"Factor name";Abbr.;Settings;Initial "(f1) Buffer pH";pH;"2.6 to 3.4";3.0 "(f2) Amount of PBS";PBS;"45 to 65 mL";"55 mL" "(f3) Amount of ACN";ACN;"35 to 55 mL";"45 mL" "(f4) Column temperature";Temp;"30 to 50 °С";"40 °С"