The effect of ACE increased TGF-β production (CD4+TGF-β+) in mice fed with a high fat-fructose diet for 5 months. The expression of TGF-β (CD4+TGF-β+) of mice fed with HFFD and administration of ACE from flow cytometry analysis (Fig. 5G). The percentage of regulatory (CD4+IL-10+) of mice fed with HFFD and administration of ACE (Fig. 5H). Data are mean ± SD (n=5). N: normal fed mice (non-high fat-fructose diet), HFFD: high fat-fructose diet mice (w/o administration of ACE), D1: HFFD mice receiving ACE 104 mg/kg body weight, D2: HFFD mice receiving ACE 520 mg/kg BW, D3: HFFD mice receiving ACE 5200 mg/kg BW. The different notation on the chart was considered significantly different for each group at p < 0.05 and vice versa on DMRT post hoc test.