Effect of routes of administration for alloxan with different doses on mice. A. Changes in blood glucose level; B. Changes in body weight; C. Survival/mortality rate. Data were expressed as mean ± S.E.M, n = 10–15 mice per group (male and female were used approximately equally). Comparisons between the groups were made by one-way analysis of variance (ANOVA), followed by a Tukey post-hoc test. ****p < 0.0001 vs. control group. I.V. = intravenous injection. I.P. = intraperitoneal injection.