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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Materials and methods
    • Materials and reagents
    • Optimization of HPLC conditions
    • Optimization of preparation method for test solution
      • Screening extraction solvent for test solution preparation by thin layer chromatography
      • Screening the ratio of EE tablet powder and extraction solvent for preparation of test solution
    • Method validation
      • Specificity
      • System suitability
      • Linearity
      • Lower limit of detection and lower limit of quantification
      • Precision and accuracy
  • Results and discussion
    • Optimization of HPLC conditions
      • Selection of UV wavelength for simultaneous detection of three targeted markers
      • Screening of mobile phase
    • Optimization of preparation method for test solution
      • Screening extraction solvent for test solution preparation by TLC
    • Screening the ratio of EE tablet powder and extraction solvent for test solution preparation
    • Method validation
      • System suitability
      • Specificity
      • Linearity, lower limit of detection and lower limit of quantification
    • Precision and accuracy
    • Analysis of EE tablet samples
  • Conclusion
  • Acknowledgements
  • References
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