Review Article |
Corresponding author: Reggie Surya ( reggie.surya@binus.edu ) Corresponding author: Fahrul Nurkolis ( fahrul.nurkolis.mail@gmail.com ) Academic editor: Valentina Petkova
© 2024 Reggie Surya, Nurlinah Amalia, William Ben Gunawan, Nurpudji Astuti Taslim, Marwan Ghafoor, Nelly Mayulu, Hardinsyah Hardinsyah, Rony Abdi Syahputra, Felicia Kartawidjajaputra, Gianluca Rizzo, Raymond Rubianto Tjandrawinata, Dionysius Subali, Rudy Kurniawan, Fahrul Nurkolis.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Surya R, Amalia N, Gunawan WB, Taslim NA, Ghafoor M, Mayulu N, Hardinsyah H, Abdi Syahputra R, Kartawidjajaputra F, Rizzo G, Tjandrawinata RR, Subali D, Kurniawan R, Nurkolis F (2024) Tempe as superior functional antioxidant food: From biomechanism to future development of soybean-based functional food. Pharmacia 71: 1-7. https://doi.org/10.3897/pharmacia.71.e116748
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Foods that have nutritional value along with additional health advantages are referred to as functional foods. Fruits, vegetables, and spices are rich sources of antioxidants, which can help prevent damage from free radicals and environmental stress. It has been demonstrated that consuming foods high in antioxidants lowers the risk of degenerative diseases such as cancer, emphysema, immunological deficiencies, respiratory disorders, heart disease, and stroke. It also lowers the risk of Parkinson’s disease and other inflammatory conditions. Traditional Indonesian fermented soybean-based food products, or soybeans and known as “tempe”, have been associated with a host of health benefits, including a lower risk of cardiovascular disease, a lower risk of cancer, improved bone health, and enhanced immunological function. This article investigates tempe’s potential as a meal with antioxidant properties and suggests a mechanism via which it can trigger the Nrf2-mediated antioxidant response. The study offers insights into the potential applications, development, and potentiation of tempe by synthesizing potential biomolecular pathways for its antioxidant actions at the cellular level.
fermented food, functional food, Nrf2-mediated antioxidant response, oxidative stress, soybean
The term “functional food” refers to food that provides health-related benefits in addition to its nutritional interests (
Consuming soybean (Glycine max), either fermented or unfermented, has been a typical characteristic of food culture in many Asian countries (
Tempe is a traditional fermented soybean-based food product from Indonesia (
This article aims to explore the potential of tempe as an antioxidant-functional food. Based on recent literature, we propose a mechanism by which tempe may exert antioxidant activities by activating the Nrf2-mediated antioxidant response. The novelty of this article is focused on providing a holistic approach regarding the role of tempe in modulating cellular antioxidant response, a topic that has been very little evoked in any previous studies. This article synthesizes the possible biomolecular mechanisms for the antioxidant activities of tempe at the cellular level, which have never been reported before. Finally, we also provide some insights into the further development, application, and potentiation of tempe as an antioxidant functional food.
Fermenting soybeans into tempe has many nutritional interests. Fungal activities allow the degradation of anti-nutritional compounds naturally present in soybean (such as anti-trypsin and phytic acid) and the hydrolysis of proteins into shorter polypeptides, thus improving the bioavailability of proteins and other nutrients (
Tempe is a potential source of antioxidants. Tempe flour-based products contain a higher level of flavonoids compared to wheat flour-based products (
The Nrf2-mediated signaling pathway is an essential cellular defense mechanism against oxidative and/or electrophilic stresses (
In addition to oxidative stress, a diverse array of stimuli can activate the Nrf2-dependent antioxidant response. Interestingly, many food components, particularly those with antioxidant activities, can activate Nrf2 by interacting with Keap1 and modifying its residues (mainly Cys-151), allowing the liberation and activation of Nrf2 to the nucleus (
There are several studies that show in vivo, in vitro clinical evidence of Tempe consumption, on lung health, cancer, liver, skeletal muscle recovery, anemia, bone health, malnutrition, gut health, obesity, type 2 diabetes mellitus, cardiovascular health, and Alzheimer’s disease (
Fig.
Proposed-biomechanism of tempe on the modulation of cellular antioxidant status via the Nrf2- dependent signaling pathway. Abbreviations: 3HAA: 3-hydroxyanthranilic acid; AKT: protein kinase B; ARE: antioxidant response element; GPx: glutathione peroxidase; GR: glutathione reductase; IFAs: isoflavone aglycones; IFGs: isoflavone glycosides; Keap1: Kelch-like ECH-associated protein 1; Nrf2: nuclear factor erythroid 2-related factor 2; PI3K: phosphoinositide 3-kinase; ROS: reactive oxygen species; RTK: receptor tyrosine kinase; SOD: superoxide dismutase.
3HAA is another antioxidant molecule derived from tryptophan during the fermentation of tempe (
In recent studies, tempe was demonstrated to improve cellular antioxidant status and induce the expression of cellular antioxidant enzymes, including catalase and superoxide dismutases (SOD1, SOD2, and SOD3) in different cellular models (
Health outcomes | Authors | Publication date | Key findings |
---|---|---|---|
Lung Health | Setiawan et al. | 2016 | Finds that soy-based supplements particularly tempeh illustrate effectiveness on Tuberculosis patients who are on antimicrobial therapy. |
Cancer | Nurkolis et al. | 2022 | Tempeh contains bioactive chemicals that function as anticancer agents inhibiting cell proliferation and angiogenesis, also works as an antioxidant, and induces apoptosis of cancer cells. |
Liver | Reggie et al. | 2020 | The study showed that the expression of cellular antioxidant enzymes, such as superoxide and catalase dismutases, in HepG2 cells, could be boosted. Thus, tempeh might be found to have the ability to protect liver cells, by strengthening their antioxidant resistance |
Skeletal Muscle Recovery | Gomes et al. | 2021 | This study illustrates that fermented soybean antioxidant activity reduces oxidative stress in skeletal muscle and improves performance when undergoing high-intensity exercises in rats, indirectly promoting a healthier muscle environment for the repair and regeneration process. |
Anemia | Sudargo et al. | 2013 | The study concludes that the bioavailability of iron in iron-fortified tempeh is increased. |
Obesity | Watti et al. | 2020 | Suggests that tempeh consumption at the amount of 150 grams a day for 28 days can practically reduce high-sensitivity C-reactive protein and increase levels of HDL cholesterol. |
Type 2 Diabetes Mellitus | Park et al. | 2010 | Fermented soybean has shown possible advantages for patients with type 2 diabetes, including reduced insulin resistance, improvement of glucose control, and delay or prevention of disease progression. |
Cardiovascular Health | Barus et al. | 2019 | Significant attention is given to soybean-containing foods because of their potential capacity for lowering the emergence and progression of many chronic diseases such as osteoporosis, cancer, Alzheimer’s disease, cardiovascular disease, and stroke. |
Alzheimer’s disease | Subali et al. | 2022 | Without the use of medication, tempeh can provide Alzheimer’s patients with the nutrition they require to naturally enhance their condition and overall health. |
Since tempe contains both probiotics and their nutrients generally known as prebiotics (fiber and other polysaccharides), tempe can be suggested to be a synbiotic food (
To potentiate the antioxidant activity of tempe, we also propose the combination of tempe with other ingredients that are known as Nrf2 activators, in particular spices with bioactive compounds (
In addition to having a promising potential as an antioxidant food, tempe also has the potential to be developed into an innovative healthy meal (
Taken together, we suggest the development of tempe as an antioxidant functional food based on the literature-based proposed mechanism. Such a development could support the potential of tempe in disease prevention, such as cancer and cardiovascular diseases. We propose that the future perspectives of tempe application as an antioxidant functional food should be articulated around integrating tempe into daily consumed foods and developing innovative tempe-based food products. Identification of different metabolites in tempe through foodomics or metabolomics would also be essential to perform to further explore the health-promoting compounds present in tempe.
Tempe is thought to be a superior source of antioxidants than unfermented soybeans. In fact, it was discovered that tempe extract has greater antioxidant activity than soybean extract. This article explores the possibility of tempe as an antioxidant-rich meal and proposes a method by which tempe may activate the Nrf2-mediated antioxidant response, which have never been reported before. The research synthesizes possible biomolecular routes for tempe’s antioxidant effects at the cellular level, providing insights into the prospective applications, development, and potentiation of tempe.
We want to express our gratitude to the Chairman of the Indonesian Association of Clinical Nutrition Physicians, Professor Nurpudji Astuti Taslim, MD., MPH., PhD., Sp.GK(K), and the President of the Federation of Asian Nutrition Societies (FANS), Professor Hardinsyah, Ph.D., for reviewing and providing suggestions, as well as input on the draft of this opinion article.
Not Applicable or Authors declare that this study is review type article, so there are no experiments in animals or humans that need to be declared.
There is no data related to this opinion article, the data were only sourced from the literature listed in this article.
RS, NAT, and FN: Contributed to the conceptualization with the design of the study/work, drafted the manuscript, edited-revised it, and approved the final version of the submitted manuscript. WBG, FN, MG, NM, RAS, FK, DS, RK, NA, GR, and RRT: Drafting the work and revising it critically for important intellectual content, analysis and/or interpretation of literature data. FN and RS: Software and Visualization. NAT, RRT, HH, NM, and FN: writing–review and editing, and supervision. All authors have read and agreed to the published version of the manuscript.