Administration of ACE increased IL-10 production (CD4+IL-10+) in mice fed with a high-fat, high-fructose diet for 5 months. The expression of CD4+IL-10+ in mice fed with HFFD and administered ACE was determined by flow cytometry analysis (Fig. 4E). The percentage of regulatory cells (CD4+IL-10+) in mice fed with HFFD and administered ACE (Fig. 4F) The data are mean SD (n = 5). N: normal-fed mice (non-high-fat-fructose diet); HFFD: high-fat-fructose diet mice (w/o administration of ACE); D1: HFFD mice receiving ACE 104 mg/kg body weight; D2: HFFD mice receiving ACE 520 mg/kg body weight; D3: HFFD mice receiving ACE 5200 mg/kg body weight. The different notation on the chart was considered significantly different for each group at p < 0.05 and vice versa on the DMRT pos hoc test.