Effect of Camellia flava (Pitard) Sealy flower extract on the degeneration of Islets of Langerhans and insulin resistance in alloxan-induced hyperglycemia model on Swiss albino mice

Huu Lac Thuy Nguyen; Thi Thu Phuong Ha; Ngoc Phuc Nguyen Nguyen; Nguyen Hoang Linh Phan; Dang Thuy Hien Nguyen; Van Dat Truong; Minh Nhut Truong; Thao-My Nguyen-Hoang; Thi Nghia Luu; Ngoc Phuc Nguyen; Thanh Binh Nguyen; Huynh Nhu Mai

doi:10.3897/pharmacia.71.e126255

Research Article

Pharmacia 71: 1-15
https://doi.org/10.3897/pharmacia.71.e126255 (23 Jul 2024)

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Article title
Abstract
Keywords
Introduction
Materials and methods
- Animals
- Plant collection and extraction
- Determination of total flavonoid content in Camellia flava flower extract
- Evaluation of the antioxidant activity of Camellia flava flower extract
  - 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity
- Determination of the route of injection for alloxan
- Experimental design for the evaluation of the hypoglycemic effect of Camellia flava on mice
- Evaluation of blood glucose levels
- Evaluation of HbA1c, insulin levels, and insulin resistance
  - HbA1c assay
  - Insulin assay
  - Evaluation of insulin resistance
- Evaluation of creatinine levels
- Evaluation of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels
  - AST assay
  - ALT assay
- Hematoxylin and eosin (H&E) staining
- Statistical analysis
Results
- Total flavonoid content in Camellia flava ethanolic flower extract
- Antioxidant activity of Camellia flava flower extract
- Effect of routes of administration for alloxan on mice
  - Blood glucose level
  - Body weight
  - Survival/mortality rate
- Effect of Camellia flava flower extract on blood glucose levels in alloxan-induced hyperglycemic mice
- Effect of Camellia flava flower extract on HbA1c, insulin levels, and insulin resistance in alloxan-induced hyperglycemic mice
- Effect of Camellia flava flower extract on body weights in alloxan-induced hyperglycemic mice
- Effect of Camellia flava flower extract on biochemical indices in alloxan-induced hyperglycemic mice
- Effect of Camellia flava flower extract on pancreases in alloxan-induced hyperglycemic mice
- Effect of Camellia flava flower extract on liver and kidney in alloxan-induced hyperglycemic mice
Discussion
Conclusion
Competing interests
CRediT authorship contribution statement
Acknowledgements
References
Supplementary material

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