Changes in a) VEGF and b) HIF-1α gene expression in 4T1 cells treated with IC50 of EVE, 5FU, LiCl, the combination of EVE with 5FU, and the combination of 5FU with LiCl for 48 h. However, LiCl upregulated (p-value < 0.0001) and EVE single-treatment did not show significant change in VEGF relative expression. However, it reduced significantly by 5FU-LiCl (p-value < 0.05) and EVE-5FU (p-value < 0.01) than single therapies and control (p-value < 0.0001). HIF-1α was downregulated noticeably compared to the control in all treatment groups (p-value < 0.01) except LiCl. However, no significant difference was observed between individual and combinatorial treatments; Data are expressed as mean ± SD from 3 independent repetitions. *p-value < 0.05, **p-value < 0.01 and ***p-value < 0.0001 compared to the control.