Chromatogram obtained using Shimadzu Nexera XR UPLC system and mobile phase 50% metanol and 40% of 0.07% (V/V) perchloric acid, column Zorbax Rx C8 (4.6 mm i.d. × 250 mm, 5 μm), with 3-D UV contour diagram extracted ,analytes UV spectra and peak purity. First peak at about 6.1min is bisoprolol and peak at about 7.1min is enalapril.