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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Materials and methods
    • Chemicals and reagents
    • Cell lines and culture
    • Study design
    • Ethical considerations and study settings
    • Anti-angiogenic ex vivo RAR assay
    • In vivo CAM assay
    • Free radical scavenging activity with DPPH assay
    • Cell viability in vitro assay
    • Gene expression analysis
    • Statistical analysis
  • Results
    • Ex vivo RAR assay of 2-NPHC
    • In vivo CAM assay of 2-NPHC
    • Free radical scavenging activity of 2-NPHC using the DPPH assay
    • Effect of 2-NPHC on HUVEC viability
    • Effect of 2-NPHC on the VEGF gene expression in HCT116 cell line
  • Discussion
    • Study limitations
  • Conclusion
  • References
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