(a) Effect of lipase concentrations (2.5, 5 and 10 mg/mL) with 3 µL of 1.5 mg/mL a-naphthyl acetate and 45 µL of 1.0 mg/mL FBB. (b) Effect of a-naphthyl acetate (NA) concentrations (0.75, 1.0 and 1.5 mg/mL) with 3 µL of 5 mg/mL lipase and 45 µL of 1.0 mg/mL FBB. (c) Effect of FBB concentration (0.5 and 1.0 mg/mL) with 3 µL of 1.5 mg/mL a-naphthyl acetate and 3 µL of 5.0 mg/mL lipase.