Alignment of the target sequence of H. corymbosa with the corresponding sequences of H. diffusa, H. pinifolia, and H. tenelliflora. Note: The differential base between the target sequence of H. corymbosa and those of H. diffusa, H. pinifolia, or H. tenelliflora was T→C; thus, the 3ʹ end mismatch nucleotide of the Proofman probe was designed as A→G.