Diosgenin quantitative determination chromatogram in caltrop herb. 1 – diosgenin standard sample (5 µL / 2,5 µg); 3 – diosgenin standard sample (10 µL / 5 µg); 5 – diosgenin standard sample (15 µL / 7,5 µg); 7 – diosgenin standard sample (10 µL); 9 – diosgenin standard sample (5 µL); 2, 4, 6, 8 – solutions of caltrop herb samples.