Effect of TMNB on writhing in mice induced by 0.6% acetic acid. Six mice were included in the study and were injected with either 5% DMSO (vehicle), TMNB (at doses of 50, 100, 150, 200 µg/kg i.p.), or acetylsalicylic acid (ASA, 100 mg/kg). V *P<0.001 a significant reduction in the number of writhing compared to the vehicle. Similarly, the percent of inhibition caused by TMNB was also significantly different from the vehicle, with #P<0.001.