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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Materials and, methods
    • Chemicals and their sources
    • Cell-culture and fasting mimicking condition
    • Cell viability assay
    • Anchorage-Independent Colony Formation Assay (CFA)
    • ROS detection by the nitrogen-blue tetrazolium assay
    • Transwell migration- assay
    • -Annexin V/PI apoptosis assay
    • Quantitative real-time PCR (qRT-PCR)
    • Statistical analysis
  • Results
    • INT 747 represses the cell viability of prostate cancer cells
    • The starvation conditions potentiate the antiproliferative effects of INT 747 in cultured cancer cells
    • Apoptotic effects of the combination of FXR activation and FMD on PC-3 cells
    • Effect of FXR activation and /or FMD on genes associated with cell proliferation
    • Involvement of ROS production in INT 747 and /or FMD-treated prostate carcinoma cells
    • FXR activation and FMD suppress in vitro cell migration of prostate cancer cells
  • Discussion
  • Conclusions
  • Conflict of interest
  • Acknowledgements
  • References
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