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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Materials and methods
    • Medicinal herbal extraction
    • Experimental design and optimization of the inhibitory effects on oral pathogens (S. mutans, S. oralis)
    • Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)
    • Optimization of mucoadhesive films
    • Preparation of mucoadhesive films
    • Ex vivo active compound buccal mucosa retention study
    • Quantification of active compounds of herbal extract using a high-performance liquid chromatography (HPLC) method
    • In vitro cytotoxicity assay
    • Accelerated stability test
    • Data analysis and optimization
  • Results and discussion
    • Optimization of the inhibitory effects on oral pathogens (S. mutans, S. oralis)
    • Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) from optimized herbal extract combinations and quantification of active compounds
    • Optimization of the mucoadhesive film formulation
    • Ex vivo active compound buccal mucosa retention study and cytotoxicity test
  • Conclusion
  • Acknowledgments
  • Additional information
  • References
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