Cytotoxicity of compound 4C is attributed to apoptosis not necrosis. A) Hep G2 cells were treated with compound 4C (4.62 µM) for 48 h, harvested, stained with AnnexinV-FITC/propidium iodide (PI), and analyzed for apoptosis (early: bottom right corner negative PI/positive annexin V; late: top right corner positive PI/positive annexin V) versus necrosis (top left corner positive PI/negative annexin V) using flow cytometry, B) mRNA level analysis of p53, BAX, Bcl-2, and caspases-3 genes, normalized over GAPDH, in control and compound 4C-treated Hep G2 cells. Values are given as mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01 as compared to control cells.