Effects of riboflavin on changes in pain threshold were investigated using the paw pressure test. Streptozotocin administration significantly induced mechanical hyperalgesia by reducing the mechanical threshold and enhancing paw withdrawal responses, while supplementation with Vitamin B2 effectively mitigated mechanical hyperalgesia. Significant differences compared to the control group (Normal) are denoted as *P < 0.05, while significant differences compared to the diabetic group (Diabetic) are denoted as ##P < 0.01. n=8 animals per group.