Dot blot assay for detection of positive anti-C3 scFv antibodies selected in round III (clones B2-C11) and in round IV (clones E2-F11). 1 dot = mean value of duplicate measurement of integrated density of a sample normalized to a control. Statistical analysis was done with a paired Student t-test, error bars: mean ± SD, **** (p ≤ 0.0001).

 
 
  Part of: Poryazova R, Cholakova G, Kapogianni A, Tsacheva I (2025) Selection of high-affinity single-chain antibodies to human C3 by phage display. Pharmacia 72: 1-8. https://doi.org/10.3897/pharmacia.72.e150821