high performance liquid chromatography method with UV detection was used. A cocoa matrix-specific compound was observed in all chocolate products analyzed for acesulfame K. Interference did not correspond to acesulfame K on the UV spectrum and could not be removed by two-step purification. The comparison of the spectral characteristics allowed to avoid a misleading result for the presence of acesulfame K in chocolate and cocoa products.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
chocolate and cocoa products
Е 950
HPLC – UV
synthetic sweeteners
Analytical difficulties for determination of acesulfame K in chocolate products
Research Article
10.3897/pharmacia.67.e50806
2020-08-20
pharmacia
Medical University of Sofia, Sofia, Bulgaria
author
Ilieva, Viktoria
https://orcid.org/0000-0001-7428-602X
Medical University of Sofia, Sofia, Bulgaria
author
Kondeva-Burdina, Magdalena
National Center of Public Health and Analyses, Sofia, Bulgaria
author
Georgieva, Tzveta
https://orcid.org/0000-0002-3066-9448
2020-08-20
2020-08-20
2020
Pharmacia
2603-557X
0428-0296
67
111-114
2020
10.3897/pharmacia.67.e50806
https://pharmacia.pensoft.net/article/50806/
https://pharmacia.pensoft.net/article/50806/download/pdf/
https://pharmacia.pensoft.net/article/50806/download/xml/
In this study, we investigated the hypothesis that an additional source of free radicals may be hydrogen peroxide formed by monoamino oxidase (MAO) -catalyzed deamination of catecholamines. Also, increased MAO-B activity in the brain has been linked to the development of some neurodegenerative diseases. The toxicant we used to treat recombinant human MAO-B enzyme is the cyanotoxin аnatoxin-a. For anatoxin-a is known that it’s an agonist of neuronal acetylcholine receptors with 20 times greater affinity to them compared to the natural neurotransmitter. In this study, we analyzed the effect of anatoxin at various selected concentrations on the activity of recombinant human MAO-B enzyme. The method we use is to analyze the activity of human MAO-B with the fluorimetric reagent Amplex UltraRed and the substrate tyramine hydrochloride. The aim of this study is to analyze the effect of anatoxin-a on the hMAO-B enzyme activity and its influence as a factor for the development and progression of neurodegenerative diseases.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
MAO-B activity
neurodegenerative diseases
anatoxin-a
In vitro analysis of the activity of human monoamine oxidase type B (hMAOB), treated with the cyanotoxin anatoxin-a: supposed factor of neurodegenerative diseases
Research Article
10.3897/pharmacia.67.e52738
2020-08-25
pharmacia
"Sveta Ekaterina" Hospital, Sofia, Bulgaria
author
Kyuchukov, Dimitar
https://orcid.org/0000-0003-2442-7095
Tokuda, Sofia, Bulgaria
author
Zheleva-Kyuchukova, Ivayla
https://orcid.org/0000-0002-4644-3531
Sveta Ekaterina Hospital, Sofia, Bulgaria
author
Nachev, Gencho
2020-08-25
2020-08-25
2020
Pharmacia
2603-557X
0428-0296
67
115-120
2020
10.3897/pharmacia.67.e52738
https://pharmacia.pensoft.net/article/52738/
https://pharmacia.pensoft.net/article/52738/download/pdf/
https://pharmacia.pensoft.net/article/52738/download/xml/
Background: Coronary artery bypass grafting (CABG) remains the gold standard in the treatment of complex chronic forms of coronary heart disease (CHD). Coronary endarterectomy (CEAE) is a useful adjunctive technique to CABG in patients with diffuse coronary artery disease. In order to maintain the patency of the coronary arteries and graft conduits, various antithrombotic protocols have been introduced over the years, combining various antiplatelet and anticoagulant drugs, but still there is no consensus. Aim: The aim of the study is to compare results between two antithrombotic regimens after CEAE. The first one is a combination of acenocoumarol combined with acetylsalicylic acid (ASA), the second regimen is a dual antiplatelet therapy (DAPT) of clopidogrel combined with ASA. Material and methods: We retrospectively reviewed 56 consecutive patients (60 ± 8.2 years) undergoing isolated CABG in association with CEAE between January 2018 and December 2019. In the postoperative period, patients were divided into two groups according to the antithrombotic regimens described above. Twenty-four were in the ASA and acenocoumarol group (AA) and 32 were in the ASA and clopidogrel group (AC). Patients were followed up to 30 days after the operation and we access the mortality rate, new ECG changes, levels of myocardial fraction of creatinine phosphokinase (CPK-MB), left ventricular systolic function, pericardial or pleural effusions requiring drainage or revision for bleeding. Results: Operative mortality was 3,6 %. No differences in the antithrombotic efficacy of the two regimens. A significantly higher level of hemorrhagic complications was observed in the ASA + acenocoumarol treatment group. Conclusion: Dual antiplatelet therapy (DAPT) after CABG and coronary endarterectomy is an effective pharmacological regimen in regard to 30-day postoperative outcomes and is considerably safety in terms of bleeding complications.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
dual antiplatelet therapy (DAPT)
coronary endarterectomy (CEAE)
coronary artery bypass grafting (CABG)
Antithrombotic regimens in patients after coronary artery bypass grafting and coronary endarterectomy
Research Article
10.3897/pharmacia.67.e53886
2020-08-28
pharmacia
Institute of Cryobiology and Food Technologies, SOFIA, Bulgaria
author
Miteva, Daniela
Institute of Cryobiology and Food Technologies, SOFIA, Bulgaria
author
Solak, Ayten
Institute of Cryobiology and Food Technologies, SOFIA, Bulgaria
author
Dyankova, Svetia
Institute of Cryobiology and Food Technologies, SOFIA, Bulgaria
author
Nacheva, Iliana
Institute of Cryobiology and Food Technologies, SOFIA, Bulgaria
author
Dimov, Krasimir
2020-08-28
2020-08-28
2020
Pharmacia
2603-557X
0428-0296
67
129-133
2020
10.3897/pharmacia.67.e53886
https://pharmacia.pensoft.net/article/53886/
https://pharmacia.pensoft.net/article/53886/download/pdf/
https://pharmacia.pensoft.net/article/53886/download/xml/
The objective of the present in vivo study was to analyze the changes in the immune response of the sensitized Ваlb/c mice against milk allergens in lyophilized cow’s milk (LCM) and whey curd (LC) before and after gamma irradiation (10 kGy). The results showed lower levels of IgE in the group treated with irradiated LCM (ICM) compared to the group treated with non-irradiated LCM (NiCM). Hence, it could be suggested that gamma irradiation influenced the epitopes of the major milk proteins and was associated with lower allergenicity of the lyophilized irradiated milk. The gamma irradiation in the whey curd, however, did not significantly change the level of IgE antibodies in IC (treated with irradiated LC) compared to NiC (treated with non-irradiated LC) group.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Cow’s milk hypersensitivity
murine model
irradiation
IgE
ELISA
Assessment of allergenicity of irradiated dairy products in a Balb/c mice model
Research Article
10.3897/pharmacia.67.e38969
2020-08-28
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Chaban, Taras
https://orcid.org/0000-0003-0618-275X
Ivan Franko National University of Lviv, Lviv, Ukraine
author
Matiychuk, Vasyl
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Komarytsya, Olexandra
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Myrko, Iryna
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Chaban, Ihor
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Ogurtsov, Volodymyr
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Nektegaev, Ihor
2020-08-28
2020-08-28
2020
Pharmacia
2603-557X
0428-0296
67
121-127
2020
10.3897/pharmacia.67.e38969
https://pharmacia.pensoft.net/article/38969/
https://pharmacia.pensoft.net/article/38969/download/pdf/
https://pharmacia.pensoft.net/article/38969/download/xml/
Synthesis of novel N3 and C5 substituted thiazolo[4,5-b]pyridin-2-ones was carried out on the basis of [3+3]-cyclocodensation, acylation and alkylation reactions. The structures of the obtained compounds were confirmed by 1H NMR spectroscopy, and elemental analysis. The anti-inflammatory action of novel thiazolo[4,5-b]pyridine-2-one derivatives was evaluated in vivo employing the carrageenan-induced rat paw edema method. When compared with Ibuprofen, some our compounds were found to be more potent. Graphical abstract
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
anti-inflammatory activity
organic synthesis
thiazolo[4
5-b]pyridines
Anti-inflammatory properties of some novel thiazolo[4,5-b]pyridin-2-ones
Research Article
10.3897/pharmacia.67.e52585
2020-09-14
pharmacia
National University of Pharmacy, Kharkiv, Ukraine
author
Sarray, Dhurgham Khalid Abed Sarray
National University of Pharmacy, Kharkiv, Ukraine
author
Horiacha, Liliia
National University of Pharmacy, Kharkiv, Ukraine
author
Zhuravel, Iryna
National University of Pharmacy, Kharkiv, Ukraine
author
Fedosov, Andriy
2020-09-14
2020-09-14
2020
Pharmacia
2603-557X
0428-0296
67
145-152
2020
10.3897/pharmacia.67.e52585
https://pharmacia.pensoft.net/article/52585/
https://pharmacia.pensoft.net/article/52585/download/pdf/
https://pharmacia.pensoft.net/article/52585/download/xml/
Mirabilis jalapa is a popular decorative plant valued for its beautiful multicolored flowers. Folk medicine in various countries applies Mirabilis jalapa as anti-microbial, anti-inflammatory, diuretic, spasmolytic drug. Chemical composition of different types of Mirabilis jalapa has not yet been adequately studied which is an obstacle for its application in medicine. The qualitative composition and quantitative content of phenolic compounds were studied by the HPLC method. The performed experiment revealed presence of hydroxycinnamic acids, flavonoids, isoflavonoids and coumarins in tested herb. The content of phenolic compounds was the highest in Mirabilis jalapa flowers, counting as much as 2977.41 ± 59.55 µg/mg. Total content of phenolic compounds in Mirabilis jalapa herb was 304.25 ± 6.08 µg/mg, in fruits – 67.92 ± 1.36 µg/mg, and in roots – 12.44 ± 0.25 µg/mg. Quantitatively neochlorogenic acid dominated in flowers, chlorogenic acid in fruits, whereas Mirabilis jalapa herb mostly contained rutin and hyperoside. The obtained results will be useful in the development of quality control methods for Mirabilis jalapa herb and manufacture of drug preparations on its basis.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Mirabilis jalapa
four-o’clocks
phenolic compounds
HPLC
HPLC study of phenolic compounds in Mirabilis jalapa raw material
Research Article
10.3897/pharmacia.67.e51812
2020-09-14
pharmacia
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Savych, Alona
https://orcid.org/0000-0001-6053-7625
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Marchyshyn, Svetlana
Bukovina State Medical University, Chernivtsy, Ukraine
author
Basaraba, Roksolana
2020-09-14
2020-09-14
2020
Pharmacia
2603-557X
0428-0296
67
153-159
2020
10.3897/pharmacia.67.e51812
https://pharmacia.pensoft.net/article/51812/
https://pharmacia.pensoft.net/article/51812/download/pdf/
https://pharmacia.pensoft.net/article/51812/download/xml/
It was determined the qualitative composition and the quantitative content of fatty acids in the herbal antidiabetic collection № 3, № 4, № 7, № 13, № 19, which are used in folk medicine for prevention and treatment of diabetes mellitus type 2 in Ukraine by GC/MS method. According to the results it was identified 8 fatty acids in the herbal antidiabetic collection № 3, 13 fatty acids in the herbal antidiabetic collection № 4, 12 fatty acids in the herbal antidiabetic collection № 7, 13 fatty acids in the herbal antidiabetic collection № 13 and 11 fatty acids in the herbal antidiabetic collection № 19. The saturated fatty acids were found to be dominated in the raw plant material. The results of the quantitative research showed that the herbal antidiabetic collection № 3 contains 16.13 mg/g of fatty acids, the herbal antidiabetic collection № 4 – 27.17 mg/g, the herbal antidiabetic collection № 7 – 31.98 mg/g, the herbal antidiabetic collection № 13 – 27.37 mg/g, the herbal antidiabetic collection № 19 – 18.79 mg/g. The GC/MS analysis of the fatty acid composition in the herbal antidiabetic collections has shown that this raw material has a lot of fatty acids, which can have a positive effect for the treatment and prevention of diabetes mellitus type 2.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
diabetes mellitus
herbal antidiabetic collection
GC/MS
fatty acids
Determination of fatty acid composition content in the herbal antidiabetic collections
Research Article
10.3897/pharmacia.67.e52737
2020-09-14
pharmacia
MD, Sofia, Bulgaria
author
Zheleva-Kyuchukova, Ivayla
https://orcid.org/0000-0002-4644-3531
MD, Sofia, Bulgaria
author
Gelev, Valeri
2020-09-14
2020-09-14
2020
Pharmacia
2603-557X
0428-0296
67
135-143
2020
10.3897/pharmacia.67.e52737
https://pharmacia.pensoft.net/article/52737/
https://pharmacia.pensoft.net/article/52737/download/pdf/
https://pharmacia.pensoft.net/article/52737/download/xml/
The progress of percutaneous coronary interventions (PCI) over the last few decades facilitated treatment of increasingly complex patient populations. The introduction of drug-eluting stents (DESs) led to need of stronger and prolonged inhibition of platelets which in turn increased the incidence of bleeding complications. The identification and management of patients at high bleeding risk (HBR) during and after percutaneous coronary interventions (PCI) is still problematic in everyday clinical practice.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
percutaneous coronary interventions (PCI)
dual antiplatelet therapy (DAPT)
high bleeding risk (HBR)
acetylsalicylic acid (ASA)
Antiplatelet therapy after PCI in patients with high risk of bleeding
Review Article
10.3897/pharmacia.67.e52474
2020-09-15
pharmacia
Istitute of Neurobiology, Sofia, Bulgaria
author
Kadinov, Boris
Istitute of Neurobiology, Sofia, Bulgaria
author
Itzev, Dimitar
2020-09-15
2020-09-15
2020
Pharmacia
2603-557X
0428-0296
67
161-168
2020
10.3897/pharmacia.67.e52474
https://pharmacia.pensoft.net/article/52474/
https://pharmacia.pensoft.net/article/52474/download/pdf/
https://pharmacia.pensoft.net/article/52474/download/xml/
The interaction between carbon monoxide and nitric oxide and their role in modulation of stomach fundus excitability was studied. The presence and colocalization of heme oxygenase 1 (HO-1) and nitric oxide synthase (NOS) was verified in myentheric ganglia by immunohistochemistry. The role of inducible heme oxygenase isoenzyme was investigated after in vivo treatment of animals with CoCl2 (80 mg kg-1 b.w.) injected subcutaneously 24 hours before euthanasia. This treatment resulted in positive staining for the inducible isoform in stomach smooth muscle.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Contraction
guinea-pig
hemin
heme oxygenase
histochemistry
smooth muscle
Influence between NO and CO in guinea pig stomach fundus
Research Article
10.3897/pharmacia.67.e35080
2020-09-15
pharmacia
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Avakov, Valentyn
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Oktysyuk, Yuriy
https://orcid.org/0000-0001-7741-2490
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Rozhko, Mykola
2020-09-15
2020-09-15
2020
Pharmacia
2603-557X
0428-0296
67
169-172
2020
10.3897/pharmacia.67.e35080
https://pharmacia.pensoft.net/article/35080/
https://pharmacia.pensoft.net/article/35080/download/pdf/
https://pharmacia.pensoft.net/article/35080/download/xml/
To be living at the most advanced technological area in history is to exist and face pollution one of the most serious threats to all living things on the planet. There is evidence that anthropogenic and technological pollutions cause a considerable number of health problems and stomatological in particular. This article demonstrates the results gained by administration of vitamin-macro element complex “Calcinova” and sorbent “Enterosgel” in correction of enzymes responsible for biotransformation of xenobiotics in blood serum of children from the polluted areas. It has been established the high efficiency of recommended scheme on caries prophylaxis that is proved by positive biochemical indexes in blood serum of this children observed 30 month after the beginning of the investigation. As a result of the introduced prophylaxis, we have noticed the significant improvement of biochemical indexes in children with tooth decay. The findings reflect a certain rise in activity of enzymes responsible for elimination of xenobiotics such as alcohol-dehydrogenase (ADH) and glutathione-S-transferase (GST) obtained from the blood serum of children from the main group comparing to the control group.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
dental caries
xenobiotics
alcohol dehydrogenase
glutathione-S-transferase
prophylaxis
Prophylaxis of dental caries in children from polluted regions
Research Article
10.3897/pharmacia.67.e56788
2020-10-02
pharmacia
Universitas Gadjah Mada, Yogyakarta, Indonesia
author
fakhrudin, nanang
https://orcid.org/0000-0001-7954-9503
Institut Ilmu Kesehatan Bhakti Wiyata, Kediri, Indonesia
author
Pertiwi, Krisna
Politeknik Kesehatan Kemenkes Kupang, Kupang, Indonesia
author
Takubessi, Marce
https://orcid.org/0000-0002-5089-5143
Sekolah Tinggi Ilmu Kesehatan Borneo Lestari, Banjarbaru, Indonesia
author
Susiani, Eka
Universitas Gadjah Mada, Yogyakarta, Indonesia
author
Nurrochmad, Arief
https://orcid.org/0000-0001-7597-2574
Universitas Gadjah Mada, Yogyakarta, Indonesia
author
Widyarini, Sitarina
Universitas Gadjah Mada, Yogyakarta, Indonesia
author
Sudarmanto, Ari
Universitas Gadjah Mada, Yogyakarta, Indonesia
author
Nugroho, Arief
Universitas Gadjah Mada, Yogyakarta, Indonesia
author
Wahyuono, Subagus
2020-10-02
2020-10-02
2020
Pharmacia
2603-557X
0428-0296
67
173-180
2020
10.3390/molecules21091114
10.1345/aph.1M548
10.1161/CIR.0000000000000659
10.1111/jth.12952
10.1111/jth.12231
10.1063/1.5062741
10.1007/s11418-006-0048-0
10.1016/j.jep.2010.03.031
10.3109/13880200903008641
10.1007/s11418-010-0410-0
10.1038/sj.bjp.0703046
10.3390/ijms18081803
10.3389/fphar.2018.00037
10.1016/j.jacc.2005.02.092
10.1007/s11239-020-02098-4
10.1007/s11239-011-0558-9
10.1016/j.thromres.2009.04.003
10.1038/nature06797
10.1038/s41573-020-0061-0
10.3390/ijms21041391
10.1002/(SICI)1097-458X(199609)34:9%3C719::AID-OMR948%3E3.0.CO;2-Q
10.2741/1939
10.2147/TCRM.S19835
10.1111/bph.13429
10.3109/10409239709082000
10.1002/cphy.c170049
10.1080/09537100120058739
10.3390/ijms19061578
10.1016/j.phytochem.2007.01.009
10.1016/j.phytochem.2006.01.030
10.1124/pr.117.014530
10.3897/pharmacia.67.e56788
https://pharmacia.pensoft.net/article/56788/
https://pharmacia.pensoft.net/article/56788/download/pdf/
https://pharmacia.pensoft.net/article/56788/download/xml/
Platelet plays a crucial role in cardiovascular diseases (CVDs) development. Abnormalities in platelet aggregation provokes thromboembolism, eventually leading to death. In Indonesia, breadfruit (Artocarpus altilis) leaf is traditionally used to treat CVDs. This study aimed to evaluate the antiplatelet activity of A. altilis leaf extract (AAE) and to identify its active compound. A. altilis leaves were extracted with ethanol, and the antiplatelet activity was assessed using ADP-induced platelet aggregation. The major compound was isolated with column chromatography followed by preparative TLC, and the structure was determined on the basis of UV, MS, IR, and NMR spectra. The binding mode of the active compound to platelet receptors was characterized in in silico study. AAE exhibited an antiplatelet activity (IC50 of 252.23 µg/mL). A geranylated chalcone, 2-geranyl-2ʹ,3,3,4ʹ-tetrahydroxydihydrochalcone (GTDC) was identified as the antiplatelet compound (IC50 of 9.09 µM). GTDC actions with P2Y12 platelet receptor involving three amino acid residues.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Artocarpus communis
2-geranyl-2ʹ
3
3
4ʹ-tetrahydroxydihydrochalcone
thrombosis
P2Y12
P2Y1
A geranylated chalcone with antiplatelet activity from the leaves of breadfruit (Artocarpus altilis)
Research Article
10.3897/pharmacia.67.e46586
2020-10-02
pharmacia
National University of Pharmacy, Kharkiv, Ukraine
author
Kotvitska, Alla
National University of Pharmacy, Kharkiv, Ukraine
author
Prokopenko, Oleksii
https://orcid.org/0000-0002-9095-4160
2020-10-02
2020-10-02
2020
Pharmacia
2603-557X
0428-0296
67
215-221
2020
World Health Organization. WHO Guideline on Country Pharmaceutical Pricing Policies. https://www.ncbi.nlm.nih.gov/books/NBK258631/
World Health Organization. World Health Assembly. (n.d.). https://www.who.int/about/governance/world-health-assembly. [Published May 29, 2019]
World Health Organization. WHO Roadmap for access 2019–2023. (20–28 of May 2019). https://www.who.int/medicines/access_use/Roadmap_for_access_zero_draft.pdf
10.1002/mds.25945
10.1503/cmaj.151179
National Institute for Health and Care Excellence. Parkinson’s disease in adults. www.nice.org.uk/guidance/ng71
Republican Center for Health Development. Clinical protocol of diagnosis and treatment of Parkinson’s disease. www.rcrz.kz/docs/clinic_protocol/2016/2%D0%BF%D0%B3/
Clinical protocol for the provision of medical care to patients with Parkinson’s disease. https://zakon.rada.gov.ua/rada/show/v0487282-07/
Morion Company. Research of the pharmaceutical market of Ukraine, analytical company “Pharmstandard" of Morion Company Ukraine. http://www.pharmstandart.com.ua/login.
I
Kubarieva
author
2010
2010
State Statistics Service of Ukraine. Average monthly salary for regions. http://www.ukrstat.gov.ua/operativ/operativ2005/gdn/reg_zp_m/reg_zpm_u/arh_zpm_u.htm
State Statistics Service of Ukraine. Average monthly retirement contribution and number of pensioners. http://www.ukrstat.gov.ua/operativ/operativ2007/sz/sz_u/srp_07rik_u.html
CMU Resolution No. 955 from 17.10.2008 “Measures to stabilize the prices of medicines and medical products". https://zakon.rada.gov.ua/laws/main/184-2019-%D0%BF
10.1111/j.1475-2743.2009.00228.x
CMU Resolution No. 1303 of 17.08.1998 “About regulation of free and preferential dispensing of the prescribed medicines in the case of outpatient treatment of certain populations and for certain categories of diseases". https://zakon.rada.gov.ua/laws/main/1303-98-%D0%BF
World Health Organization. WHO Model Lists of Essential Medicines. https://www.who.int/medicines/publications/essentialmedicines/en/
10.3897/pharmacia.67.e46586
https://pharmacia.pensoft.net/article/46586/
https://pharmacia.pensoft.net/article/46586/download/pdf/
https://pharmacia.pensoft.net/article/46586/download/xml/
In the context of the economic crisis, the availability of medicines for the population is one of the key issues facing pharmaceutical sector of healthcare system. Due to the fact of that, the purpose of the work was to study the social and economic accessibility of medicines, since such studies in the future may provide an opportunity for effective input of medicines costs reimbursement system, which will increase their availability, especially for socially vulnerable groups of population. The data analysis of the clinical protocols of Great Britain, Kazakhstan and Ukraine was conducted, and the indicators of social and economic availability within 2014–2018 were calculated. The obtained results indicate an adequate level of availability of medicines for the able-bodied population and, unfortunately, a low level of accessibility of medicines for people of retirement age with a tendency to further descension. These results point the necessity of the further research and development of government control systems and provision of pharmaceutical care for the population.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
clinical protocol
medicines
index of retail prices
neurological diseases
Determination of social and economic accessibility of drugs for treatment of Parkinson’s disease on the basis of modern approaches
Research Article
10.3897/pharmacia.67.e50458
2020-10-02
pharmacia
Medical University of Plovdiv, Plovdiv, Bulgaria
author
Mihaylova, Anna A.
https://orcid.org/0000-0002-7674-6074
Medical University of Plovdiv, Plovdiv, Bulgaria
author
Kasnakova, Petya
Medical University of Plovdiv, Plovdiv, Bulgaria
author
Gueorguiev, Stanislav
Medical University of Plovdiv, Plovdiv, Bulgaria
author
Petkova-Gueorguieva, Elina S.
https://orcid.org/0000-0003-1488-3863
Medical University Sofia, Sofia, Bulgaria
author
Peikova, Lily
https://orcid.org/0000-0002-8030-3479
2020-10-02
2020-10-02
2020
Pharmacia
2603-557X
0428-0296
67
209-214
2020
RA
Ballard
author
2006
2006
10.1016/S0140-6736(12)60820-4
2013
2013
Brazilian Ministry of Health (2013) SINASC – National Information System on Live Births, 2013 Nov 27.
10.1016/S0029-7844(97)00047-1
10.1016/0167-6296(91)90031-H
10.1371/journal.pmed.1001502
10.3325/cmj.2015.56.119
10.1016/S0029-7844(01)01732-X
10.1016/0002-9378(95)90222-8
10.1002/14651858.CD003935.pub3
Effects of antenatal corticosteroids on maternal serum indicators of infection in women at risk for preterm delivery: A randomized trial comparing betamethasone and dexamethasone.
A
Danesh
author
2012
text
Journal of Research in Medical Science
2012
17
911
7
10.1016/S0029-7844(03)00485-X
2010
2010
EURO-PERISTAT (2010) Project, with SCPE, EUROCAT, EURONEOAT. European Perinatal Health Report. [Accessed: April 1, 2015]
10.1016/j.ajog.2006.09.014
10.1016/S0002-9378(99)70160-2
10.1016/S0140-6736(08)60074-4
Births: Preliminary data for 2010.
BE
Hamilton
author
2011
text
National Vital Statistics Reports
2011
62
1
1
70
10.1111/j.1365-3016.2005.00644.x
, Deaths: leading causes for 2008.
M
Heron
author
2012
text
National Vital Statistics Reports
2012
60
6
1
94
CP
Howson
author
2012
2012
10.1016/S0140-6736(08)60108-7
Adequacy of prenatal care and pregnancy outcome.
PM
Krueger
author
2000
text
Journal of the American Osteopathic Association
2000
100
485
492
J
Martin
author
2012
2012
RL
Drake
author
2014
2014
KL
Moore
author
2002
2002
10.1016/j.jpeds.2010.12.039
10.1542/peds.112.6.1290
10.1046/j.1365-2214.2001.00203.x
10.1111/j.1471-0528.2005.00577.x
2015
2015
Preterm Labor and Birth: Condition Information (2015) http://www.nichd.nih.gov [03/11/2014. Retrieved 7 March 2015]
„Respiratory distress syndrome and its management“.
RJ
Rodriguez
author
AA
Fanaroff
author
2002
text
St. Louis: Mosby
2002
1001
1011
2010
2010
RCOG [Royal College of Obstetricians and Gynaecologists] (2010) Antenatal corticosteroids to reduce neonatal morbidity and mortality. London (UK): Royal College of Obstetricians and Gynaecologists (RCOG);
M
Sandeva
author
2016
2016
S
Sinha
author
2012
2012
10.1111/j.1471-0528.2005.00575.x
10.3897/pharmacia.67.e50458
https://pharmacia.pensoft.net/article/50458/
https://pharmacia.pensoft.net/article/50458/download/pdf/
https://pharmacia.pensoft.net/article/50458/download/xml/
Preterm birth is a vital global health-economic problem. Health disorders provoked by it generate a high neonatal mortality rate. Prenatal corticosteroid prevention aims to reduce postnatal complications in premature infants. This survey covered two basic baby groups: work group of 89 premature infants that had been subjected to prenatal corticosteroid prophylaxis and a control group of 78 premature babies without prenatal prevention. The analysis of the pharmacoeconomic aspects of prenatal corticosteroid prevention enabled the comparison of clinical and therapeutic results, treatment costs, therapeutic expenditures, shortterm therapeutic effect, benefits and sequences from premature infants’ therapy. The analysis of clinical data obtained during this survey enabled the conclusion that when analyzing the combined effect of Dexamethasone prophylaxis, gestation week at birth and the age of the mother of premature infants with RDS, respiratory obstuction occurrence was mediated by the earlier gestation week at birth, older mother’s age and, at this background, it was restricted to a certain extent by prenatal corticosteroid administration. Conclusions: Prenatal corticosteroids cause reduction of premature infants’ treatment costs. The implementation of a smaller number of dexamethasone applications leads to smaller expenditures for premature infants’ treatment and care compared to those that have more dexamethasone applications.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
preterm birth
prenatal corticosteroid prophylaxis
health costs
premature neonates
RDS
Cost analysis of neonates after prenatal corticosteroid prophylaxis of Respiratory Distress Syndrome
Research Article
10.3897/pharmacia.67.e55835
2020-10-02
pharmacia
Medical University, Sofia, Bulgaria
author
Chakuleska, Lidija
Medical University, Sofia, Bulgaria
author
Simeonova, Rumiana
https://orcid.org/0000-0003-4860-9053
Medical University, Sofia, Bulgaria
author
Danchev, Nikolay
2020-10-02
2020-10-02
2020
Pharmacia
2603-557X
0428-0296
67
199-207
2020
10.1016/j.bonr.2019.100216
10.1007/s11657-016-0286-z
10.1002/psb.1815
10.1136/bmj.i5109
AM
Borisova
author
2019
2019
10.1016/j.jbspin.2018.02.009
10.1007/s10198-019-01066-x
10.1359/jbmr.061113
10.1016/j.fct.2019.110668
Review on the pharmacology of osteoporosis.
LV
Chakuleska
author
2018
text
Pharmacia
2018
65
3
49
56
10.1007/s11657-017-0324-5
10.1016/j.jval.2017.02.006
10.1210/jc.2019-00221
2014
2014
EMA/10206/ (2014) PRAC recommends suspending use of Protelos/Osseor (strontium ranelate). https://www.ema.europa.eu/en/documents/press-release/prac-recommends-suspending-use-protelos/osseor_en.pdf
2013
2013
EMA/109665/2013 (2013) Assessment report for calcitonin containing medicinal products. https://www.ema.europa.eu/en/documents/referral/assessment-report-calcitonin-containing-medicinal-products_en.pdf
10.1007/s00198-005-1943-z
10.1007/978-3-030-33861-9_3
2020
2020
Health Insurance Fund of Republic of North Macedonia (2020) Health Insurance Fund of Republic of North Macedonia. http://www.fzo.org.mk/default-mk.asp
10.1007/s11657-013-0136-1
10.1007/s00198-018-4704-5
10.3389/fphar.2017.00288
2014
2014
Law for health insurance (2014) Law for health insurance, State Gazette 25/ March 2000, last amended State Gazette 113/ 2014.
2007
2007
Law on medicine and medical devices (2007) Law on medicine and medical devices “Official gazette of RM” 106/ 05.09.2007
10.1002/jbm4.10192
2020
2020
Ministry Council (2020) Ministry Council, Decree on conditions, rules, and order of regulation and registration of prices of medicinal products. State Gazette 40/30 April 2013; last amended State Gazette 19/ 6 March 2020.
2020
2020
Ministry of Health of the Republic of Bulgaria (2020) Ministry of Health of the Republic of Bulgaria; Inclusion of medicinal products in the Positive Drug List of the Republic of Bulgaria. https://portal.ncpr.bg/registers/pages/register/list-medicament.xhtml
2020
2020
National Council on Prices and Reimbursement of Medicinal Products in R. Bulgaria (2020) National Council on Prices and Reimbursement of Medicinal Products in R. Bulgaria. https://portal.ncpr.bg/registers/pages/register/list-medicament.xhtml
2009–2020
2009–2020
National Health Insurance Fund of Republic of Bulgaria (2009–2020) National Health Insurance Fund of Republic of Bulgaria. https://www.nhif.bg
2018
2018
National Institutes of Health (2018) Osteoporosis and Related Bone Diseases. National Resource Center. Osteoporosis Overview. https://www.bones.nih.gov/sites/bones/files/pdfs/osteopoverview-508.pdf
2020
2020
National People Assembly (2020) National People Assembly. Law for health insurance, State Gazette 70/ 19 June, 1998, last amended State Gazette 54/ 16 June 2020
10.1007/s11739-018-1874-2
2008
2008
Official Gazette of the Republic of Macedonia (2008) Official Gazette of the Republic of Macedonia No. 8 dated 17.01.2008, 54–55, Rulebook on Determining Criteria and Procedure for Determining Reference Prices of Medicines. http://www.slvesnik.com.mk/Issues/1C913E2A48F32644A11C20606BE45627.pdf
10.1517/14740338.2014.939169
10.1097/00005131-200309001-00002
Pharmacotherapy costs of osteoporosis and related fractures in Bulgaria.
A
Savova
author
2012
text
Acta Medica Bulgarica (XXXIX)
2012
1
31
39
10.1210/clinem/dgaa048
10.5152/eurjrheum.2016.048
10.4103/2249-4863.94457
10.2147/TCRM.S3552
10.1007/s11657-014-0187-y
Our reflection on prices of parapharmaceuticals for osteoporosis prevention.
V
Vasilev
author
2003a
text
Macedonian Pharmaceutical Bulletin
2003a
49
1
2
Pricing approaches towards the parapharmaceuticals for osteoporosis prevention.
V
Vasilev
author
2003b
text
Macedonian Pharmaceutical Bulletin
2003b
49
1
2
1994
1994
World Health Organization (1994) Assessment of fracture risk and its application to screening for postmenopausal osteoporosis: report of a WHO study group [meeting held in Rome from 22 to 25 June 1992] https://apps.who.int/iris/handle/10665/39142
10.3897/pharmacia.67.e55835
https://pharmacia.pensoft.net/article/55835/
https://pharmacia.pensoft.net/article/55835/download/pdf/
https://pharmacia.pensoft.net/article/55835/download/xml/
The aim of the present study was to analyze and compare the number of health insured persons suffering from osteoporosis and antiosteoporotic drugs reimbursed by the National Health Insurance Funds (NHIF) in the Republic of Bulgaria (RBG) and Republic of North Macedonia (RNM) for the period 2015–2018. The reimbursement cost paid by the NHIF for the same period in both countries was surveyed. The results show an increase in both the number of patients with osteoporosis and the cost of the treatment. The most prescribed drug in R. Bulgaria is denosumab and it has the highest costs respectively, while in R.N. Macedonia the most prescribed and respectively the most expensive treatment is the bisphosphonate ibandronic acid, tablets of 150 mg. The trend of rising costs in both countries is likely to continue due to the increasing number of patients with osteoporosis. The number of patients and cost of pharmacotherapy in RNM and RBG are increasing but the reimbursement policy in RNM is more restrictive in terms of reimbursed medicines. On the other side, the RNM is with a higher level of reimbursement that might positively affect the cost of pharmacotherapy.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
bisphosphonate
Health Insurance Fund
reimbursement status
Pharmacotherapy costs and medicines reimbursement policies of osteoporosis in the Republic of Bulgaria and Republic of North Macedonia
Research Article
10.3897/pharmacia.67.e46865
2020-10-02
pharmacia
Medical University, Plovdiv, Bulgaria
author
Becheva, Maria
https://orcid.org/0000-0002-2734-8280
Medical University, Plovdiv, Bulgaria
author
Taneva, Daniela
2020-10-02
2020-10-02
2020
Pharmacia
2603-557X
0428-0296
67
181-185
2020
M
Becheva
author
2019
2019
10.1136/bmj.d2040
Osteoporosis-Diagnostic, Prevention and Treatement.
A-M
Borissova
author
1998
text
Endocrinolagia
1998
3
4
1
59
10.1053/ajkd.1998.v32.pm9808141
S
Ivanova
author
2015
2015
10.2174/1381612823666170714122714
SА
Ivanova
author
2019
2019
St
Ivanova
author
2017
2017
10.1007/s00198-004-1640-3
10.1002/jbmr.5650090802
10.1002/14651858.CD004143.pub4
10.1136/bmj.312.7041.1254
10.1001/jama.272.24.1942
10.1007/PL00022723
10.1007/s11657-012-0109-9
Role of phytoestrogens in prevention of osteoporosis.
St
Pankova
author
2015
text
International Journal of Current Pharmaceutical Research
2015
7
2
1
6
A review of current treatment options for osteporosis in Bulgaria.
St
Pankova
author
2015
text
World Journal of Pharmaceutical Sciences
2015
4
5
1
12
10.1210/er.2001-8002
Antioxidant characteristics of different varieties distributed in Bulgaria.
St
Papanov
author
2015
text
International Journal of Technical Research & Applications
2015
3
2
33
36
10.9734/JPRI/2017/35627
10.1001/jama.2010.594
10.1016/j.bone.2003.10.001
E
Seeman
author
1996
1996
10.1359/JBMR.050609
10.11648/j.ijnfs.s.2015040101.14
10.23958/ijirms/vol01-i04/04
Evaluation of separation of steroids in combined forms by RP HPLC with UV-detection and gas chromatography.
D
Tsvetkova
author
2017
text
Bulgarian Chemical Communications
2017
49
2
377
383
1994
Assessment of fracture risk and its application to screening for postmenopausal osteoporosis: report of a WHO study group. WHO Technical Report Series no.
1994
29 pp
World Health Organization (1994) Assessment of fracture risk and its application to screening for postmenopausal osteoporosis: report of a WHO study group. WHO Technical Report Series no.843WHO, Genève, 29 pp.
10.3897/pharmacia.67.e46865
https://pharmacia.pensoft.net/article/46865/
https://pharmacia.pensoft.net/article/46865/download/pdf/
https://pharmacia.pensoft.net/article/46865/download/xml/
Osteoporosis is a generalized bone disease characterized by diminished bone strength with an increased risk of fractures. Osteoporosis is a major health concern, both because of the risk of potentially serious fractures and because its prevalence is increasing as the population ages. The treatment of the disease is complex and includes adherence to a particular diet, medications (hormone replacement therapy, calcium, vitamin D, etc.), kinesitherapy, physiotherapy and orthotics. To reduce osteoporosis, it is necessary to avoid the risk of fractures, to do regular physical activities, to eat bone-healthy foods, to lead a proper lifestyle with the avoidance of negative habits and to maintain a healthy weight. A person also needs to have knowledge of personal risk factors and to consult a doctor if medical treatment is to be applied. The classic therapeutic approach is with antiresorptive anti-osteoporosis agents. A promising therapeutic trend is the study of compounds with low toxicity and potential properties to influence the pathogenetic mechanisms of disease and oxidative stress, such as phytoestrogens and hormone replacement therapy, at the same time. The aim of the article is to familiarize the audience with the preventive measures and therapies applied to the treatment of osteoporosis.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Osteoporosis
prophylaxis
medication treatment
Prevention and treatment of osteoporosis
Review Article
10.3897/pharmacia.67.e37433
2020-10-02
pharmacia
National University of Pharmacy, Kharkiv, Ukraine
author
Burda, Nadiia
https://orcid.org/0000-0002-7435-5731
National University of Pharmacy, Kharkiv, Ukraine
author
Zhuravel, Iryna
Middle East University, Amman, Jordan
author
Dababneh, Moeen F.
Ukrainian Scientific Pharmacopoeial Center for Quality of Medicines, Kharkiv, Ukraine
author
Kotov, Andrii
Ukrainian Scientific Pharmacopoeial Center for Quality of Medicines, Kharkiv, Ukraine
author
Kotova, Elina
National University of Pharmacy, Kharkiv, Ukraine
author
Popyk, Andrii
2020-10-02
2020-10-02
2020
Pharmacia
2603-557X
0428-0296
67
187-191
2020
10.4103/0973-7847.125530
10.1016/j.phytochem.2007.07.003
10.1016/j.phytochem.2013.04.005
10.1201/b10787
2016
2016
JP XVII [The Japanese Pharmacopoeia] (2016) Seventeenth Edition. Official From April 1, 2016 English Version The Ministry Of Health, Labour And Welfare. http://jpdb.nihs.go.jp/jp17e/000217650.pdf
10.1016/j.phytochem.2014.08.003
Comparative, qualitative and quantitative chemotypic characterization among North Indian Tribulus terrestris.
A
Kumar
author
2012
text
International Research Journal of Pharmacy
2012
3
6
212
218
Pharmacological scientific evidence for the promise of Tribulus terrestris.
J
Mohd
author
2012
text
International Research Journal of Pharmacy
2012
3
5
403
406
2005
2005
Pharmacopoeia of the People’s Republic of China (2005) English version, Vol. I. People’s Medical Publishing House, Beijing. https://www.amazon.com/Pharmacopoeia-Peoples-Republic-China-v/dp/7117069821
10.1177/1557988306298623
Antimicrobial activities of Tribulus terrestris L. on selected pathogenic microorganisms.
T
Sasikala
author
2014
text
International Journal of Pharmaceutical and Phytopharmacological Research
2014
4
3
182
186
10.15377/2409-983X.2015.02.01.2
10.5504/BBEQ.2011.0036
2008
2008
The Ayurvedic Pharmacopoeia of India (2008) Part – I. Volume-VI. First Ed. Government Of India Ministry Of Health & Family Welfare Department Of Ayush New Delhi, 492 pp. https://naturalingredient.org/wp/wp-content/uploads/API-Vol-6.pdf
10.3897/pharmacia.67.e37433
https://pharmacia.pensoft.net/article/37433/
https://pharmacia.pensoft.net/article/37433/download/pdf/
https://pharmacia.pensoft.net/article/37433/download/xml/
This study describes the identification and quantitative determination of furostanol glycosides in caltrop herb harvested in the fructification period. Furostanol glycosides were identified by thin-layer chromatography (TLC) and quantified by UV-vis spectrophotometry. The furostanol glycosides were visualized on the TLC plate as pink spots after treatment with dimethylamine benzaldehyde solution. UV-vis spectrophotometry quantified these substances to the amount of at least 0.4 %. The data on the identification and quantification of furostanol glycosides obtained in the course of this research was implemented in the development of the Ukrainian State Pharmacopoeia for caltrop herb.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
caltrop
herb
TLC
UV-vis spectrophotometry
Identification and quantitative analysis of furostanol glycosides in caltrop
Research Article
10.3897/pharmacia.67.e54473
2020-10-02
pharmacia
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Chaplynska, Nataliia
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Rudnyk, Viktoriya
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Skrypnyk, Liubov
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Matkovska, Nataliia
2020-10-02
2020-10-02
2020
Pharmacia
2603-557X
0428-0296
67
193-197
2020
10.1007/978-1-4614-8927-6_3
Sarcoidosis of the respiratory organs.
MI
Dzeman
author
2012
text
Praktykuyuchylikar
2012
4
23
28
Sarcoidosis of the respiratory organs: epidemiology, patient structure, treatment results.
VK
Havrysiuk
author
2014
text
Ukrainian therapeutic journal
2014
2
95
100
VK
Havrysiuk
author
2018
Treatment of patients with pulmonary sarcoidosis: methodical guidance.
2018
50 pp
MA
Judson
author
2014
Pulmonarysarcoidosis: A Guide for the practicing clinician.
2014
22 pp
2014
2014
Order of the Ministry of Health of Ukraine (2014) Order of the Ministry of Health of Ukraine № 634, dated 08.09.2014 “Unified clinical protocol of primary, secondary (specialized), tertiary (highly specialized) medical care “Sarcoidosis”.
10.1007/978-1-4614-8927-6_4
Respiratory organs sarcoidosis in clinical practice.
DV
Sergienko
author
2014
text
Rational pharmacotherapy
2014
1
30
37
42
10.1007/978-1-4614-8927-6_2
AA
Vizel
author
2009
2009
2012
2012
X-ray picture of bones and joints in various general infectious, fungal and parasitic diseases (2012) X-ray picture of bones and joints in various general infectious, fungal and parasitic diseases. https://auno.kz/rentgenodiagnostika-zabolevanij-kostej/380-rentgenologicheskaya-kartina-kostej-i-sustavov.html
10.3897/pharmacia.67.e54473
https://pharmacia.pensoft.net/article/54473/
https://pharmacia.pensoft.net/article/54473/download/pdf/
https://pharmacia.pensoft.net/article/54473/download/xml/
Sarcoidosis is a multisystem disease of unknown etiology, at the basis of which there is an inflammatory granulation epithelioid process without a pronounced exudative reaction and without caseous necrosis. This disease has various manifestations. The aim of the study was to investigate the clinical case of generalized sarcoidosis with bone lesion in a patient M., born in 1987. There were performed X-ray examinations, computed tomography (CT), fibrobronchoscopy, determination of calcium levels, angiotensin converting enzyme (ACE), soluble IL-2 receptor in the blood. Dynamic observation was performed for 10 months. The patient started methylprednisolone therapy at a dose of 20 mg/day. Significant positive effect of this treatment was noted. Thus, when patients complain of the bone and joint system problems, examination plan should include radiological examination of thoracic organs, which will allow us to avoid late diagnosis of sarcoidosis or other multisystem diseases that may have similar clinical manifestations.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
clinical case
sarcoidosis
bones of hands
methylprednisolone
Clinical observation of generalized sarcoidosis with damage of bones
Research Article
10.3897/pharmacia.67.e55437
2020-10-02
pharmacia
I Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Ivankiv, Yana
https://orcid.org/0000-0002-2717-9843
I Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Oleshchuk, Oleksandra M.
2020-10-02
2020-10-02
2020
Pharmacia
2603-557X
0428-0296
67
223-228
2020
10.2337/dc18-S008
10.2174/1871527313666140711091400
10.1016/j.jtbi.2010.08.033
10.1007/BF03345555
10.1530/eje.0.1450799
10.3389/fendo.2017.00343
10.3390/ijms14048638
10.3389/fendo.2019.00451
10.1038/nri2925
10.1046/j.1365-2362.32.s3.4.x
10.1016/j.jphotobiol.2009.11.014
10.1186/s13287-018-1114-8
10.1111/j.1600-079X.2012.01014.x
10.1016/j.jchromb.2009.06.001
10.1007/s12551-017-0256-8
10.3390/medicina55060304
10.18632/oncotarget.7863
10.1016/j.pneurobio.2008.04.001
10.1016/j.ygcen.2017.05.013
10.1111/j.1600-079X.2004.00135.x
10.1080/09291016.2019.1566993
10.3389/fendo.2018.00032
10.1186/1742-4933-2-17
10.1159/000148191
10.3390/molecules201018886
10.3389/fendo.2019.00056
10.1038/cmi.2017.7
10.3897/pharmacia.67.e55437
https://pharmacia.pensoft.net/article/55437/
https://pharmacia.pensoft.net/article/55437/download/pdf/
https://pharmacia.pensoft.net/article/55437/download/xml/
Aim: To investigate the effect of melatonin on the immunomodulatory response in experimental type 1 and 2 diabetes mellitus. Methods: Experiments were performed on male rats (180–200 g), purchased from the Experimental Animal Holding,. Animals were maintained in standard diet conditions. Two pathological states were simulated on male rats: experimental type 1 and type 2 diabetes. Melatonin was introduced from 14 to 23 days of experiment intraperitoneally. Levels of immunoglobulin classes A, M and G (Ig A, M, G), circulating immune complexes (CIC), interleukin 1β (EE), interleukin 6 (IL-6), and tumor necrosis factor (TNF-a) were measured. Results: We demonstrated that melatonin in case of immune hyperactivity, can, provide a suppressive effect and is able to enhance immune reactivity under conditions of its limitation, indicating the immunostimulating activity. Furthermore, we found that administration of melatonin decreased inflammatory responses by mediating the levels of immunomodulatory factors, including TNF-α, IL-1β and IL-6. Conclusion: Melatonin is a positive regulator of immune system, may be a potential therapeutic agent, it has no reported side effects.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
experimental diabetes
melatonin
immune system
Immunomodulatory effect of melatonin supplementation in experimental diabetes
Research Article
10.3897/pharmacia.67.e48634
2020-10-23
pharmacia
Medical University of Sofia, Sofia, Bulgaria
author
Shkondrov, Aleksandar
https://orcid.org/0000-0001-5091-6058
Medical University of Sofia, Sofia, Bulgaria
author
Krasteva, Ilina
https://orcid.org/0000-0001-8559-0369
Medical University of Sofia, Sofia, Bulgaria
author
Kozuharova, Ekaterina
https://orcid.org/0000-0001-6795-9660
Medical University of Sofia, Sofia, Bulgaria
author
Zdraveva, Petranka
University of Forestry, Sofia, Bulgaria
author
Savev, Slavcho
2020-10-23
2020-10-23
2020
Pharmacia
2603-557X
0428-0296
67
229-232
2020
10.3897/pharmacia.67.e48634
https://pharmacia.pensoft.net/article/48634/
https://pharmacia.pensoft.net/article/48634/download/pdf/
https://pharmacia.pensoft.net/article/48634/download/xml/
Mauritianin is a relatively rare flavonoid, but several studies revealed its pharmacological potential. In this study overground parts of ten Astragalus species were investigated for mauritianin content to find a reliable source of the compound. The quantity of the flavonoid in each extract was determined by a novel high performance liquid chromatography-high resolution mass spectrometry method. For the first time mauritianin is reported in A. cicer, A. onobrychis, A. glycyphyllos, A. glycyphylloides, A. corniculatus and A. ponticus. Only in A. depressus the compound was not found. Significant differences in mauritianin content (from 4 to 1642 ng/mg dry weight) of the samples were observed. Noteworthy, A. onobrychis var. chlorocarpus and A. cicer could be considered as a potential source of the compound, substituting the rare A. monspessulanus subsp. monspessulanus.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Astragalus
mauritianin
quantitative analysis
flavonoids
UHPLC-MS
Bulgarian species of genus Astragalus as potential sources of mauritianin
Research Article
10.3897/pharmacia.67.e51304
2020-10-23
pharmacia
Bulgarian Drug Agency, Sofia, Bulgaria
author
Zhelyazkova, Margarita
https://orcid.org/0000-0002-6432-0965
Bulgarian Drug Agency, Sofia, Bulgaria
author
Kirilov, Bogdan
Medical University of Sofia, Sofia, Bulgaria
author
Momekov, Georgi
https://orcid.org/0000-0003-2841-7089
2020-10-23
2020-10-23
2020
Pharmacia
2603-557X
0428-0296
67
239-252
2020
10.3897/pharmacia.67.e51304
https://pharmacia.pensoft.net/article/51304/
https://pharmacia.pensoft.net/article/51304/download/pdf/
https://pharmacia.pensoft.net/article/51304/download/xml/
Chemotherapy is one of the therapeutic approaches for cancer treatment and has demonstrated great success with the introduction of selectively acting molecules against specific biomarkers of some types of tumors. Despite this success there is a large unmet need for novel therapies that provide effective control on the progression of advanced or drug-resistant cancer diseases. In this review, we briefly summarized our knowledge of cannabinoids and the endocannabinoid system, as possible agents for cancer therapy. We analyzed the anticancer properties and mechanism of action of cannabidiol (CBD), the main non-psychoactive cannabinoid received from hemp of Cannabis plant. Despite of data for pleiotropic effects of CBD, we here present the results for the efficacy of CBD in the modulation of different stages of cancer development. The analysis of the anticancer properties of CBD is made in relation to the proposed or newly discovered molecular targets of action. Thereafter, we consider the specific effects of CBD on primary tumors, their invasiveness and metastases, whether the influence on identified tumor markers in different types of tumors reflect the therapeutic potential of CBD. The studies reviewed herein indicate that CBD elicit activity through the cannabinoid receptor dependent and independent pathways. The processes such as ceramide production, ER-stress, autophagy and apoptosis, angiogenesis and matrix remodeling also appear to regulate the anticancer activity of CBD. So, the pharmacological basis for therapeutic application of CBD is constructed on the scientific data for its antitumor activity, extensively provided studies in vitro and in vivo in animal tumor models, and available data on the safety profile of clinically approved CBD products. We also try to reduce the deficits of our understanding in relation of pharmacological synergistic interactions of CBD with cytostatic drugs, where data remains limited. It is recognized that more studies for defining the specific molecular and signaling mechanisms of anticancer action of cannabinoids, particularly CBD, requires further evaluation. We believe that the therapeutic advantages of CBD are associated not only with its non-psychoactive behavior, but also are related to its influence on the important biochemical pathways and signal molecules, defining the genome instability and specific changes of the malignant tumor cells.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
anticancer effect
cannabidiol
mechanism of action
pharmacological interactions
safety profile
The pharmacological basis for application of cannabidiol in cancer chemotherapy
Review Article
10.3897/pharmacia.67.e58024
2020-10-23
pharmacia
Clinic of Internal Diseases UMHATEM “N. I. Pirogov”, Sofia, Bulgaria
author
Kobakova, Yozlem Ali
Clinic of Internal Diseases UMHATEM “N. I. Pirogov”, Sofia, Bulgaria
author
Kobakova, Yozlem Ali
Clinic of Internal Diseases UMHATEM “N. I. Pirogov”, Sofia, Bulgaria
author
Atanasov, Petar
Medical University of Pleven, Sofia, Bulgaria
author
Ivanova, Stefka
Medical University of Sofia, Sofia, Bulgaria
Medical University of Pleven, Sofia, Bulgaria
author
Obreshkova, Danka
2020-10-23
2020-10-23
2020
Pharmacia
2603-557X
0428-0296
67
233-237
2020
10.3897/pharmacia.67.e58024
https://pharmacia.pensoft.net/article/58024/
https://pharmacia.pensoft.net/article/58024/download/pdf/
https://pharmacia.pensoft.net/article/58024/download/xml/
The aim of current study was the investigation of complex treatment including nutritional supplement with recognized antioxidant and immunomodulatory properties (malic, glycyrrhizic, ascorbic and folic acids, glucosamine, arginine, glycine, calcium pantothenate, cyanocobalamine, zinc sulfate, pyridoxal) and purely plant systemic protease supplement (Serrapeptase, Bromelaim, Boswellia, Uncaria tomentosa, Quercetin) as a strategy against Covid 19. A clinical case of a 33-year-old patient treated in Internal Clinic Diseases ”Pirogov” against atypical bilaterally viral pneumonia with RT-PCR proven coronavirus have been presented. A complex treatment strategy was applied: Doxycycline, Amikacin sulfate, food additive containing malic and glycyrhizic acids, aminoacids, B-vitamins, antioxidants, proteolytic agent, Methylprednisolone, anticoagulant: Nadroparin calcium, hepatoprotector: Ademethionine. After 16 days hospitalization and two negative RT-PCR tests the patient was discharged in improved condition and home treatment with Levofloxacin hemihydrate, vitamins, probiotics, immunomodulatory and proteolytic products for one month was recommended. Immunostimulating, proteolytic and other products have an important role in complex treatment.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
immunomodulators
proteolytics
pneumonia
SARS-CoV-2
treatment
The role of immunostimulants in the complex therapy of Covid 19 – a clinical case
Review Article
10.3897/pharmacia.67.e38078
2020-10-23
pharmacia
National University of Pharmacy, Kharkiv, Ukraine
author
Shebeko, Sergii
https://orcid.org/0000-0001-9350-7588
National University of Pharmacy, Kharkiv, Ukraine
author
Zupanets, Igor
National University of Pharmacy, Kharkiv, Ukraine
author
Otrishko, Inna
2020-10-23
2020-10-23
2020
Pharmacia
2603-557X
0428-0296
67
253-259
2020
10.3897/pharmacia.67.e38078
https://pharmacia.pensoft.net/article/38078/
https://pharmacia.pensoft.net/article/38078/download/pdf/
https://pharmacia.pensoft.net/article/38078/download/xml/
The efficacy of N-acetylglucosamine in rats with chronic kidney disease is described in this article. The results of the study show that N-acetylglucosamine significantly increased (p<0.05) the excretion of nitrogen compounds (creatinine by 100.4% and urea by 46.6%) and as a result de-creased the level of azotemia. The intensity of free radical oxidation was significantly decreased (p<0.05) (blood conjugated dienes by 32.9%, blood thiobarbituric acid reactive substances – by 14.1%, kidney conjugated dienes – by 40.1% and kidney thiobarbituric acid reactive substances – by 26.3%) and the balance of kidney antioxidant system was restored. N-acetylglucosamine was significantly superior (p<0.05) to comparator quercetin in renal excretory function and nitro-gen metabolism by the most of indicators and was not inferior to the influence on the free radical oxidation and kidney oxidative stress. Thus, N-acetylglucosamine is advisable to further experimental studies at i.m. administration as a chronic kidney disease treatment.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
chronic kidney disease
intramuscular administration
N-acetylglucosamine
rats
Efficacy of the N-acetylglucosamine in experimental therapy of chronic kidney disease
Research Article
10.3897/pharmacia.67.e57134
2020-10-26
pharmacia
Medical University of Sofia, Sofia, Bulgaria
author
Koleva, Kalina
Bulgarian Drug Agency, Sofia, Bulgaria
author
Todorova, Lyubina
Medical University of Sofia, Sofia, Bulgaria
author
Marchev, Stanislav
Medical University of Sofia, Sofia, Bulgaria
author
Vlaskovska, Mila
Medical University of Sofia, Sofia, Bulgaria
author
Nikolov, Rumen
2020-10-26
2020-10-26
2020
Pharmacia
2603-557X
0428-0296
67
283-288
2020
10.3897/pharmacia.67.e57134
https://pharmacia.pensoft.net/article/57134/
https://pharmacia.pensoft.net/article/57134/download/pdf/
https://pharmacia.pensoft.net/article/57134/download/xml/
Pharmacovigilance activities are of vital importance for ensuring effective and safe medicinal products. In order to clarify to which extent marketing authorisation holders (MAHs) meet the requirements of the Law and the Directives related to this activities, we conducted a systematic search among the procedures submitted to the Bulgarian Drug Agency (BDA) related to the implementation of the decisions of the Pharmacovigilance Committee and the Co-ordination Group for Mutual Recognition and Decentralised Procedures – Human (CMDh) for a period of 6 years. The results of the study showed significant discrepancies between regulatory requirements and the behavior of the MAH at the national level. This could be a serious problem, as inadequate or late implementations of the PRAC (Pharmacovigilance Risk Assessment Committee)/CMDh/EC recommendations can lead to untimely informing of healthcare professionals and patients about potential safety concerns and risks related to the use of medicinal products.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
NSAIDs
opioids
regulatory requirements discrepancies
Pharmacovigilance: procedures for uniform assessment of periodic safety update reports and their implementation in national settings. Focus on analgesics
Research Article
10.3897/pharmacia.67.e56880
2020-10-26
pharmacia
Uzhhorod National University, Uzhhorod, Ukraine
author
Nykyforuk, Andriy
https://orcid.org/0000-0001-7590-4539
Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Fira, Liudmyla
Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Lykhatskyі, Petro
Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Pyda, Victor
2020-10-26
2020-10-26
2020
Pharmacia
2603-557X
0428-0296
67
277-282
2020
10.3897/pharmacia.67.e56880
https://pharmacia.pensoft.net/article/56880/
https://pharmacia.pensoft.net/article/56880/download/pdf/
https://pharmacia.pensoft.net/article/56880/download/xml/
Disorders of protein metabolism – one of the pathologies, the treatment of which is an urgent problem of modern medicine and pharmacy. The importance of the problem is stipulated due to the prevalence of diseases that are accompanied by a violation of protein metabolism in the body. Restoration of protein resources during such conditions requires adequate intake of structural components from the outside – proteins, amino acids and other biologically active substances-correctors of protein metabolism. In recent decades, much attention has been paid to the discoveries of anabolic agents of natural origin, especially from plants. We conducted research on the study of the anabolic and antioxidant properties of dry extract of spinach garden leaves on the model of food deprivation in rats. Established, that the dry extract at the dose of 100 mg/kg body weight of animals prevents the development of organic disorders of protein metabolism, oxidative stress, prevents a sharp decrease in body weight and slows down the generalized catabolism caused by starvation in rats. Under the influence of the extract on the model of food deprivation, the diuretic function of the kidneys is preserved at the physiological level.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
spinach garden leaves
dry extract
food deprivation
protein metabolism
spontaneous diuresis
oxidative stress
rats
Experimental research of garden spinach extract as a potential anabolic medicinal product
Research Article
10.3897/pharmacia.67.e57794
2020-10-26
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Nastyukha, Yuliya
https://orcid.org/0000-0001-5281-7168
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Kostyana, Kateryna
https://orcid.org/0000-0003-4688-721X
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Maksymovych, Maria
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Boretska, Olga
2020-10-26
2020-10-26
2020
Pharmacia
2603-557X
0428-0296
67
261-268
2020
10.3897/pharmacia.67.e57794
https://pharmacia.pensoft.net/article/57794/
https://pharmacia.pensoft.net/article/57794/download/pdf/
https://pharmacia.pensoft.net/article/57794/download/xml/
Applying the Classification for Drug-Related Problems (DRPs) of the Pharmaceutical Care Network Europe (V 9.00, 2019) allowed to systematize the information on the use of drugs in elderly patients given in the Annex of the State Drug Formulary of Ukraine. As a result of this work, special warnings and recommendations of the State Drug Formulary were presented together with the possible causes for potential DRPs, which they allow to prevent. The lists of potentially inappropriate medications (PIMs) for the elderly (n = 98), drugs the dosage of which in patients of this age group should be adjusted (n = 127), and drugs that need monitoring (n = 108) were formed. The obtained results can serve as a basis for the development of a specialized geriatric tool to ensure rational pharmacotherapy, in particular in the provision of pharmaceutical care.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
elderly
potentially inappropriate medication
pharmaceutical care
State Drug Formulary
The role of the State Drug Formulary of Ukraine in providing rational pharmacotherapy for elderly patients
Research Article
10.3897/pharmacia.67.e55159
2020-10-26
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Zaliska, Olha
https://orcid.org/0000-0003-1845-7909
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Stasiv, Khrystyna
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Maksymovych, Nataliia
https://orcid.org/0000-0003-0515-0385
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Hrynkiv, Yaryna
https://orcid.org/0000-0002-6977-6136
2020-10-26
2020-10-26
2020
Pharmacia
2603-557X
0428-0296
67
269-276
2020
10.3897/pharmacia.67.e55159
https://pharmacia.pensoft.net/article/55159/
https://pharmacia.pensoft.net/article/55159/download/pdf/
https://pharmacia.pensoft.net/article/55159/download/xml/
The prevalence of infertility has risen 2.9 times in recent years in Ukraine. The use of assisted reproductive technologies (ART) is needed. The State program for infertility treatment by budget funds was approved in Ukraine since 2004. However, funding for this program is enough only for 500–600 cycles per year, but ART centers conducted more than 27,000 cycles in 2018. This means that many women have to pay out-of-pockets. Our research has shown that between 2000 and 2018, the number of ART clinics and the number of cycles, pregnancies, and children born increased significantly in Ukraine. The rate of ART cycles per 1 million population in Ukraine has increased from 226.9 to 655.3, but it is lower than the WHO recommended rate 800–1000 cycles per 1 million population. Changes in the structure of ART cycles for 2000–2018, the increase of ICSI, and egg donation cycles are shown. It requires an adequate supply by hormonal drugs for ovarian stimulation in ART centers. Since 2004 Ministry of Health of Ukraine annually approved the list of medicines for State program of ART. Only 8 drugs were purchased by the state funds, which amounted for USD 227 438 in 2017, 2018. A retrospective analysis of drug prescriptions in short and long stimulation protocols was performed. The frequency of drug prescriptions according to the ATC-classification based on medical records was determined, all prescriptions were accordance with the requirements of ART treatment standards in Ukraine. The costs of the three hormonal stimulation schemes, which were the most prescribing in ART center, were calculated. It was found that costs for hormonal schemes increased by 22–54% during 2015–2020, it significantly reduces the availability and affordability of ART for the population in Ukraine.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
infertility
assisted reproductive technologies
cost
ovarian stimulation
affordability
The trends of assisted reproductive technologies and cost for ovarian stimulation protocols in Ukraine
Research Article
10.3897/pharmacia.67.e54777
2020-11-03
pharmacia
National University of Pharmacy, Kharkov, Ukraine
author
Kotvitska, Alla
National University of Pharmacy, Kharkov, Ukraine
author
Nemchenko, Alla
National University of Pharmacy, Kharkov, Ukraine
author
Nazarkina, Viktoria
https://orcid.org/0000-0002-0767-6180
2020-11-03
2020-11-03
2020
Pharmacia
2603-557X
0428-0296
67
295-301
2020
10.3897/pharmacia.67.e54777
https://pharmacia.pensoft.net/article/54777/
https://pharmacia.pensoft.net/article/54777/download/pdf/
https://pharmacia.pensoft.net/article/54777/download/xml/
Based on generalization of the modern international experience the areas of development of the methodology for training specialists in Health Technology Assessment (НТА) system have been determined; they reflect the categories of information consumers, the goal, methods, organizers and intermediaries of knowledge transfer. The analysis of the market of educational services, educational curricula and Internet resources in the field of НТА has been conducted. Based on the results they are classified into 4 groups – academic programs, professional improvement and continuing education, short-term training and information for various target groups, online courses at the pre-graduate level and for postgraduate training. According to the all-Ukrainian survey the necessity for training specialists in the field of “expert in assessing health technologies” has been confirmed. Based on the information needs, requirements of stakeholders and international experts in НТА a qualification characteristic, and on its basis – the professional academic program, have been developed at the National University of Pharmacy, as well as training of the corresponding specialists has been started. In the future, it is planned to expand educational services and conduct short-term courses and workshops, as well as online training of specialists in НТА at the postgraduate level.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
educational program
health technology assessment
training of specialists
The relevance of training specialists in the Health Technology Assessment in the world and Ukraine
Research Article
10.3897/pharmacia.67.e57752
2020-11-03
pharmacia
Medical University of Pleven, Pleven, Bulgaria
author
Lebanova, Hristina
https://orcid.org/0000-0003-4835-6965
Bulgarian Pharmaceutical Union, Sofia, Bulgaria
author
Balkanski, Stefan
https://orcid.org/0000-0002-3193-7918
Medical University of Sofia, Sofia, Bulgaria
author
Naseva, Emilia
https://orcid.org/0000-0002-1282-8441
Medical University of Sofia, Sofia, Bulgaria
author
Getov, Ilko N.
2020-11-03
2020-11-03
2020
Pharmacia
2603-557X
0428-0296
67
289-293
2020
2019
2019
AESGP (2019) Evolving the self-care environment. https://aesgp.eu/content/uploads/2020/05/AESGP_ActivityReport2019-interactive.pdf [August 19, 2020]
10.1186/s13104-015-1737-0
10.1007/s00228-006-0107-1
10.1136/thorax.58.10.851
Patients’ knowledge about the “Rx-to-OTC” switch process in Bulgaria.
V
Belcheva
author
2016
text
Scripta Scientifica Pharmaceutica
2016
3
39
39
Study of Bulgarian patient’s awareness on the process of medicines reclassification in terms of drug safety and effectiveness /part I/.
V
Belcheva
author
2017
text
Health Policy and Management
2017
17
18
20
C
Bonsignore
author
2014
2014
10.1345/aph.1L605
10.1016/j.cmi.2018.09.022
2013
2013
EPPOSI (2013) Consumer Perceptions of Self Care in Europe Quantitative Study 2013 2 EPPOSI self care barometer: quantitative study perceptions of self care in Europe.
S
Georgiev
author
2018
2018
10.3390/pharmacy7030106
10.2165/00002018-200124140-00002
M
Irfadh
author
2013
2013
10.1159/000092989
10.1001/jama.287.3.337
10.1023/A:1008733207854
Practical tool to assess reliability of web-based medicines information.
H
Lebanova
author
2014
text
Medicinski Glasnik
2014
11
221
227
10.1007/s40264-018-0719-2
10.1080/13696998.2017.1390473
10.2174/157488609788172982
10.12955/cbup.v4.847
10.1016/j.sapharm.2008.04.002
The role of pharmacist as an advisor on responsible self-medication: a survey.
A
Tsvetkova
author
2014
text
World J of Pharmacy and Pharmaceutical Sciences
2014
3
325
335
2000
2000
WHO (2000) Guidelines for the Regulatory Assessment of Medicinal Products for use in Self-Medication. https://apps.who.int/iris/bitstream/handle/10665/66154/WHO_EDM_QSM_00.1_eng.pdf [August 18, 2020]
10.1186/1472-6904-11-19
2019
2019
ZLPHM (2019) Medicinal Products in Human Medicine Act.
10.3897/pharmacia.67.e57752
https://pharmacia.pensoft.net/article/57752/
https://pharmacia.pensoft.net/article/57752/download/pdf/
https://pharmacia.pensoft.net/article/57752/download/xml/
The aim of the study is to identify the current practices of self-medication in Bulgaria, estimate the extent patients are influenced by information about the safety of OTC products and pharmacists’ role and behavior in the process. An anonymous, questionnaire-based, descriptive study was carried out among 280 pharmacists. The majority of the patients (92.6%) ask for pharmacists’ advice before buying OTC products. Most often patients need OTC drugs for mild conditions (80.7%), to save time (63.9%), and because they are unable to consult a physician (61.9%). The medium duration of consultation for an OTC product is between 5 and 10 minutes. The main barriers hindering the process are unwillingness from the patient (72.5%), lack of time (27.5%), and lack of confidentiality (26.6%). OTC medicines are considered safer than prescription-only medicines by pharmacists. Pharmacists could substantially contribute to rational self-medication and alleviate the burden on healthcare systems.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
self-medication
pharmaceutical counseling
over-the-counter medicines
What does self-medication counseling in Bulgarian community pharmacies look like – a field study
Research Article
10.3897/pharmacia.67.e47298
2020-11-06
pharmacia
Medical University of Sofia, Sofia, Bulgaria
author
Andreeva-Gateva, Pavlina
https://orcid.org/0000-0002-3791-8654
Medical University of Sofia, Sofia, Bulgaria
author
Bakalov, Dimitar
https://orcid.org/0000-0002-1049-8813
Medical University of Sofia, Sofia, Bulgaria
author
Sabit, Zafer
Medical University of Sofia, Sofia, Bulgaria
author
Tafradjiiska-Hadjiolova, Radka
2020-11-06
2020-11-06
2020
Pharmacia
2603-557X
0428-0296
67
311-315
2020
10.3897/pharmacia.67.e47298
https://pharmacia.pensoft.net/article/47298/
https://pharmacia.pensoft.net/article/47298/download/pdf/
https://pharmacia.pensoft.net/article/47298/download/xml/
Aryl hydrocarbon receptors (AhR) are regulators of the expression of cytochrome P-450 isoforms, mediating a wide variety of the effects of substances from the endogenous or exogenous origin, including those produced from the microbiome. An exciting new aspect of their activity is their localization in the brain and their potential to modulate the action of the immune system. AhR is emerging as an essential toxicological and therapeutic target for neuromodulation. Further studies are needed for elucidating their utility as drug-targets.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
dioxin
indoles
toxicology
neuroprotection
Aryl hydrocarbon receptors as potential therapeutic targets
Review Article
10.3897/pharmacia.67.e56511
2020-11-06
pharmacia
Can Tho University of Medicine and Pharmacy, Can Tho, Vietnam
Peoples’ Friendship University of Russia, Moscow, Russia
author
Tran, Van De
https://orcid.org/0000-0003-0421-5079
Peoples’ Friendship University of Russia, Moscow, Russia
author
Dorofeeva, Valeria V.
Peoples’ Friendship University of Russia, Moscow, Russia
author
Loskutova, Ekaterina E.
Peoples’ Friendship University of Russia, Moscow, Russia
author
Lagutkina, Tatyana P.
Peoples’ Friendship University of Russia, Moscow, Russia
author
Kosova, Irina V.
Peoples’ Friendship University of Russia, Moscow, Russia
author
Gribkova, Elena I.
Peoples’ Friendship University of Russia, Moscow, Russia
author
Galkina, Galina A.
Peoples’ Friendship University of Russia, Moscow, Russia
author
Pak, Tatiana V.
Peoples’ Friendship University of Russia, Moscow, Russia
author
Ibragimova, Alfia N.
Can Tho University of Medicine and Pharmacy, Can Tho, Vietnam
author
Le, Minh Hoang
Can Tho University of Medicine and Pharmacy, Can Tho, Vietnam
author
Cao, Thi Tai Nguyen
2020-11-06
2020-11-06
2020
Pharmacia
2603-557X
0428-0296
67
303-309
2020
10.3897/pharmacia.67.e56511
https://pharmacia.pensoft.net/article/56511/
https://pharmacia.pensoft.net/article/56511/download/pdf/
https://pharmacia.pensoft.net/article/56511/download/xml/
Introduction: The purpose of this study was to understand elderly consumers’ satisfaction on the community pharmacy services in Ho Chi Minh City, Vietnam. Materials and methods: A sample of 32 consumers, aged over 60, was recruited in four pharmacies in Ho Chi Minh City, Vietnam, from December, 2017 to January, 2018. Q-methodology was used to identify the patterns of consumers’ satisfaction. Results: Two distinct patterns of elderly consumers’ viewpoints toward the community pharmacy services were identified. Elderly consumers in viewpoint 1 were satisfied with the geographic accessibility of community pharmacies. Elderly consumers in viewpoint 2 were satisfied with the affordability of medicines. However, consumers of both viewpoints expressed dissatisfaction with the pharmacy staff counseling on missed doses, storage, side effects, and interactions of medicines. Conclusion: The results from this study suggest that pharmacy staff need to spend more time for consultations on proper medication use with elderly consumers.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
community pharmacy
elderly consumer
Q-methodology
satisfaction
Vietnam
Elderly consumers’ satisfaction with the quality of community pharmacy services in Ho Chi Minh City, Vietnam: a Q-methodology study
Research Article
10.3897/pharmacia.67.e56112
2020-11-26
pharmacia
Medical University of Sofia, Sofia, Bulgaria
author
Voynova, Maria
Medical University of Sofia, Sofia, Bulgaria
author
Shkondrov, Aleksandar
https://orcid.org/0000-0001-5091-6058
Medical University of Sofia, Sofia, Bulgaria
author
Kondeva-Burdina, Magdalena
Medical University of Sofia, Sofia, Bulgaria
author
Krasteva, Ilina
2020-11-26
2020-11-26
2020
Pharmacia
2603-557X
0428-0296
67
317-323
2020
Morphology and subdivision of Amanita and a monograph on its section Lepidella. Persoonia.
C
Bas
author
1969
text
Rijksherbarium, Leiden
1969
5
4
285
579
10.3109/15563659208994442
10.1002/(SICI)1521-3773(19990315)38:6%3C795::AID-ANIE795%3E3.0.CO;2-7
10.1016/0028-3908(77)90063-6
Amanita muscaria: Present understanding of its chemistry.
P
Catalfomo
author
1970
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Bulletin on Narcotics
1970
22
4
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10.1038/npp.2009.203
10.1016/0031-9422(74)80096-8
10.1016/j.neuroscience.2004.01.049
10.1523/JNEUROSCI.21-05-01720.2001
10.1007/BF00411168
10.1007/BF00966163
10.1007/s00572-011-0388-9
Isolation, structure and synthesis of central active compounds from Amanita muscaria (L. ex Fr.) Hooker.
CH
Eugster
author
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Efron
author
1979
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Public Health Service Publication Number
1979
1645
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10.1002/hlca.19670500119
10.1016/S0040-4039(00)90133-3
Iconografia de macromicetos de Mexico. I. Amanita.
E
Perez-Silva
author
1991
text
1991
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10.1080/02791072.2010.10400712
2012
2012
Food and Drug Administration (2012) Bad Bug Book, Foodborne pathogenic microorganisms and natural toxins. Second edn. Mushroom toxins: 218 pp. https://www.fda.gov/food/foodborne-pathogens/bad-bug-book-second-edition
10.1016/S0040-4039(00)90156-4
10.1016/0006-8993(86)90611-6
10.1016/j.ympev.2008.04.029
10.3389/fnsys.2018.00072
10.1016/B978-008045046-9.00868-8
10.1002/rmb2.12061
10.1248/bpb.17.1460
10.1007/BF00402378
10.1016/j.jep.2016.04.009
10.1016/S0074-7742(08)60722-9
10.1007/s00414-011-0599-9
10.3892/ijmm.2016.2597
10.1155/NP.2000.19
10.1016/S0006-8993(97)01324-3
SM
Jerrold
author
2005
2005
10.1017/S0953756203007305
10.1111/j.1471-4159.1985.tb04052.x
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Fate of muscimol in the mouse.
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Ott
author
1975
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Phvsioloyical Chemistry and Physics
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Persoon
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10.1016/j.carbpol.2013.06.061
10.1016/j.toxicon.2005.01.005
10.1002/ana.410040316
10.1038/273392a0
10.1016/0016-5085(88)90288-0
10.1097/FTD.0b013e31828741a5
10.1161/01.STR.20.2.281
10.1515/znc-2007-11-1201
10.1248/yakushi1947.84.12_1186
10.1002/ijc.2910520616
Amanita distribution in the Americas with comparison to eastern and southern Asia and notes on spore character variation with latitude and ecology.
R
Tulloss
author
2005
text
Mycotaxon
2005
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10.1152/ajpgi.00344.2016
P
Waser
author
1979
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10.2478/fobio-2014-0014
10.3897/pharmacia.67.e56112
https://pharmacia.pensoft.net/article/56112/
https://pharmacia.pensoft.net/article/56112/download/pdf/
https://pharmacia.pensoft.net/article/56112/download/xml/
Amanita muscaria, commonly known as fly agaric, is a basidiomycete. Its main psychoactive constituents are ibotenic acid and muscimol, both involved in ‘pantherina-muscaria’ poisoning syndrome. The rising pharmacological and toxicological interest based on lots of contradictive opinions concerning the use of Amanita muscaria extracts’ neuroprotective role against some neurodegenerative diseases such as Parkinson’s and Alzheimer’s, its potent role in the treatment of cerebral ischaemia and other socially significant health conditions gave the basis for this review. Facts about Amanita muscaria’s morphology, chemical content, toxicological and pharmacological characteristics and usage from ancient times to present-day’s opportunities in modern medicine are presented.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Amanita muscaria
muscimol
ibotenic acid
Toxicological and pharmacological profile of Amanita muscaria (L.) Lam. – a new rising opportunity for biomedicine
Review Article
10.3897/pharmacia.67.e35069
2020-11-27
pharmacia
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Cherepyuk, Olena
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Oktysyuk, Yuriy
https://orcid.org/0000-0001-7741-2490
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Bazalytska, Aleksandra
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
author
Rozhko, Mykola
2020-11-27
2020-11-27
2020
Pharmacia
2603-557X
0428-0296
67
347-350
2020
10.14219/jada.archive.2000.0027
Indexes of dental caries in children of Ukraine, Russia and Belorussia over the period 1990–2010.
ОV
Denha
author
2013
text
Innovation of stomatology
2013
2
30
36
Complex assessment of dental health and physical development of children in the regions with polluted environment, Fluoride and Iodine deficiency.
EV
Bezvushko
author
2010
text
The environment and health
2010
1
45
47
EV
Borovskyy
author
2001
Biology of oral cavity.
2001
304 pp
Modern aspects of donosologic diagnosis of dental caries.
VV
Hilyaseva
author
2012
text
Modern problems of science and education
2012
5
47
54
HD
Ovruckyy
author
1991
Immunology of dental caries.
1991
96 pp
AY
Atler
author
2007
Immunologia.
2007
364 pp
Microbial colonization of oral cavity and level of s-IgA inpeople with different intensity of caries.
VV
Chereda
author
2012
text
Ukrainian stomatological almanach
2012
4
19
21
10.1007/s13312-011-0113-y
10.4317/jced.54271
2013
2013
WHO [World Health Organization] (2013) Oral health surveys: basic methods – 5th edition. WHO, 135 pp.
The level of s-IgA in oral liquid of children under 3 years old affected by caries.
LО
Chomenko
author
2012
text
News of stomatology
2012
1
108
109
Microflora of dental plaque in children according to intensity of caries.
NI
Smolyar
author
2013
text
Prophylactic and pediatric stomatology
2013
2
26
29
10.15574/SP.2015.68.132
Comparison of salivary IgA levels in caries free and caries active children.
D
Doifode
author
2011
text
International Journal of Clinical Dental Science
2011
2
1
10
14
10.15171/joddd.2015.022
10.17796/1053-4628-39.4.343
10.3897/pharmacia.67.e35069
https://pharmacia.pensoft.net/article/35069/
https://pharmacia.pensoft.net/article/35069/download/pdf/
https://pharmacia.pensoft.net/article/35069/download/xml/
Dental caries is the most prevalent pathological condition in the child population of Ukraine. Despite the achievements of medical science, dental caries in children remains one of the most actual problems nowadays. Epidemiological investigations conducted in different regions of Ukraine demonstrate that 62% of children have decay and in many parts of the country the statistic is even higher up to 96.5% when the intensity of carious damage fluctuates between 3.2 to 7.2 teeth. This article reflects the results of the study regarding correction of disordered oral immunity indexes by immune modulator “Esberitox” in children affected by different caries activity. We have confirmed the high efficiency of the recommended scheme of caries prophylaxis that is proved by positive dynamic of oral immunological indexes observed 24 month after the beginning of the research. There was a certain rise of lysozyme by about 21.1% and s-IgA has increased by 28% in children from prophylactic groups in comparison with control groups.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
children
dental caries
lysozyme
oral liquid
secretory immunoglobulin A
Correction of disordered oral immunity in children affected by dental caries with herbal immune modulator “Esberitox”
Research Article
10.3897/pharmacia.67.e47528
2020-11-27
pharmacia
Medical University – Sofia, Sofia, Bulgaria
author
Popova, Teodora
Medical University – Sofia, Sofia, Bulgaria
author
Voycheva, Christina
https://orcid.org/0000-0002-6536-4898
Medical University – Sofia, Sofia, Bulgaria
author
Tzankov, Borislav
2020-11-27
2020-11-27
2020
Pharmacia
2603-557X
0428-0296
67
351-356
2020
10.1021/ja062286z
10.1080/10717540490265252
10.1021/cm0485658
10.1016/j.biomaterials.2009.09.060
10.1021/cm0210041
10.1016/j.chroma.2006.04.055
10.1038/359710a0
10.1016/j.cej.2007.07.078
10.1016/j.micromeso.2005.12.004
10.1021/ja038250d
10.1021/ar600032u
10.1021/nl048774r
10.1021/cm0011559
10.1021/cm051198v
10.1016/j.ces.2005.08.021
10.1002/(SICI)1521-3773(19990115)38:1/2%3C56::AID-ANIE56%3E3.0.CO;2-E
10.1126/science.279.5350.548
10.1021/ja974025i
10.3897/pharmacia.67.e47528
https://pharmacia.pensoft.net/article/47528/
https://pharmacia.pensoft.net/article/47528/download/pdf/
https://pharmacia.pensoft.net/article/47528/download/xml/
The present study explored solvent impregnation drug loading process of the poorly soluble non-steroid anti-inflammatory drug indomethacin on MCM-41 type mesoporous silica carrier. Different technological factors that can influence drug-loading process as time of reaction, temperature, use of non-solvent as well as different ratios between drug and MCM-41 were studied. TEM and DLS were used to characterize physicochemical properties of obtained particles. The influence of drug-loading rate on dissolution process were studied using in-vitro release tests. Our results established that changes in explored technological factors could lead to different indomethacin loading. Moreover, the in-vitro release tests proved that drug loading rate had a direct influence on indomethacin release from MCM-41 particles. Our finding suggested that by tuning the main technological factors it would be possible different drug delivery systems with different drug loading rate to be obtained.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
drug loading
indomethacin
MCM-41
technological factors
Study on the influence of technological factors on drug loading of poorly water-soluble drug on MCM-41 mesoporous carrier
Research Article
10.3897/pharmacia.67.e54170
2020-11-27
pharmacia
Ternopil National Medical University, Ternopil, Ukraine
author
Budniak, Liliia
https://orcid.org/0000-0002-4869-1344
Ternopil National Medical University, Ternopil, Ukraine
author
Vasenda, Marjana
Ternopil National Medical University, Ternopil, Ukraine
author
Marchyshyn, Svitlana
Ternopil National Medical University, Ternopil, Ukraine
author
Kurylo, Khrystyna
2020-11-27
2020-11-27
2020
Pharmacia
2603-557X
0428-0296
67
373-378
2020
In vitro antioxidant, antibacterial activity and phytochemical studies of Primula denticulata – an important medicinal plant of Kashmir Himalaya.
K
Aslam
author
2015
text
International Journal of pharmacology research
2015
5
3
49
56
10.7439/ijasr.v1i2.1792
10.14499/indonesianjpharm27iss2pp74
Statistical approaches in analysis of variance: from random arrangements to latin square experimental design.
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Bolboaca
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2009
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10.1016/j.phytochem.2013.11.018
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10.1667/0033-7587(2003)159[0371:EOTEOA]2.0.CO;2
Influence of phytocompositions on dynamics of changes in basal glycemia and glycemia in oral glucose tolerance test in rats with streptozotocin-nicotinamide-induced diabetes mellitus type 2.
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Kurylo
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2020
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Georgian medical news
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10.7717/peerj.2101
Investigation of phenolic compounds about ground organs of cultivated species genus Primula L.
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Marchyshyn
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The Pharma Innovation
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42
Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potential.
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Narayana
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NIST/SEMATECH (2013) NIST/SEMATECH e-Handbook of Statistical Methods [Internet] Engineering Statistics Handbook. [updated 2019 Nov 7; cited 2019 Nov 7]. http://www.itl.nist.gov/div898/handbook/
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10.1038/srep24432
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Popov
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5411
5419
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Rozycki
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2003
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H
Rushing
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2013
Design and Analysis of Experiments by Douglas Montgomery: A Supplement for Using JMP.
2013
277 pp
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Shakya
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2016
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4
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Biological screening of plants extract showing hypoglycaemic and wound healing properties: Capparis zeylanica and Primula denticulata.
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Singh
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2014a
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10.24959/ubphj.18.157
Comparative evaluation of the hepatoprotective, antioxidant and choleretic activity of flavonoid drugs.
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1988
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Vrachebnoe Delo
1988
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10.22159/ijap.2019v11i5.33909
10.31688/ABMU.2018.53.4.06
2015
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The State Pharmacopoeia of Ukraine [in 3 vol.] (2015) State Enterprise “Ukrainian Scientific Expert Pharmacopoeial Center of the Quality of Medicines” (2nd iss.). Kharkiv: State Enterprise “Ukrainian Scientific and Experimental Pharmacopoeial Center for the Quality of Medicinal Products”, 1128 pp.
10.1021/jf0347160
Research of technological factors on the extraction process of bas from walnut membranes.
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Vasenda
author
2018
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10.1177/1934578X0900400310
10.15587/2313-8416.2015.57434
10.3897/pharmacia.67.e54170
https://pharmacia.pensoft.net/article/54170/
https://pharmacia.pensoft.net/article/54170/download/pdf/
https://pharmacia.pensoft.net/article/54170/download/xml/
Herbal medicines are widely used in the complex treatment of various diseases. Therefore, theoretical and practical interest is the in-depth study of drumstick primrose (Primula denticulata Smith). The study aimed to determine the optimal extraction mode of flavonoids and reducing compounds of drumstick primrose leaves. The concentration of ethanol, the ratio of raw materials and extractant, and extraction method were studied by dispersion analysis. This allowed reducing the number of experiments from 64 to 16. To obtain the alcohol extract of drumstick primrose leaves with the highest content of reducing compounds and flavonoids, found that maceration is the optimal method of extraction, the ratio of raw materials to extractant should be 1 to 5 and 40% ethanol is the most appropriate extractant.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Primula denticulata Smith
dispersion analysis
flavonoids
reducing compounds
Determination of the optimum extraction regime of reducing compounds and flavonoids of Primula denticulata Smith leaves by a dispersion analysis
Research Article
10.3897/pharmacia.67.e52262
2020-11-27
pharmacia
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Pankevych, Ostap
https://orcid.org/0000-0002-1702-8689
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Hromovyk, Bohdan
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
author
Horilyk, Artem
Lviv Polytechnic National University, Lviv, Ukraine
author
Bilushchak, Halyna
2020-11-27
2020-11-27
2020
Pharmacia
2603-557X
0428-0296
67
379-382
2020
2020
2020
EAHP [European Association of Hospital Pharmacists] (2020) European Association of Hospital Pharmacists Official website. https://www.eahp.eu/about-us/who-we-are
Developing a Structure of Good Pharmacy Practice Standards.
LO
Hala
author
2014
text
Zaporizhzhya medical journal
2014
1
87
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10.1093/ajhp/45.5.1071
Institutional dimension of international pharmaceutical self-regulation.
BP
Hromovyk
author
2017
text
Pharmaceutical Review
2017
1
41
45
54
Situational analysis of self-regulation of pharmaceutical activity in Ukraine.
BP
Hromovyk
author
2018
text
Science and Innovation
2018
2
5
8
2020
2020
IPF [International Pharmaceutical Federation] (2020) International Pharmaceutical Federation Official website. https://www.fip.org/about
10.1080/14737167.2020.1751612
Prospects for development of the pharmaceutical market of Ukraine in the conditions of intensification of European integration processes of.
LL
Litvinenko
author
2015
text
Problems of Improving the Efficiency of Infrastructure
2015
40
51
56
2020
2020
Marketing-Helping (2020) Sample, its size and structure. http://marketing-helping.com/konspekti-lekcz/25-kurs-marketinga/493-2011-01-19-19-44-00.html [In Ukrainian]
10.12968/bjcn.2003.8.12.11854
VM
Nazarkina
author
2015
2015
Structuring factors influencing the formation of the Institute of Pharmaceutical Self-Regulation in Ukraine.
OB
Pankevych
author
IP
Klimov
author
2017
text
Karlovy Vary, Skleněný Můstek
2017
205
212
OB
Pankevych
author
2019
Strategy for the development of the pharmaceutical self-regulatory institute in Ukraine: scientific and methodological recommendations.
2019
24 pp
2020
2020
PGEU [Pharmaceutical Group of the European Union] (2020) Pharmaceutical Group of the European Union. Vision and Mission. https://www.pgeu.eu/vision-and-mission/
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2020
Practical pharmacy (2020) Practical pharmacy. [Facebook professional society URL:] https://www.facebook.com/groups/303252657278677/permalink/525329025071038/
VYe
Savka
author
2008
2008
Identifying the priority areas for improving of state and public regulation in pharmacy.
ІV
Susharina
author
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text
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2017a
1
38
43
10.32352/0367-3057.1.17.02
The role of public organizations and business entities in the self-regulation of pharmacy practice in Ukraine.
LM
Unhurian
author
2014
text
Vestnik of the South Kazakhstan State Pharmaceutical Academy
2014
2
19
25
10.1016/0144-8188(91)90017-8
10.3897/pharmacia.67.e52262
https://pharmacia.pensoft.net/article/52262/
https://pharmacia.pensoft.net/article/52262/download/pdf/
https://pharmacia.pensoft.net/article/52262/download/xml/
The aim of our study was to investigate the views of pharmacists on understanding the nature and necessity of implementing the self-regulation of pharmaceutical practice in Ukraine. We conducted an anonymous questionnaire survey among pharmaceutical specialists (n = 827) from the majority (16 out of 25) of the regions controlled by the Ukrainian government during the period from October till December 2018. This survey revealed lack of pharmacists’ understanding the concept of self-regulation of pharmaceutical practice. On the one hand, most respondents do not oppose the implementing of self-regulation and don’t reject exclusive membership in self-regulated pharmaceutical organizations for only pharmaceutical specialists, but on the other hand the survey showed a low willingness voluntarily to become a member of such organizations and also low level of knowledge of self-regulation process. This indicates the need to update the self-regulation process through providing training activities for pharmacists, the development of legal framework and the government’s assistance of self-regulation of pharmaceutical practice.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Self-regulation
pharmaceutical activities
pharmaceutical practice
pharmaceutical societies
public organizations
Ukraine
The study of pharmaceutical specialists’ thoughts concerning self-regulation of pharmaceutical activity in Ukraine
Research Article
10.3897/pharmacia.67.e58392
2020-11-27
pharmacia
UCSI Universit, Kuala Lumpur , Malaysia
author
Okechukwu, Patrick
UCSI Universit, Kuala Lumpur , Malaysia
author
Sharma, Mridula
UCSI Universit, Kuala Lumpur , Malaysia
author
Tan, Wei Hui
UCSI Universit, Kuala Lumpur , Malaysia
author
Chan, Hor Kuan
UCSI Universit, Kuala Lumpur , Malaysia
author
Chirara, Kavita
UCSI Universit, Kuala Lumpur , Malaysia
author
Gaurav, Anand
UCSI Universit, Kuala Lumpur , Malaysia
author
Al-Nema, Mayasah
https://orcid.org/0000-0001-8352-5125
2020-11-27
2020-11-27
2020
Pharmacia
2603-557X
0428-0296
67
363-371
2020
10.4103/0975-1483.59320
10.3923/rjmp.2018.48.56
10.1016/j.sajb.2018.01.018
10.1016/j.diabres.2018.02.023
10.3390/ijms19102883
10.4239/wjd.v6.i6.850
10.1155/2018/2164345
10.3390/molecules23081998
10.1210/jcem.97.1.zeg27a
10.1177/2040622319875305
10.1021/jf0489926
10.2174/1381612821666150710145753
M
Pandithurai
author
2015
2015
10.1155/2019/2161085
10.2337/db11-1825
10.1002/cbdv.201800025
10.1088/1757-899X/716/1/012007
10.4103/0253-7613.161270
10.18203/2320-6012.ijrms20170619
10.1016/j.heliyon.2018.e00810
10.1016/j.fshw.2014.11.003
10.3897/pharmacia.67.e58392
https://pharmacia.pensoft.net/article/58392/
https://pharmacia.pensoft.net/article/58392/download/pdf/
https://pharmacia.pensoft.net/article/58392/download/xml/
Palmatine a protoberberine alkaloid has been previously reported to possess in vivo antidiabetic and antioxidant property. The aim of the experiment is to evaluate the in vitro antidiabetic activity and in-silico studies of the binding energies of Palmatine, acarbose, and Sitagliptin with the three enzymes of alpha-amylase, alpha-glucosidase, and dipeptidyl peptidase-IV (DPP-IV). The in vitro antidiabetic study was done by evaluating the inhibitory effect of palmatine on the activities of alpha-amylase, alpha-glucosidase, and DPP-IV. Acarbose, and sitagliptin was used as standard drug. The molecular docking study was performed to study the binding interactions of palmatine with alpha-glucosidase, a-amylase, and DPP-IV. The binding interactions were compared with the standard compounds Sitagliptin and acarbose. Palmatine with IC50 (1.31 ± 0.27 µM) showed significant difference of (< 0.0001) higher inhibiting effect on alpha-amylase and weak inhibiting effect on alpha-glucosidase enzyme with IC50 (9.39 ± 0.27 µM) and DPP-IV with IC50 (8.7 ± 1.82 µM). Palmatine possess inhibition effect on the three enzymes.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Alpha-glucosidase
alpha-amylase
dipeptidyl peptidase-IV
diabetes mellitus
palmatine
In-vitro anti-diabetic activity and in-silico studies of binding energies of palmatine with alpha-amylase, alpha-glucosidase and DPP-IV enzymes
Research Article
10.3897/pharmacia.67.e51334
2020-11-27
pharmacia
Medical University of Sofia, Sofia, Bulgaria
author
Kozuharova, Ekaterina
https://orcid.org/0000-0001-6795-9660
Medical University of Sofia, Sofia, Bulgaria
author
Benbassat, Niko
https://orcid.org/0000-0001-8876-2728
Medical University of Sofia, Sofia, Bulgaria
author
Ionkova, Iliana
2020-11-27
2020-11-27
2020
Pharmacia
2603-557X
0428-0296
67
357-362
2020
10.1021/jf0210728
10.1021/bk-1989-0387.ch002
Insecticidal properties of the indigobush (Amorpha fruticosa).
CH
Brett
author
1946
text
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2019
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Cao
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2004
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Csiszár
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Acta Silvatica et Lignaria Hungarica
2009
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10.1073/pnas.95.7.3380
10.1093/ee/8.6.1123
10.1073/pnas.0803601105
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10.1016/S0014-827X(01)01092-8
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EV
Georgiev
author
2000
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2000
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10.1016/B978-012370467-2/50139-5
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Hovanet
author
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10670
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Ivanescu
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Kariuki
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Zahariev
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10.3897/pharmacia.67.e51334
https://pharmacia.pensoft.net/article/51334/
https://pharmacia.pensoft.net/article/51334/download/pdf/
https://pharmacia.pensoft.net/article/51334/download/xml/
The high tolerance of various habitat conditions and potent propagation ability of Amorpha fruticosa L. (Fabaceae) promote its aggressive invasive behaviour. The aim of this study is to evaluate 1) several populations of this plant by the potential yield of the fruit, 2) the approximate yield of the essential oils, and 3) composition of the essential oil. The potential yield of fruit is evaluated based on extrapolations of weight and number of fruits per infrutescence, number of infrutescences per plant and number of individuals per population. Steam extraction of the essential oil was performed and GS/MS analysis of the composition of the essential oil. The populations on our key plots are big enough for harvesting with fruit/infrutescence 152 ±15 (n = 20) and infrutescences/shrub 436 ±157 (n = 20). The yield is 0.83 ml/100g. We identified 22 components with major constituents caryophyllene (17.64%) α-guaiene (14.70%), naphthalene (6.75%), γ-muurolene + (5.98%).
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Amorpha fruticosa
chemical composition
essential oil
yield
The invasive alien species Amorpha fruticosa in Bulgaria and its potential as economically prospective source of valuable essential oil
Research Article
10.3897/pharmacia.67.e57242
2020-11-27
pharmacia
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Dedic, Mirza
https://orcid.org/0000-0002-4951-7516
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Gutic, Sanjin
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Gicevic, Armina
https://orcid.org/0000-0002-0622-043X
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Becic, Ervina
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Imamovic, Belma
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Markovic, Damir
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Ziga-Smajic, Nermina
2020-11-27
2020-11-27
2020
Pharmacia
2603-557X
0428-0296
67
339-345
2020
10.1021/acs.jpcc.5b01590
10.1039/c3ta12212c
Structural and mechanical properties of graphene reinforced aluminum matrix composites.
P
Garg
author
2016
text
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2016
7
5
1461
1473
10.3390/nano4020267
S
Gutić
author
2016
2016
10.20964/2016.10.47
10.3390/nano9030313
10.1038/srep31920
10.1021/la203607w
10.1155/2013/150536
10.3390/ijms18112333
10.1039/C4RA13724H
10.1039/C7RA11964J
10.3897/pharmacia.67.e57242
https://pharmacia.pensoft.net/article/57242/
https://pharmacia.pensoft.net/article/57242/download/pdf/
https://pharmacia.pensoft.net/article/57242/download/xml/
This paper shows the use of membrane filters in adsorption of solution of tetracycline hydrochloride on graphene materials. The adsorption process was monitored at different wavelengths, different pH values at certain time intervals. The absorbances of the solutions were measured by UV-Vis spectrophotometry at two wavelengths (275 nm and 356 nm), and three pH values (pH 4, pH 7 and pH 10) every 90 minutes for 6 hours of monitoring, with constant stirring in an ultrasonic bath. The results showed decrease in absorbance at both wavelength and in all three pH values which proved the adsorption of tetracycline hydrochloride on GO and rGO. The largest decrease in absorbance was 98.1%. The most suitable pH value for adsorption was pH 4. This paper used a unique approach to filtration through membrane filters, which in the future could lead to the development of membrane filters based on graphene materials.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
spectrophotometry
antibiotics
purifiers
nanomaterial
Application of membrane filters in determination of the adsorption of tetracycline hydrochloride on graphene oxide
Research Article
10.3897/pharmacia.67.e53842
2020-11-27
pharmacia
National Centre of Infectious and Parasitic Diseases, Sofia, Bulgaria
author
Marinov, Radoslav
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria
author
Markova, Nadezhda
National Centre of Infectious and Parasitic Diseases, Sofia, Bulgaria
author
Krumova, Stefka
Sofia University St. Kliment Ohridski, Sofia, Bulgaria
author
Yotovska, Kamelia
The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
author
Zaharieva, Maya
https://orcid.org/0000-0001-8548-5649
National Centre of Infectious and Parasitic Diseases, Sofia, Bulgaria
author
Genova-Kalou, Petia
2020-11-27
2020-11-27
2020
Pharmacia
2603-557X
0428-0296
67
325-337
2020
10.1002/1099-1573(200012)14:8%3C604::AID-PTR678%3E3.0.CO;2-L
Synergism between the compound dichloroflavan and chalcone against poliovirus in vitro.
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10.1016/S0966-842X(99)01678-9
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10.3390/molecules200813563
Synthesis and Medicinal Significance of Chalcones – a review.
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Synthesis and utility of naphthalen-benzofuran chalcone in the synthesis of new pyrazole, isooxazole, thiazole, pyrimidine, pyran, pyridine and different azide derivatives with antiviral and antitumor activity.
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10.3897/pharmacia.67.e53842
https://pharmacia.pensoft.net/article/53842/
https://pharmacia.pensoft.net/article/53842/download/pdf/
https://pharmacia.pensoft.net/article/53842/download/xml/
Chalcones (natural or synthetic derivatives) are aromatic ketones possessing a central backbone that form a core for variety important compounds with different substitutions. Recent scientific advances show that chalcones exhibit different bio-medical activities, including antiviral, which is related to the variety substitutions. This review provides general information on the origin, sources, virucidal and direct antiviral properties of chalcones in vitro, as well as a brief overview of the possible application and molecular modes of action of these compounds. The antiviral effect of chalcones probably results from the disruption of the different stage of viral replication cycle, inhibition of viral or cell enzymes, induction of apoptosis and others. Structural requirements for antiviral activities vary according to the mechanisms of action. Based on the published information, it could be considered that synthetic chalcones are very perspective antiviral candidates and deserve further studies for elucidating of their pharmacological potential.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
antiviral effect
chalcones
in vitro
synthetic derivatives
Antiviral properties of chalcones and their synthetic derivatives: a mini review
Review Article
10.3897/pharmacia.67.e57981
2020-11-27
pharmacia
Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh
author
Sharmin, Suriya
Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh
author
Sohrab, Md. Hossain
Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh
author
Moni, Fatema
Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh
author
Afroz, Farhana
Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh
author
Rony, Satyajit Roy
https://orcid.org/0000-0002-6884-2904
Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh
author
Akhter, Shammi
2020-11-27
2020-11-27
2020
Pharmacia
2603-557X
0428-0296
67
383-391
2020
10.5958/2393-9087.2016.00031.5
A nonsteroidal antiinflammatory drug: aceclofenac.
SA
Arslan
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Validation of UV-Spectrophotometric and RP-HPLC methods for the simultaneous analysis of paracetamol and aceclofenac in marketed tablets.
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Ashraful Islam
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Stability indicating HPLC method for simultaneous determination of drotaverine and aceclofenac.
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Azhlwar
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International Journal of Pharmacy and Pharmaceutical Sciences
2011
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Method development and validation of stability indicating method for assay of diacerein and aceclofenac by HPLC.
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Badgujar
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Asian Journal of Chemistry
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10.3797/scipharm.1108-04
A new RP-HPLC-UV method for the simultaneous quantification of aceclofenac in bulk & its tablets.
PM
Chatrabhuji
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2015
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Analytical Chemistry: An Indian Journal
2015
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Simultaneous estimation of thiocolchicoside and aceclofenac in pharmaceutical dosage form by spectrophotometric and LC method.
SS
Chitlange
author
2010
text
Der Pharmacia Lettre
2010
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Development and validation of a RP-HPLC-PDA method for simultaneous estimation of drotaverine and aceclofenac in a combined dosage form.
V
Choudhari
author
2010
text
International Journal of Research in Pharmaceutical Sciences
2010
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253
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Development and validation of stability-indicating RP-HPLC-PDA method for simultaneous analysis of thiocolchicoside and aceclofenac in pharmaceutical dosage form.
VP
Choudhari
author
2011
text
Journal of Pharmacy Research
2011
4
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10.1081/DDC-120003453
Method development and validation for simultaneous estimation of drotaverine hydrochloride and aceclofenac in tablet dosage form by RP-HPLC.
S
Gandhi
author
2010
text
International Journal of Pharmaceutical Sciences Review and Research
2010
4
3
49
52
Development and validation of stability indicating HPLC method for determination of diacerein and aceclofenac as bulk drug and in tablet dosage form.
SP
Gandhi
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2011
text
International Journal of Research in Pharmacy and Chemistry
2011
1
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10.5958/2231-5659.2015.00021.1
10.1155/2014/643027
Simultaneous estimation of aceclofenac and paracetamol in solid dosage form by RP-HPLC Method.
D
Gharge
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2010
text
International Journal of ChemTech Research
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942
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Reverse phase HPLC method for determination of aceclofenac and paracetamol in tablet dosage form.
VP
Godse
author
2009
text
Asian Journal of Research in Chemistry
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10.1016/S0014-827X(02)01271-5
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MF
Hossain
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text
Der Pharma Chemica
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Development and validation of new analytical method for the simultaneous determination of five different drug substance in drug product using sub two microncolumn.
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Jadhav
author
2016
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World Journal of Pharmaceutical Research
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1029
1035
10.1365/s10337-008-0797-x
Development and validation of RP-HPLC-DAD method for estimation of aceclofenac and rabeprazole sodium in bulk and combined dosage form.
RN
Jinde
author
2013
text
Research Journal of Pharmacy and Technology
2013
6
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10.1080/00032710802515086
10.1365/s10337-008-0829-6
RP-LC gradient elution method for simultaneous determination of thiocolchicoside, aceclofenac and related impurities in tablet formulation.
PA
Karbhari
author
2014
text
Journal of Pharmacy & BioAllied Sciences
2014
6
246
252
Reversed phase high performance liquid chromatographic method for simultaneous estimation of pregabalin and aceclofenac in tablet formulation (Acenac-N).
SG
Khanage
author
2014
text
Journal of Reports in Pharmaceutical Sciences
2014
3
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184
192
Reversed-phase HPLC determination of aceclofenac in bulk powder and its pharmaceutical tablets.
AC
Kumar
author
2008
text
Asian Journal of Chemistry
2008
20
1
811
813
Validated RP-HPLC method for the simultaneous estimation of aceclofenac and diacerein in bulk and formulation.
RS
Kumar
author
2010
text
International Journal of PharmTech Research
2010
2
1
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944
A validated reversed phase HPLC-method for the determination of aceclofenac and tizanidine in tablets.
RS
Kumar
author
2010
text
Asian Journal of Pharmaceutical Research and Health Care
2010
2
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10.4103/0250-474X.26672
Development and implication of methodology for aceclofenac in pure and pharmaceutical formulations by microwave assisted spectrophotometry.
A
Mumtaz
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2013
text
Pakistan Journal of Pharmaceutical Sciences
2013
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10.15436/2377-1313.14.001
10.4172/2471-2698.1000122
A simple liquid chromatographic method for simultaneous determination of aceclofenac, methyl salicylate, and benzyl alcohol in pharmaceuticals.
APG
Nikalje
author
2018
text
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Development and validation of a RP-HPLC method for simultaneous estimation of diacerein and aceclofenac in pharmaceutical dosage form.
NK
Nyola
author
2012
text
Research and Reviews: Journal of Pharmaceutical Analysis
2012
1
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10.29161/PT.v7.i2.2019.55
10.1155/2013/242868
Standardization an a RP-HPLC method for the estimation of Aceclofenac in dosage form.
K
Paul
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text
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2011
1
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Method development and validation for simultaneous estimation of drotaverine hydrochloride and aceclofenac in bulk and formulation by RP-HPLC.
P
Pinnamaneni
author
2015
text
World Journal of Pharmacy and Pharmaceutical Sciences
2015
4
1351
1358
Analytical method development and validation of simultaneous estimation of paracetamol, aceclofenac and serratiopeptidase by RP-HPLC.
B
Podili
author
2017
text
International Journal of Ophthalmology & Visual Science
2017
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Development and validation of a novel stability indicating RP-HPLC method for simultaneous determination of Aceclofenac and Misoprostol in bulk and from their combined dosage form.
KE
Pravallika
author
2016
text
Der Pharm Lettre
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10.13005/ojc/330334
Development and validation of RP-HPLC method for Simultaneous determination of paracetamol, aceclofenac sodium and chlorzoxazone in combined dosage form.
P
Ravisankar
author
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text
World Journal of Pharmacy and Pharmaceutical Sciences
2013
3
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681
10.7897/2230-8407.04635
Development and validation of a reverse phase HPLC method for the determination of Aceclofenac in bulk and pharmaceutical dosage forms.
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Ravisankar
author
2013
text
International Journal of Research IN Pharmacy and Chemistry
2013
3
483
494
Advance simultaneous determination of paracetamol, thiocolchicoside and aceclofenac in tablets by reverse phase high performance liquid chromatography.
RV
Rele
author
2014
text
Der Pharmacia Sinica
2014
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10.1055/s-0033-1361128
Development and validation of RP-HPLC method for the simultaneous estimation of diacerein and aceclofenac in dosage form.
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Sekar
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text
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Simultaneous estimation of paracetamol and aceclofenac in combined dosage forms by RP-HPLC method.
PS
Selvan
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2007
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Asian Journal of Chemistry
2007
19
2
1004
1010
Validated spectroscopic method for estimation of aceclofenac from tablet formulation.
R
Shah
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2008
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430
432
10.1093/chromsci/46.7.649
10.1186/2228-5547-3-3
Reversed-phase high-performance liquid chromatographic and mass spectrophotometric methods for simultaneous determination of paracetamol, aceclofenac and tramadol in combined tablet dosage form in presence of its degradation products.
G
Sharma
author
2012
text
Asian Journal of Research in Chemistry
2012
5
7
854
858
A validated reversed phase-high performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of aceclofenac drug substance and its related traces impurities in the solid dosage form.
OD
Sherikar
author
2011
text
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2011
3
2
547
554
HPLC Method for the simultaneous determination of paracetamol, aceclofenac and tizanidine hydrochloride.
V
Somasekhar
author
2011
text
Asian Journal of Research in Chemistry
2011
23
4
1651
1654
Development of validated RP-HPLC method for bosentan in formulation and its application to in vitro interaction study with aceclofenac.
A
Suganthi
author
2014
text
World Journal of Pharmaceutical Research
2014
3
2897
2909
Stability indicating HPLC method for simultaneous determination of diacerein and aceclofenac.
A
Suganthi
author
2010
text
Research Journal of Pharmacy and Technology
2010
3
2
600
603
10.1155/2010/323410
Validation of UV spectroscopic method of analysis for assay and dissolution of aceclofenac tablet.
KR
Valambhia
author
2013
text
World Journal of Pharmacy and Pharmaceutical Sciences
2013
2
5
3977
3983
2005
2005
Validation of analytical procedures: text and methodology Q2 (R1) (2005) International conference on harmonization, Geneva, Switzerland. https://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1__Guideline.pdf
Method development and validation for the simultaneous estimation of aceclofenac and paracetamol in solid dosage form by using RP–HPLC.
B
Ventrapragada
author
2014
text
International Journal of Biopharm
2014
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Determination of aceclofenac by GC.
WA
Zeng-ri
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text
Qilu Pharmaceutical Affairs
2010
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468
470
10.3897/pharmacia.67.e57981
https://pharmacia.pensoft.net/article/57981/
https://pharmacia.pensoft.net/article/57981/download/pdf/
https://pharmacia.pensoft.net/article/57981/download/xml/
A reverse phase liquid chromatographic method for estimation of Aceclofenac in bulk drug and tablet dosage form was developed and validated. The chromatographic conditions to achieve the highest performance parameters using octylsilyl column with guard filter were optimized. The separation was carried out using a mobile phase containing 10 mM Phosphate Buffer, pH 2.1 and methanol (30:70% v/v) pumped at a flow rate of 1.0 mL/min with detection at 272 nm. The method was shown to be linear in 19.8–148.5 μg/mL concentration range (regression coefficient of 0.999). The limit of detection (LOD) and limit of quantification (LOQ) was found to be 0.0692 μg/mL and 0.2076 μg/mL, respectively. The accuracy of the method was assessed by adding fixed amount of pre-analyzed sample to different standard solutions (80%, 100%, and 120% of the tested concentration) in triplicate. The percentage mean recoveries were 97.91% to 100.39% with %RSD values of 0.64–0.79. The method was found to be precise with %RSD value of 1.13 and 1.60 for intraday and interday precision study, respectively. The method specificity and robustness were also established. New and sensitive HPLC method for estimation of Aceclofenac has been developed, in respect to the reviewed analytical methods.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Aceclofenac
Octylsilyl column
10 mM Phosphate Buffer
Method validation
Robustness
Simple RP-HPLC method for Aceclofenac quantitative analysis in pharmaceutical tablets
Research Article
10.3897/pharmacia.67.e59319
2020-11-27
pharmacia
Mutah University, Alkarak, Jordan
author
Al-Nadaf, Afaf
Mutah University, Alkarak, Jordan
author
Awadallah, Areeg
2020-11-27
2020-11-27
2020
Pharmacia
2603-557X
0428-0296
67
397-403
2020
10.4103/0973-1296.203975
10.1016/S0378-8741(03)00283-6
10.1016/S0378-8741(00)00215-4
10.1016/j.ctcp.2010.05.001
10.1080/01947640152750955
10.1016/j.ctim.2019.102255
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10.1111/j.1556-4029.2009.01252.x
10.3389/fphar.2020.00046
10.1016/j.jflm.2018.08.003
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10.3122/jabfm.19.6.566
10.4314/ajtcam.v8i5S.8
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10.1111/j.1365-2125.2012.04350.x
10.1016/j.ctim.2018.09.012
10.4172/2327-5162.1000130
J
Swerdlow
author
2000
2000
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2003
WHO (2003) Traditional medicine. http://www.who.int/mediacentre/factsheets/2003/fs134/en/ [16 Nov 2016]
2005
2005
WHO (2005) WHO global atlas of traditional, complementary and alternative medicine. In: Ong CK, Bodeker G, Grundy C, Burford G, Shein K (Eds) Map Volume. Geneva, World Health Organization.
A pilot study to assess students’ perceptions, familiarity, and knowledge in the use of complementary and alternative herbal supplements in health promotion.
C
Zimmerman
author
2012
text
Alternative Therapies in Health and Medicine
2012
18
5
28
33
10.3897/pharmacia.67.e59319
https://pharmacia.pensoft.net/article/59319/
https://pharmacia.pensoft.net/article/59319/download/pdf/
https://pharmacia.pensoft.net/article/59319/download/xml/
Background: Conventional medication; traditional or folk medicine as well as herbal medicines; have been widely used since ancient times. In Jordan traditional medicines are freely available and self-prescribed as well. No previous study has investigated believes and knowledge with regards to herb-drug interaction in Mutah geographical in Jordan. Objectives: This study aims to investigate and evaluate knowledge and attitude about self-medication using herbal medicine and its drug interaction among the public in Mutah. Materials and methods: A questionnaire-based survey conducted. Survey took place in different locations in the Mutah region in Al-Karak city from Jordan, during December 2017 and January 2018. Data analyzed by SPSS. Results: The majority of responders (82%) use self-prepared herbal preparation. While (56%) go for self-medication rather than visiting a physician. About (63%) participants responded that physicians didn’t ask them about if they use any herbal medication and about (50%) not willing to tell their physician about their self-medication. Knowledge about herbs gained from the family in the first place (38%); TV (21%) and friends (15%). Almost (79%) responders agree that herbs are safer and easier to be used compared to physician-prescribed medicine. Conclusion: A high belief in herbal therapy use. Responders gain their knowledge primarily from their families which they obtain their knowledge primarily from their tradition. They are not aware of their interaction with other therapies. Care-givers in this region should be aware of the high percentage of herbal medicine use and are encouraged to discuss potential use with their patients to avoid future complications.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Awareness
Attitude
Herbal medicine
Herb-Drug interaction
Mutah.
Evaluation for the level of knowledge about herbal medicine use within people and university students in Mutah region
Research Article
10.3897/pharmacia.67.e52568
2020-11-27
pharmacia
National University of Pharmacy, Kharkov, Ukraine
author
Rybalkin, Mykola
National University of Pharmacy, Kharkov, Ukraine
author
Khokhlenkova, Natalia
National University of Pharmacy, Kharkov, Ukraine
author
Azarenko, Julia
National University of Pharmacy, Kharkov, Ukraine
author
Diadiun, Tetiana
https://orcid.org/0000-0002-5742-5443
National University of Pharmacy, Kharkov, Ukraine
author
Kovalenko, Svitlana
2020-11-27
2020-11-27
2020
Pharmacia
2603-557X
0428-0296
67
393-396
2020
Combined use of antifungal agents and probiotics in combustiology for the treatment and prevention of candidiasis and irritable bowel syndrome.
SK
Borshch
author
2011
text
Modern Gastroenterology
2011
4
30
39
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10.1016/j.diagmicrobio.2012.02.001
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Dissemination of candidiasis, general characteristics of the pathogen, features of laboratory diagnostics.
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Golubka
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Candida albicans vaccines.
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17
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Rybalkin
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2014
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MV
Rybalkin
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2017
text
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28
108
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10.4103/0976-237X.86454
10.3897/pharmacia.67.e52568
https://pharmacia.pensoft.net/article/52568/
https://pharmacia.pensoft.net/article/52568/download/pdf/
https://pharmacia.pensoft.net/article/52568/download/xml/
Therapeutic effect of C. albicans proteins in concentrations 1, 2, 3, 4 and 5 mg/ml has been examined in white mice. Animals were infected i.p. with suspension of C. albicans strain CCM 335–867 in the amount of 20 million cells per 1 ml volume. After 5 days and repeatedly after 14 days mice were injected s.c. in the upper part of right hind paw with proteins of Candida cells of volume 0.2 ml. 14 days after each injection, the determination of the animal body protective functions has been carried out by the titer of specific antibodies during immunoassay. According to the data obtained, it has been found that in the s.c. administration, after the first and second injection with C. albicans protein of concentration 1, 2, 3, 4 and 5 mg/ml antibody titers increased 2 times, indicating that there is no stimulation of immune protection.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
antigen
candidiasis
dose
immunity
therapy
vaccine
Determination of Candida albicans proteins concentration by enzyme-linked immunosorbent assay method at subcutaneous introduction in candidiasis therapy
Research Article
10.3897/pharmacia.68.e53631
2021-01-07
pharmacia
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Peleshok, Kateryna
Replek Farm Ltd., Skopje, Republic of North Macedonia
author
Piponski, Marjan
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Ajie, Elizabeth Adaeze
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Poliak, Olha
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Zarivna, Nadiya
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Denefil, Olha
I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Logoyda, Liliya
2021-01-07
2021-01-07
2021
Pharmacia
2603-557X
0428-0296
68
1
43-51
2021
10.1093/jaoac/94.2.498
10.1016/j.trac.2012.03.013
10.1016/j.jchromb.2010.07.026
2005
2005
Guideline Q2(R1) (2005) Validation of Analytical Procedure: methodology, International Conference on Harmonisation. http://www.ich.org
UV-Spectrophotometric methods for estimation of valsartan in bulk and tablet dosage form.
KR
Gupta
author
2010
text
International Journal of ChemTechResearch
2010
2
985
989
10.1016/S0021-9673(02)01247-5
10.1080/10826076.2017.1373672
10.1093/jaoac/93.3.882
10.22159/ijap.2018v10i4.24528
10.22159/ijap.2018v10i4.24528
10.22159/ijap.2018v10i1.22805
10.22159/ijap.2018v10i2.23195
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10.22159/ijap.2018v10i4.24528
10.22159/ijap.2017v9i6.21616
Development of Methodology for Identification of Captopril in Medicines.
L
Liliya
author
2016
text
Asian Journal of Pharmaceutics
2016
10
3
168
171
10.22159/ijap.2019v11i4.32584
10.22159/ijap.2019v11i4.32420
Reversed phase HPLC analysis of valsartan in pharmaceutical dosage forms.
VB
Raju
author
2011
text
IjCEPr
2011
2
56
60
10.1002/bmc.1599
Simultaneous UV Spectrophotometric Method for Estimation of Losartan Potssium and Amlodipine Besylate in Tablet Dosage Form.
RP
Priyanka
author
2009
text
Asian Journal of Research in Chemistry
2009
2
1
183
187
10.1365/s10337-009-0950-1
10.1016/S0731-7085(02)00360-6
Simultaneous quantification of gabapentin, sulfamethoxazole, terbutryn, terbuthylazine and diuron by uv-vis spectrophotometer.
A
Goswami
author
2018
text
Biointerface Research in Applied Chemistry
2018
8
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10.33263/BRIAC95.305310
2003
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Q1A (R2) (2003) Feb ICH Harmonized Tripartite Guideline. Geneva.
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10.3897/pharmacia.68.e53631
https://pharmacia.pensoft.net/article/53631/
https://pharmacia.pensoft.net/article/53631/download/pdf/
https://pharmacia.pensoft.net/article/53631/download/xml/
Aim of this work was to develop the first simple, rapid, green, economical and selective HPLC method for simultaneous quantification of the cited drugs in their challenging binary mixture. The work was motivated by the global trends towards sustainable chemistry in designing eco-friendly mobile system without affecting the analysis parameters. The proposed method was subjected to a greenness profiles using some metrics as Eco-scale. Materials and methods. This was accomplished under the following chromatographic conditions: HPLC column Discovery C18 (4.6 mm i.d. × 150 mm, 5 μm), column temperature 30 °C, flow rate 1.0 mL/min, mobile phase composed of 20% acetonitrile, 80% of 0.16% ammonium acetate and 0.2% of 1.5 M tetramethylammonium hydroxide (V/V) and signal monitoring at a wavelength of 225 nm and 237 nm. Results. A conventional mixture of acetonitrile and 0.16% ammonium acetate was tried in different ratios, but the drugs were not well separated. The shortest aliphatic chain cationic ion pair reagent tetramethylammonium hydroxide should not be exchanged with other type similar with this, like tetramethylammonium hydrogen sulfate, it did not work to our experiments. Increasing salt concentration, ammonium acetate, more than 0.2%, pushes the peak of atenolol closer to dead volume, which is negative. Atenolol in their methods for multicomponent mixtures elutes in dead volume, or when retained longer, much stronger, hydrophobic mobile phase should be used if valsartan should be seen in same chromatogram at dissent time. The 237 nm can be chosen as compromise signal for nearly equal peaks height with high sensitivity is not essential. The 225 nm signal shows much higher sensitivity for atenolol and less increase for valsartan peaks, which can be used when higher sensitivities will be essential. Linearity was examined and proven at different concentration levels in the range of working concentration of valsartan (0.16–0.96 mg/mL) and atenolol (0.2–1.20 mg/mL). The high value of recoveries obtained for valsartan and atenolol indicates that the proposed method was found to be accurate. The results of proposed method found to be an excellent green analysis with a score of 84. Conclusion. A new fast, simple and green, but selective, accurate, precise and robust HPLC-UV method for simultaneous determination of valsartan and atenolol in newly formulated dosage form was developed and many possible variations of the same were suggested. The developed method for the simultaneous quantification of valsartan and atenolol in their challenging binary mixture offers simplicity essential for quality control of a large number of samples in short time intervals, which is necessary for routine analysis. The method was subjected to greenness profile assessment.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Valsartan
Atenolol
HPLC-UV
Method Development
Validation
Green chemistry
Novel HPLC-UV method for simultaneous determination of valsartan and atenolol in fixed dosage form; Study of green profile assessment
Research Article
10.3897/pharmacia.68.e52119
2021-01-07
pharmacia
Institute of Organic Chemistry, NAS of Ukraine, Kyiv, Ukraine
National Technical University of Ukraine "Igor Sikorsky Kyiv Polytechnic Institute", Kyiv, Ukraine
author
Golembiovska, Оlena
Zaporizhzhya State Medical University, Zaporozhye, Ukraine
author
Voskoboinik, Oleksii
Zaporizhzhya State Medical University, Zaporozhye, Ukraine
author
Berest, Galina
Zaporizhzhya State Medical University, Zaporozhye, Ukraine
author
Kovalenko, Sergiy
I. Horbachevsky Ternopil State Medical University, Ternopil, Ukraine
author
Logoyda, Liliya
2021-01-07
2021-01-07
2021
Pharmacia
2603-557X
0428-0296
68
1
53-59
2021
10.14412/1996-7012-2010-609
10.3949/ccjm.69.Suppl_1.SI31
Comparative characteristics of the therapeutic action of the 4-(3-methyl-2-oxo-2Н-[1,2,4]triazino[2,3-с]quinazolin-6-yl)butanoic acid (DSK-38 compound) and diclofenac on blood biochemical parameters on a model of adjuvant arthritis.
VV
Grib
author
2015
text
Kuban Scientific Medical Journal
2015
5
154
43
47
Analytical methods for residual solvents determination in pharmaceutical products.
K
Grodowska
author
2010
text
Acta Poloniae Pharmaceutica – Drug Research
2010
67
1
13
26
10.1023/A:1022693516409
1995
1995
ICH Q2 (1995) ICH Q2 (R1) Validation of Analytical Procedures: Text and Methodology, EMA/CPMP/ICH/381/1995.
2006
2006
ICH Q3C (2006) ICH Q3C (R6) Guideline on Impurities: Guideline for Residual solvents, EMA/CHMP/ICH/82260/2006.
10.1016/j.jpba.2005.06.036
SI
Kovalenko
author
2012
2012
10.1039/9781849735346
The use of non-steroidal anti-inflammatory drugs and cyclooxygenase-2 inhibitors in the early 21st century.
AL
Nasanov
author
2003
text
Russian Medical Journal
2003
11
7
375
378
10.1124/pr.56.3.3
GI
Stepanyuk
author
2013
2013
10.1096/fj.03-0645rev
10.3897/pharmacia.68.e52119
https://pharmacia.pensoft.net/article/52119/
https://pharmacia.pensoft.net/article/52119/download/pdf/
https://pharmacia.pensoft.net/article/52119/download/xml/
Aim. The aim of study was to develop and validate a simple, precise and accurate method using gas chromatography for analysis of residual solvents – acetone and 2-propanol – in quinabut API. Materials and methods. All experiments were performed on a gas chromatographic system equipped with FID detector (Shimadzu GC System) using the DB-624 (30 m × 0.32 mm ID, 3.0 μm film sickness) column as stationary phase. Nitrogen was used as carrier gas with flow rate 7.5 mL/ min. Split ratio was 1:5, injector temperature was 140 °C, detector temperature was 250 °C, oven temperature was programmed from 40 °C (2 min) to 50 °C at 1 °C/min and then increased at a rate of 15 °C/min up to 215 °C; and maintained for 2 min. All solutions were prepared using water as diluent. Results. This proposed method is assessed for separation of residual solvent from quinabut with quantification. The obtained results are compared with the corresponding specified limits of ICH standard guidelines. The method validation was done by evaluating specificity, limit of detection (LOD) and limit of quantitation (LOQ), linearity, accuracy, repeatability, ruggedness, system suitability and method precision of residual solvents as indicated in the ICH harmonized tripartite guideline. The separation between acetone and 2-propanol peaks is 2.07. Hence method was found to be specific. The linear relationship evaluated across range of 15 to 180% for acetone and 2-propanol of ICH specified limit of residual solvents. The graphs of theoretical concentration versus obtained concentration are linear and the regression coefficients ‘R’ for residual solvents were more than 0.9968. The values of LOD and LOQ were much less than the lower limit of the concentration range and cannot affect the accuracy of the test. The technique was characterized by high intra-laboratory accuracy at concentrations close to the nominal acetone and 2-propanol concentration. All solutions were stable in water for at least 1 hour when stored at room temperature. Conclusion. A simple, specific, accurate, precise and rugged gas chromatography method was developed and validated for the quantification of residual solvents present in quinabut API through an understanding of the synthetic process, nature of solvents and nature of stationary phases of columns. The residual solvents acetone and 2-propanol were determined.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Gas Chromatography
Quinabut
Residual Solvents
Validation
Method development and validation for the determination of residual solvents in quinabut API by using gas chromatography. Message 2
Research Article
10.3897/pharmacia.68.e54581
2021-01-07
pharmacia
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Omeragic, Elma
https://orcid.org/0000-0002-5996-4926
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Marjanovic, Aleksandra
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Djedjibegovic, Jasmina
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Turalic, Amila
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Dedic, Mirza
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Niksic, Haris
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Lugudic, Aida
University of Sarajevo, Sarajevo, Bosnia and Herzegovina
author
Sober, Miroslav
2021-01-07
2021-01-07
2021
Pharmacia
2603-557X
0428-0296
68
1
35-42
2021
10.1186/s12970-019-0296-5
10.1016/j.jclinane.2009.12.011
10.1123/ijsnem.11.3.365
10.1111/j.1527-3458.2006.00250.x
10.1016/j.csm.2005.03.005
10.1177/0363546505280428
10.1111/sms.12336
10.1590/S1517-86922013000200009
10.1097/00042752-200301000-00007
BR
Davis
author
2015
2015
N
Dikić
author
2010
2010
10.1378/chest.09-0865
10.1007/s00421-013-2746-0
Use of acetaminophen in young subelite athletes.
M
Garcin
author
2005
text
The Journal of Sports Medicine and Physical Fitness
2005
45
4
604
647
10.1136/bjsm.2009.062166
10.1123/ijsnem.21.4.271
10.1136/bmjopen-2012-002090
10.1123/ijsnem.22.1.31
10.1111/sms.13275
10.1113/expphysiol.2013.075275
10.1080/0264041031000140581
10.1136/bjsports-2018-099510
10.1152/japplphysiol.00707.2009
10.1136/bjsm.2004.015842
10.1002/pds.1191
10.1093/her/18.1.98
10.5550/sgia.151101.en.005O
10.1136/bjsm.2009.059980
10.1348/135910705X43606
10.26582/k.48.1.11
E
Pašalić
author
1999
1999
10.1186/1550-2783-4-19
Effect Of Paracetamol And Acetylsalicylic Acid Intake On Short Term Anaerobic Performance.
D
Petrů
author
2017
text
Journal Of Physical Education And Sport (JPES)
2017
17
4
2669
2673
10.1002/art.21357
10.1016/j.yrtph.2006.05.005
10.1016/j.yrtph.2008.01.004
10.1016/S0735-6757(03)00100-1
10.1123/ijsnem.13.3.320
10.1177/036354659702500419
10.1152/ajpregu.00611.2010
10.1016/S1054-139X(01)00325-1
10.1249/01.mss.0000210209.40694.09
10.1123/ijsnem.2013-0087
10.3897/pharmacia.68.e54581
https://pharmacia.pensoft.net/article/54581/
https://pharmacia.pensoft.net/article/54581/download/pdf/
https://pharmacia.pensoft.net/article/54581/download/xml/
Objectives: Food supplements and medicines which are not on the list of prohibited substances of the World Anti-Doping Agency are included in the group of permitted pharmacological agents for athlete’s recovery. The aim of this study was to describe qualitatively and quantitatively food supplements (FS) and over-the-counter drugs use among athletes in the last six month. Methods: This was a cross sectional study. Data on food supplements and the over-the-counter drugs, usage were collected during 2018 by self-administered, anonymous questionnaire. Results: A total of 112 athletes completed the survey. A total of 51.8% (n = 58) athletes reported the use of food supplements. The use of medical supplements was reported by 50.0% (n = 56) of athletes, 26.8% (n = 30) reported using ergogenic supplements, 1.8% (n = 2) using of sports food and 4.5% (n = 5) using other supplements. The use of over-the-counter drugs was reported by 35.7% (n = 40) of athletes. The over-the-counter analgesic drugs were used by 95% (n = 38) of over-the-counter drug users. Concomitant administration two or more over-the-counter drugs was reported by 40% (n = 16) athletes. Doctors and coaches had no advisory role in the use of food supplements or over-the-counter drugs.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
athletes
drugs
food supplements
OTC analgesic drugs
sport
Prevalence of use of permitted pharmacological substances for recovery among athletes
Research Article
10.3897/pharmacia.68.e56987
2021-01-07
pharmacia
Medical University of Plovdiv, Plovdiv, Bulgaria
author
Kilova, Kristina
https://orcid.org/0000-0002-3074-1972
Medical University of Plovdiv, Plovdiv, Bulgaria
author
Mihaylova, Anna A.
https://orcid.org/0000-0002-7674-6074
Medical University, Sofia, Bulgaria
author
Peikova, Lily
https://orcid.org/0000-0002-8030-3479
2021-01-07
2021-01-07
2021
Pharmacia
2603-557X
0428-0296
68
1
9-14
2021
10.1186/s13643-018-0727-4
10.1007/s11096-019-00816-4
10.2146/ajhp170039
10.1016/j.hlpt.2020.06.005
10.1093/ajhp/56.14.1405
10.3390/medicina55070327
10.3233/JRS-2011-0513
10.2146/ajhp060392
10.1186/s40545-020-00210-w
10.1211/PJ.2020.20208063
10.1089/153056201316970902
10.1093/ajhp/60.24.2577
R
Cooper
author
2020
2020
10.14748/.v4i1.5511
Z
Dimitrova
author
2005
Pharmaceutical Care. University press Sv.
2005
15 pp
10.2146/ajhp090643
10.1016/j.sapharm.2020.05.030
CCRA recognizes pharmacy services.
S
Felix
author
2004
text
Pharm Post
2004
12
1
6
10.1331/JAPhA.2009.08110
2020
2020
FIP Health Advisory (2020) COVID-19 Pandemic: Guidelines for Pharmacists and the Pharmacy Workforce March 26. 1–29. https://www.fip.org/files/content/priority-areas/coronavirus/COVID-19-Guidelines-for-pharmacists-and-the-pharmacy-workforce.pdf
10.1016/j.sapharm.2020.04.013
10.1089/tmj.2005.11.460
10.1093/ajhp/59.8.716
10.1089/tmj.2007.0045
10.1016/j.sapharm.2020.07.001
10.1331/JAPhA.2009.08128
SA
Margolis
author
2008
2008
2014–2020
2014–2020
National Health Strategy (2014–2020) National Health Strategy. https://www.mh.government.bg/media/filer_public/2015/06/26/proekt-nacionalna-zdravna-strategiq-2014-2020.pdf
10.1016/j.sapharm.2020.07.008
Drug related problems identified by European community pharmacists in patients discharged from hospital.
EI
Paulino
author
2004
text
Pharmacy World & Science
2004
26
6
353
360
V
Petkova
author
2017
2017
2020a
2020a
PGEU PR (2020a) PGEU PR Community pharmacists in the frontline in the fight against the novel corona-virus disease (COVID-19). [13 March 2020] https://www.pgeu.eu/wp-content/uploads/2020/03/PR-Community-Pharmacists-in-the-frontline-in-the-fight-against-coronavirus.pdf
2020b
2020b
PGEU PR (2020b) PGEU PR Community pharmacists supporting patients and healthcare systems during COVID-19 [15 May 2020] https://www.pgeu.eu/wp-content/uploads/2020/05/PGEU-PR-Community-Pharmacists-supporting-patients-and-healthcare-systems-during-COVID-19-15-May.pdf
10.1093/ajh/hpaa057
10.1186/1471-2369-12-35
М
Stoycheva
author
2012
2012
10.3897/pharmacia.68.e56987
https://pharmacia.pensoft.net/article/56987/
https://pharmacia.pensoft.net/article/56987/download/pdf/
https://pharmacia.pensoft.net/article/56987/download/xml/
This article discusses the opportunities of information and communication technologies for providing pharmaceutical care in the context of the COVID-19 pandemic. The global COVID-19 pandemic has necessitated a reorientation of the concept of providing health care to large-scale implementation of information technologies to improve the quality of medical and pharmaceutical services for the population. It had a significant impact on pharmacy, which necessitated changes in the management of pharmaceutical care for patients. Rapid and urgent measures were taken by the pharmacists, who found themselves at the forefront. Telepharmacy has proven to be a successful solution in the fight against the virus in the conditions of physical distance. The introduction of modern information and communication technologies worldwide will create significant advantages for more efficient use of resources and management of pharmaceutical care.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
health care
pharmaceutical services
pharmacist
telemedicine
telepharmacy
Opportunities of information communication technologies for providing pharmaceutical care in the COVID-19 pandemic
Research Article
10.3897/pharmacia.68.e57819
2021-01-07
pharmacia
University of Babylon, Hillah, Iraq
author
Al-Rafyai, Hawraa
University of Babylon, Hillah, Iraq
author
Alwash, Mourouge
https://orcid.org/0000-0002-9732-7135
University of Babylon, Hillah, Iraq
author
Al-Khafaji, Noor S.K.
https://orcid.org/0000-0001-7127-9246
2021-01-07
2021-01-07
2021
Pharmacia
2603-557X
0428-0296
68
1
1-7
2021
10.3897/pharmacia.68.e57819
https://pharmacia.pensoft.net/article/57819/
https://pharmacia.pensoft.net/article/57819/download/pdf/
https://pharmacia.pensoft.net/article/57819/download/xml/
Aquatic environment contamination remains a foremost global public health hazards, and symbolizes a significant reservoir of releasing antibiotic resistant bacteria. The survival of Escherichia coli in aquatic environments serves as a potential reservoir of antibiotic resistance, encompassing but not restricted to a plasmid-mediated quinolone resistance (PMQR) mechanism. The current study aimed to detect the presence of the PMQR-qnrA gene in quinolone-resistant E. coli isolates. Sixty-one waterborne E. coli with known phylogroups/subgroups isolated from the Al-Hillah River in Babylon Province, Iraq, were screened for the phenotypic resistance to third-generation quinolones (levofloxacin and ofloxacin) and were further analysed for the presence of the qnrA gene using polymerase chain reaction (PCR). Fifty-seven (93.4%) of 61 E. coli isolates were levofloxacin-resistant, and 55 (90.2%) were ofloxacin-resistant. Among the 57 quinolone-resistant E. coli, 40 (65.57%) isolates were found to carry the PMQR-qnrA gene. Among the 40 qnrA-positive E. coli, 22 (36.1%) isolates were in phylogroup B2, followed by 8 (13.1%) isolates in phylogroup D, 6 (9.8%) isolates in phylogroup B1, and 4 (6.6%) isolates in phylogroup A. The presence of the PMQR-qnrA gene in E. coli belonging to phylogroup B2 and D reflects the need for routine monitoring of antibiotic resistance genes (ARGs) in the Al-Hillah River.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Aquatic environment
Escherichia coli
qnrA gene
Quinolone resistance
Quinolone resistance (qnrA) gene in isolates of Escherichia coli collected from the Al-Hillah River in Babylon Province, Iraq
Research Article
10.3897/pharmacia.68.e56715
2021-01-07
pharmacia
Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Marchyshyn, Svitlana
Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Slobodianiuk, Liudmyla
https://orcid.org/0000-0002-0400-1305
Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Budniak, Liliia
https://orcid.org/0000-0002-4869-1344
Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
author
Skrynchuk, Olha
2021-01-07
2021-01-07
2021
Pharmacia
2603-557X
0428-0296
68
1
15-21
2021
10.1016/j.mex.2018.05.014
10.1016/0031-9422(80)85059-X
10.7324/JAPS.2015.50305
10.1007/s11101-015-9414-4
RYu
Basaraba
author
2019
2019
10.1016/j.phytochem.2011.01.014
Determination of antioxidant activity of Crambe cordifolia.
SM
Bukhari
author
2013
text
World Applied Sciences Journal
2013
22
11
1561
1565
10.5505/tjb.2014.92005
10.3109/07388551.2014.924474
10.1016/S0168-1656(01)00278-4
10.22159/ijap.2019v11i6.35282
10.1016/S1520-765X(01)90115-4
10.17221/1379-CJFS
10.1006/abio.1993.1244
Determination of sugars and fructans content in Stachys sieboldii.
L
Husak
author
2018
text
International Journal of Green Pharmacy
2018
12
70
74
10.3897/pharmacia.66.e37893
10.1111/j.1541-4337.2008.00065.x
10.15587/2519-4852.2018.135132
10.15414/agrobiodiversity.2017.2585-8246.216-220
Microbiological production of citric and isocitric acids from sunflower oil.
SV
Kamzolova
author
2008
text
Food Technology and Biotechnology
2008
46
1
51
59
10.1007/s002530100804
Recovery of pyruvate from fermentation broth by using ion exchange resin chromatography.
Y
Li
author
2001
text
Journal of Wuxi University of Light Industry
2001
20
4
335
339
10.1021/jf011440s
10.1007/s40034-017-0101-4
Chemical composition and antioxidant, antimicrobial and haemolytic activities of Crambe cordifolia roots.
MA
Rashid
author
2018
text
Farmacia
2018
66
1
165
171
10.1080/14786410802611303
10.1007/BF02249689
10.22159/ijap.2019v11i5.33909
10.31688/ABMU.2018.53.4.06
Research of fatty acid composition of common centaury herbs and crosne herbs.
LI
Stoyko
author
2015
text
Journal of Siberian Medical Sciences
2015
6
1
9
10.4025/actasciagron.v33i4.8248
The accumulation of nutrients in underground parts of plants of the genus Crambe L. spp.
OM
Vergun
author
2018
text
Plant Introduction
2018
78
2
3
11
10.1016/j.indcrop.2012.07.046
10.3897/pharmacia.68.e56715
https://pharmacia.pensoft.net/article/56715/
https://pharmacia.pensoft.net/article/56715/download/pdf/
https://pharmacia.pensoft.net/article/56715/download/xml/
Crambe cordifolia Steven is a perennial herb and contains many biologically active substances, including amino acids, quercetin and glycosides of kaempferol. In continuation of the investigation of these plant compounds, it is advisable to study the qualitative composition and quantitative contents of carboxylic acids. Using a HPLC method the quantitative content of the following organic acids was identified and determined: pyruvic (40.66 mg/g), isocitric (12.88 mg/g), citric (8.71 mg/g), succinic (38.03 mg/g) and malic (0.75 mg/g). Among fatty acids the saturated and unsaturated acids were determined by the GC/MS method. The content of polyunsaturated fatty acids of the total fatty acids was 56.97%, saturated – 38.53% and monounsaturated – 4.50%. Linolenic and palmitic acids dominated among the determined 7 fatty acids, their content was 9.68 mg/g (47.87%) and 4.88 mg/g (24.14%). The results of the study show that Crambe cordifolia Steven leaves is a source of carboxylic acids.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
Crambe cordifolia Steven
fatty acids
GC/MS
HPLC
organic acids
Analysis of carboxylic acids of Crambe cordifolia Steven
Research Article
10.3897/pharmacia.68.e56543
2021-01-07
pharmacia
University of Chittagong, Chittagong, Bangladesh
author
Bulbul, Md Z.H.
University of Chittagong, Chittagong, Bangladesh
author
Chowdhury, Tasneem S.
University of Chittagong, Chittagong, Bangladesh
author
Mlisbah, Md M.H.
University of Chittagong, Chittagong, Bangladesh
author
Ferdous, Jannatul
University of Chittagong, Chittagong, Bangladesh
author
Dey, Sujan
Rajshahi University, Rajshahi, Bangladesh
author
Hasan, Imtiaj
https://orcid.org/0000-0002-4715-9022
Nagasaki International University, Nagasaki, Japan
author
Fujii, Yuki
Yokohama City University, Yokohama, Japan
author
Ozeki, Yasuhiro
https://orcid.org/0000-0002-2782-6158
University of Chittagong, Chittagong, Bangladesh
author
Kawsar, Sarkar
2021-01-07
2021-01-07
2021
Pharmacia
2603-557X
0428-0296
68
1
23-34
2021
10.1016/j.ijbiomac.2017.04.109
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Biological evaluation of some mannopyranoside derivatives.
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Evaluation of the antimicrobial activity and cytotoxic effect of some uridine derivatives.
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Kawsar
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https://pharmacia.pensoft.net/article/56543/
https://pharmacia.pensoft.net/article/56543/download/pdf/
https://pharmacia.pensoft.net/article/56543/download/xml/
Nucleoside derivatives are important therapeutic drugs and are the focal point in the ongoing search for novel, more potent drug targets. In this study, a new series of pyrimidine nucleoside i.e., uridine (1) derivatives were synthesized via direct method and evaluated for their antimicrobial potential activity. The title compound uridine (1) was treated with triphenylmethyl chloride in pyridine to give the 5´-O-(triphenylmethyl)uridine derivative (2), which was subsequently derivatized to create a series of 2´,3´-di-O-acyl analogs containing a wide variety of functionalities in a single molecular framework. In vitro antimicrobial functionality tests were determined against both human and plant pathogens by disc diffusion and food poisoned techniques. The chemical structures of the synthesized compounds were confirmed on the basis of their spectral, analytical, physicochemical data. The antimicrobial results indicated that the synthesized derivatives exhibited moderate to good antibacterial and antifungal activity; in particular, they were found to be more effective against fungal phytopathogens than against human bacterial strains. Compounds 7, 9, and 14 were of particular interest as they exhibited noteworthy antifungal and antibacterial properties. In vitro MTT assays revealed that compound 9 was effective against Ehrlich’s ascites carcinoma (EAC) cells, resulting in 7.12% and 1.34% cell growth inhibition at concentrations of 200 and 6.25 µg/ml, respectively. The IC50 value for compound 9 was rather high and found to be 1956.25 µg/ml. Structure-activity relationship (SAR) studies were also conducted to predict structural and pharmacokinetic properties. The findings of this study indicate that the different uridine derivatives are potentially useful antimicrobial agents for the advancement of future pharmaceutical research.
text/html
en_US
Bulgarian Pharmaceutical Scientific Society
uridine
synthesis
antimicrobial
anticancer
SAR studies
Synthesis of new series of pyrimidine nucleoside derivatives bearing the acyl moieties as potential antimicrobial agents
Research Article
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