a) 4T1 cell viability and b) Microscopic images of untreated 4T1 cells and treated cells after 48 h treatment with IC50 of EVE, 5FU, and LiCl as a single or combinational treatment. Dual-combinations generally demonstrated higher cytotoxicity than single and triple-combination therapies. EVE-5FU considerably enhanced the cytotoxicity of cells rather than EVE (p-value < 0.01) and 5FU (p-value < 0.0001). Also, 5FU-LiCl significantly increased it rather than 5FU and LiCl (p-value < 0.0001). Cell size and shape showed more intense changes in combination therapies than single treatments and control as well as detachment, elongation, and morphological disarray; Data are expressed as mean ± SD from 3 independent repetitions. **p-value < 0.01 and ***p-value < 0.0001