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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Material and methods
    • Materials
      • Animals
    • Methods
      • Analytical methods
      • Solubility of retinyl palmitate and vitamin E
      • Formulation of the Nanoemulsion (NE)
      • Preparation of surfactant and co surfactant mixture (Smix)
      • Preparation of the nanoemulsion (NE)
      • Preparation of the final formulation
      • Characterization of the prepared NE
      • Measurement of droplet size and charge
      • Assay of RP and VE in the selected formulae (FF1 and FF2)
      • Rheological study
      • Diffusion study
      • Skin irritation/corrosive potential test
      • Preliminary stability study at room temperature
      • Statistical analysis
  • Results and discussion
    • Method of analysis of Retinyl palmitate (RP) and vitamin E (VE)
    • Solubility study of retinol palmitate (RP) and vitamin E (VE)
    • Formulation of the Nanoemulsion (NE)
    • Characterization of NE
      • Particle size measurement and Zeta potential
      • Assay of RP and VE in FF1 and FF2
    • Rheological study
    • Diffusion study
    • Irritation study
    • Preliminary stability test at room temperature
  • Conclusions
  • Author contributions
  • References
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