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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Materials and methods
    • Extraction procedure
    • Survey of extraction conditions using the classical extraction method
    • Survey of extraction conditions using ultrasonic extraction method
    • Purification of mangiferin
      • Survey of solvents of liquid-liquid extraction
    • Analysis of mangiferin properties after purification
    • Preparation of HPTR
    • Experimental animals
    • Acute toxicity
    • Sub-chronic toxicity
    • Experimental design to investigate the effect of HPTR and mangiferin on acute hyperlipidemia
    • Serum chemistry analysis for sub-chronic toxicity
    • Hematoxylin & eosin (H&E) staining
    • Biochemical parameters and lipid profile measurements
    • Statistical analysis
  • Results
    • Survey of extraction conditions using classical and ultrasonic extraction methods
    • Survey of solvents of liquid-liquid extraction
    • Analysis of mangiferin properties after purification
    • General sign and behavioral analysis
    • Effect of HPTR and mangiferin on hematological parameters in sub-chronic oral toxicity tests
    • Effect of HPTR and mangiferin extract on serum biochemical parameters in sub-chronic oral toxicity tests
    • Histopathological observation
    • Effects of HPTR and mangiferin in a tyloxapol-induced acute hyperlipidemia mouse model
  • Discussion
  • Conclusion
  • CRediT authorship contribution statement
  • Declaration of competing interest
  • Acknowledgements
  • References
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