E. coli cell yield obtained after IPTG induction and autoinduction of A Clones F8 and F10 and C Clones B11 and F11 B Non-reducing 15% SDS-PAGE of anti-C3 scFv eluates of clones F8 and F10. Lanes: M, molecular marker; 2 and 3: clone F8 eluates from IPTG induction; 4 and 5: clone F8 eluates from autoinduction; 6 and 7: clone F10 eluates from IPTG induction; 8 and 9: clone F10 eluates from autoinduction; D Non-reducing 15% SDS-PAGE of anti-C3 scFv eluates from clones B11 and F11. Lanes: M, molecular marker; 2, 3, 4: clone F11 eluates from IPTG induction; 5, 6, 7: clone F11 eluates from autoinduction; 8 and 9: eluates from culture growth medium of clone B11; 10 - cell lysate from IPTG induction of clone B11; 11 - cell lysate from autoinduction of clone B11. The relative protein molecular weight (kDa) was estimated with ImageJ software.

 
 
  Part of: Poryazova R, Cholakova G, Kapogianni A, Tsacheva I (2025) Selection of high-affinity single-chain antibodies to human C3 by phage display. Pharmacia 72: 1-8. https://doi.org/10.3897/pharmacia.72.e150821