Compound 7 induces apoptosis in MCF-7 breast cancer cells. A) Fluorescence images of MCF-7 breast cancer cells showing fragmented nuclei after DAPI staining. Scale bar: 10 µm, B) Detection of DNA fragmentation by agarose gel electrophoresis. Cells were treated and genomic DNA was extracted and electrophoresed on 1.5% agarose gels. M: DNA molecular weight marker; C: vehicle-treated control cells; 5%: 5% DMSO-treated cells; 7: Compound 7-treated cells. Three experiments were performed with similar results.