FMD improved the inhibitory effect of INT 747 on the growth of prostate cancer cells. (A) The cell viability assay was performed on cells growing in regular medium with DMSO (Control) or starvation medium (FMD) After 24 h, 48 h, or 72 h, 8 µM INT-747 was added when indicated. Later, viability was detected by the MTT assay. (B) Cell morphology of ,PC-3 cells after treatment with INT-747 for 48 h was imaged under an inverted microscope at 10 × to detect any changes. (C) and (D) representative images of the colony number in each group (top panel) and proliferation of single cell clones (bottom panel), stained with crystal violet.Scale bar: 80 µm.