Immunoblotting of the selected high-affinity scFv clones for the recognition of the intact C3 and its smaller fragments C3b, C3c, and C3d A NC with blotted proteins after non-reducing 15% SDS PAGE. Western blot B and dot blot C of antigens with selected anti-C3 scFv clones D NC with blotted proteins after reducing 15% SDS PAGE E Western blot of reduced antigens with selected anti-C3 scFv clones.

 
 
  Part of: Poryazova R, Cholakova G, Kapogianni A, Tsacheva I (2025) Selection of high-affinity single-chain antibodies to human C3 by phage display. Pharmacia 72: 1-8. https://doi.org/10.3897/pharmacia.72.e150821